Archived Protocol

<!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN"> <html> <head> <title>Viral Immunity and Pathogenesis Group On-line/ Platelet-related protocols / Spleenectomy</title> <meta http-equiv="content-type" content="text/html; charset=ISO-8859-1"> <meta name="author" content="Andrei Musaji"> <meta name="description" content="Platelets, Protocols, Spleenectomy, Virology, Immunology, Hematology, Autoimmunity"> </head> <body text="#000000" bgcolor="#ffffff" link="#3333ff" vlink="#009900" alink="#ff0000"> <div align="right"> <table align="center" width="100%" bgcolor="#ffffff"> <tbody> <tr> <td valign="top" height="55%" width="55%" bgcolor="#ffffff"><img src=http://www.icp.ucl.ac.be/mexp/WeDo.gif alt="The science we do" width="416" height="39"> </td> <td height="3" valign="top" bgcolor="#ffffff"> <div align="left"><a name="top"></a> </div> <div align="left"> </div> </td> </tr> <tr> <td valign="top"> </td> <td valign="top" bgcolor="#ffffcc"> <div align="center"> <div align="center">[<a href="http://www.ucl.ac.be">UCL</a>]>[<a href="http://www.icp.ucl.ac.be/">ICP</a>]>[<a href="index.html">MEXP</a>]>[VIPG Group <a href="vipg_home.html"> HOME PAGE</a>]> [VIPG Group <a href=http://www.icp.ucl.ac.be/mexp/vipg_protocols.html> PROTOCOLS</a>><a href="vipg_platelets.html">Platelet-Related</a>><a href="vipg_plateletsplect.html">Spleenectomy</a> ] </div> </div> <div align="left"> </div> </td> </tr> </tbody> </table> <div align="center"> </div> </div> <table width="100%"> <tbody> <tr> <td height="20%" width="20%"><img src=http://www.icp.ucl.ac.be/mexp/vipg.gif alt="Viral Immunity and Pathogenesis Group on-line" width="238" height="103" hspace="0" vspace="0" border="0"> </td> <td valign="top" bgcolor="#ffffcc"> <div align="left"> <big><big> </big></big><big><big><big><big><big><big><big><big>[</big></big></big></big></big></big></big></big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><a href=http://www.icp.ucl.ac.be/mexp/vipg_protocols.html><big><big><big><big>PROTOCOLS</big></big></big></big></a><big><big><big><big>><a href="vipg_platelets.html">PLATELET-RELATE</a></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><a href="vipg_platelets.html">D</a>></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big><big><big><big><big>SPLEENECTOMY</big></big></big></big><big><big><big><big><big><big><big><big><big><big><big><big>]</big></big></big></big></big></big></big></big></big></big></big></big> </div> </td> </tr> <tr> <td valign="top" height="20%" width="20%" bgcolor="#ffffcc"><a href="file:///Macintosh%20HD/Desktop%20Folder/VIPG-2003/vipg_home.html"><a href="vipg_home.html"><img src=http://www.icp.ucl.ac.be/mexp/home.gif alt="home" width="159" height="31" hspace="0" vspace="0" border="0"> </a> </a> <a href="file:///Macintosh%20HD/Desktop%20Folder/VIPG-2003/vipg_proj.html"><a href="vipg_proj.html"><img src=http://www.icp.ucl.ac.be/mexp/Projects.gif alt="projects" width="159" height="31" hspace="0" vspace="0" border="0"> </a> </a> <a href="file:///Macintosh%20HD/Desktop%20Folder/VIPG-2003/vipg_pub.html"><a href="vipg_pub.html"><img src=http://www.icp.ucl.ac.be/mexp/PUB.gif alt="publications" width="159" height="31" border="0"> </a> </a> <a href="file:///Macintosh%20HD/Desktop%20Folder/VIPG-2003/vipg_journ.html"><a href="vipg_journ.html"><img src=http://www.icp.ucl.ac.be/mexp/journals.gif alt="journals" width="159" height="31" hspace="0" vspace="0" border="0"> </a> </a> <a href="file:///Macintosh%20HD/Desktop%20Folder/VIPG-2003/vipg_staff.html"><a href="vipg_staff.html"><img src=http://www.icp.ucl.ac.be/mexp/Staff.gif alt="staff" width="159" height="31" hspace="0" vspace="0" border="0"> </a> </a> <a href="file:///Macintosh%20HD/Desktop%20Folder/VIPG-2003/vipg_map.html"><a href="vipg_map.html"><img src=http://www.icp.ucl.ac.be/mexp/sitemap.gif alt="sitemap" width="159" height="31" hspace="0" vspace="0" border="0"> </a> </a> <table cellpadding="0" cellspacing="0" border="0" width="100%"> <tbody> <tr> <td valign="top"><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big><big> Universite Catholique de Louvain (UCL) Institute for Cellular Pathology (ICP) Experimental Medicine Unit (MEXP) Viral Immunity&Pathogenesis Group</big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big></big> UCL-ICP-MEXP 7430 Av. Hippocrate, 74 1200 Bruxelles Belgique tel 32 2 764 7437 fax 32 2 764 7430 E-mail coutelier@mexp.ucl.ac.be </td> </tr> </tbody> </table> </td> <td valign="top"> <div align="justify"> <a href=http://www.icp.ucl.ac.be/mexp/vipg_plateletsplect.html#OUTLINE> OUTLINE</a> <a href=http://www.icp.ucl.ac.be/mexp/vipg_plateletsplect.html#PROTOCOL> PROTOCOL</a> <a href=http://www.icp.ucl.ac.be/mexp/vipg_plateletsplect.html#SOLUTIONS> SOLUTIONS </a> <a href=http://www.icp.ucl.ac.be/mexp/vipg_plateletsplect.html#ADDITIONAL_INFO> ADDITIONAL INFO</a> <a href=http://www.icp.ucl.ac.be/mexp/vipg_plateletsplect.html#REFERENCES_> REFERENCES</a> <hr width="100%"><a name="OUTLINE"></a> OUTLINE <a name="PROTOCOL"></a> PROTOCOL General anesthesia 1. inject i.p. 250µl/mouse of the <a href="#ANESTHETIC_MIXTURE"> ANESTHETIC mixture</a> < wait for 2-10min for the anesthetic effect> 2. for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation of the surgical area 1. wash the surgical and adjacent area with C 2H5OH-70% 2. raze the fur with the razor 3. wash the surgical area withC2 H 5 OH-70% Operation <ol> <li> At the left hypochondrium make a 1.5-2cm "skin, muscles and peritoneum" incision with surgical scissors</li> <li>Gently trap and push the spleen free of adjacent tissue, separate its leg with blunt-ended scissors or forceps</li> <li>By a single knot tie off the artery with a 3-0 plane CATGUT by looping the suture through the mesentary (the artery enters the spleen at the superior polus). If necessary by a single knot tie off the efferent venule (exits the spleen at the inferior polus)</li> <li>Cut away the connective tissue and remove the spleen</li> <li>Apply on the open wound 1 pinch of Astrexene (Chlorhexidin HCl) (5mg/g)</li> <li>Apply on the peritoneum and muscles the U-like suture with plane CATGUT (3-0)</li> <li>Apply on the skin the U-like suture with ETHICON MERSILENE (3-0) </li> </ol> Wakening 1.  inject 250ul/mouse of the <a href="#WAKENING_MIXTURE">"WAKENING MIXTURE" </a> Post operation period <ol> <li> after the operation took place let the animals to rest for at least 7 days in a cage with the filter</li> <li>observe animals every day </li> </ol>  <a name="SOLUTIONS"></a> SOLUTIONS 1. <a name="ANESTHETIC_MIXTURE"></a> ANESTHETIC MIXTURE=mix 1ml of Domitor (Medetomidin HCl) (1mg/ml) , 1ml of Imalgene (Ketaminum HCl) (50mg/ml) and 8ml of PBS <all sterile> 2. <a name="WAKENING_MIXTURE"></a> "WAKENING MIXTURE"=mix 1ml of ANTISEDAN (Atipamezole HCl) (5mg/ml) and 9ml of PBS <all sterile!>  <a name="ADDITIONAL_INFO"></a> ADDITIONAL INFO <div align="left"> <a name="REFERENCES_"></a> REFERENCES </div> </div> </td> </tr> </tbody> </table> <table cellpadding="2" cellspacing="2" border="0" width="25%" align="right"> <tbody> <tr> <td valign="top" bgcolor="#ffffcc"> <div align="center">created by <a href="mailto:Andrei.Musaji@mexp.ucl.ac.be"> Andrei Musaji</a>  updated : 2003 </div> </td> </tr> </tbody> </table> </body> </html>