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Use of Transmission Electron Microscopy
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  Author Information
 
  Primary Author
  William H. Heidcamp ( wheidcamp@gac.edu )
 
  Affiliation
  Gustavus Adolphus College , United States
 
  Co-Author(s)
   
 
 
  Protocol Information
 
  Protocol ID / PID
  PUB47
 
  Category
  Cell Biology
 
  Title
  Use of Transmission Electron Microscopy  
 
  Overview
  A protocol describing the use of Zeiss EM9-S transmission electron microscopy is presented. 
 
  Material
  Zeiss EM9-S transmission electron microscope  
 
  Procedure
  1. Familiarize yourself with the basic operations of Fixation, embedding and sectioning of materials for viewing in an electron microscope.

2. Insert and Remove a Specimen from the chamber.

3. Turn on the high voltage, check current levels and turn on the beam.

4. Find and center the beam.

5. Use magnification controls and condenser.

6. Use binocular viewing screen

7. Take an electron photomicrograph.
 
 
  Troubleshooting
   
 
  Reference
  Dr. William H. Heidcamp,
Biology Department,
Gustavus Adolphus College,
St. Peter, MN 56082 -- cellab@gac.edu
(http://www.gac.edu/~wheidcam/)  
 
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