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Viral Immunity and Pathogenesis Group On-line/ Platelet-related protocols / Spleenectomy
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Spleenectomy ]
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Universite Catholique de Louvain (UCL)
Institute for Cellular Pathology (ICP)
Experimental Medicine Unit (MEXP)
Viral Immunity&Pathogenesis Group


UCL-ICP-MEXP 7430

Av. Hippocrate, 74
1200 Bruxelles
Belgique

tel 32 2 764 7437
fax 32 2 764 7430
E-mail coutelier@mexp.ucl.ac.be



OUTLINE
PROTOCOL
SOLUTIONS
ADDITIONAL INFO
REFERENCES
OUTLINE
PROTOCOL

General anesthesia
1. inject i.p. 250µl/mouse of the ANESTHETIC mixture < wait for 2-10min for the anesthetic effect>
2. for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet)

Preparation of the surgical area
1. wash the surgical and adjacent area with C 2H5OH-70%
2. raze the fur with the razor
3. wash the surgical area withC2 H 5 OH-70%

Operation
  1. At the left hypochondrium make a 1.5-2cm "skin, muscles and peritoneum" incision with surgical scissors
  2. Gently trap and push the spleen free of adjacent tissue, separate its leg with blunt-ended scissors or forceps
  3. By a single knot tie off the artery with a 3-0 plane CATGUT by looping the suture through the mesentary (the artery enters the spleen at the superior polus). If necessary by a single knot tie off the efferent venule (exits the spleen at the inferior polus)
  4. Cut away the connective tissue and remove the spleen
  5. Apply on the open wound 1 pinch of Astrexene (Chlorhexidin HCl) (5mg/g)
  6. Apply on the peritoneum and muscles the U-like suture with plane CATGUT (3-0)
  7. Apply on the skin the U-like suture with ETHICON MERSILENE (3-0)

Wakening
1.  inject 250ul/mouse of the "WAKENING MIXTURE"

Post operation period
  1. after the operation took place let the animals to rest for at least 7 days in a cage with the filter
  2. observe animals every day


  SOLUTIONS

1. ANESTHETIC MIXTURE=mix 1ml of Domitor (Medetomidin HCl) (1mg/ml) , 1ml of Imalgene (Ketaminum HCl) (50mg/ml) and 8ml of PBS <all sterile>
2. "WAKENING MIXTURE"=mix 1ml of ANTISEDAN (Atipamezole HCl) (5mg/ml) and 9ml of PBS <all sterile!>

  ADDITIONAL INFO
  REFERENCES

created by Andrei Musaji  updated : 2003