50 mM Cacodylate, pH 7.4 (note: cacodylate contains arsenic, so handle carefully)
1% Uranyl acetate in water
Osmium tetroxide and glutaraldehyde can be purchased from the EM lab.
The plastics used to embed the tissue are more dangerous than the fixatives and many of the components of the plastic have been shown to cause cancer in rats or mice. During the embedding process the plastics are dissolved in solvent that can easily carry the plastic into your tissues and through any plastic gloves you may wear. In contrast to fixatives, whose actions are immediate and apparent, the consequences of exposure to the plastic resins are not apparent for years. Please be careful with the plastic resins prior to polymerization into hard blocks. Plastic-containing waste solutions should be kept in a container in the EM lab.
Another popular resin is Spurr resin, mixed as follows:
VCD 5 g DER 736 3 g NSA 13 g DMAE 0.2 g
Mix well and transfer to scintillation vials for storage.