Silver Staining Protocol

 

1x 40min - overnight     50% MeOH, 12% Acetic Acid

1x 30min                                  50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde

3x 20min                                  50% ETOH

1x 0.2min (variable)      200 mg/l Na₂S₂O₃ ·5H₂O

2x 2-3min                                 ddH₂O

1x 20min                                  2 g/l AgNO₃, 750ul/l 37% Formaldehyde

1x 2-3min                                 ddH₂O

variable                                    60 g/l Na₂CO₃, 5 mg/l Na₂S₂O₃ ·5H₂O, 500ul/l 37% Formaldehyde

1x 2-3min                                 ddH₂O

1x 10min                                  50% MeOH, 12% Acetic Acid

1x 30min – overnight    20% ETOH, 2% Glycerol