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Silver Staining Protocol

Silver Staining Protocol

 

1x 40min - overnight 50% MeOH, 12% Acetic Acid

1x 30min 50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde

3x 20min 50% ETOH

1x 0.2min (variable) 200 mg/l Na2S2O3 5H2O

2x 2-3min ddH2O

1x 20min 2 g/l AgNO3, 750ul/l 37% Formaldehyde

1x 2-3min ddH2O

variable 60 g/l Na2CO3, 5 mg/l Na2S2O3 5H2O, 500ul/l 37% Formaldehyde

1x 2-3min ddH2O

1x 10min 50% MeOH, 12% Acetic Acid

1x 30min overnight 20% ETOH, 2% Glycerol