This is a cached page for the URL (http://molbiol.ru/eng/protocol/01_02.html). To see the most recent version of this page, please click here.
Protocol Online is not affiliated with the authors of this page nor responsible for its content.
About Cache
   Salt solutions | molbiol.ru   
molbiol.ru - main page.
 > Protocols >  Solutions > Salt solutions.

Salt solutions (part one).

Rambler's Top100
AcO-          --- part one ---
(AcO)2Ca 1; 1.5M
AcOK 1; 2; 3; 4; 5; 6; 7; 8M
(AcO)2Mg 1M
AcONa 1; 2; 3; 4M
pH of Ac K/Na solutions
AcONH4 1; 2; 3; 4M
CO3-
K2CO3 1; 2; 3; 4; 5; 6M
KHCO3 1M
Na2CO3 1; 2M
NaHCO3 1M
(NH4)2CO3 1; 2; 3; 4; 5M
Br-
KBr 1; 2; 3; 4; 4.5M
NaBr 1; 2; 3; 4; 5.5M
Cl-
CaCl2 1; 2; 3; 4; 5M
CoCl2 1; 1.5M
CsCl 1; 2; 3; 4; 5; 6; 7M
KCl 1; 2; 3; 3.5M
LiCl 1; 2; 3; 4; 5; 6; 7; 8; 9; 10; 11; 12M
MgCl2 1; 2; 3; 4M
MnCl2 1; 2; 3M
NaCl 1; 2; 3; 4; 5M
NH4Cl 1; 2; 3; 4; 5M
NiCl2 1; 1.5M
RbCl 1; 2; 3; 4; 5; 6M
ZnCl2 1; 2; 3; 4; 5; 6; 7; 8; 9; 10M
ClO4-
NaClO4 1; 2; 3; 4M
 Supplement.
SxOy2-          --- part two ---
Cs2SO4 1M
CuSO4 1M
K2SO4 0.5M
KHSO4 1; 2M
MgSO4 1; 2; 2.5M
MnSO4 1; 2; 2.5M
Na2SO3 1; 1.5M
Na2S2O3 1; 2; 3M
Na2SO4 1M
NaHSO4 1; 2M
(NH4)2SO4 1; 2; 3; 4; 4.5M
NiSO4 1M
ZnSO4 1; 2; 2.5M
NO3-
AgNO3 1; 2; 3; 4; 5; 6M
PO42-
I-
KI 1; 2; 3; 4; 5; 6M
NaI 1; 2; 3; 4; 5; 6; 7M
Other:
NaN3 1; 2; 3; 4; 5M
KMnO4 0.5M
Na3C6H5O7 1M
Na3C6H5O7 1M
The densities of solutions were taken from "Reference book of chemist". (1964) Volume III, "Chemistry", Moscow-Leningrad, 2nd edition, Ed. B.P.Nikolskij. The original data are available on the page "Densities of salt solutions".








    About the recalculation of recipes for the arbitrary volumes:


    AcO-

    (AcO)2Ca

    Calcium Acetate, C4H6CaO4, Mw=158.17g/M
    Conc.Stock%(w/w)50ml100ml250ml
    (AcO)2Ca1M158.2g/M14.6067.91g15.82g39.55g
    H2O mQ85.39446.245ml92.49ml231.23ml
    1000ml/
    158.2g/M1M1.5M
    (AcO)2Ca [g]158.2
    237.3
    H2O [ml]924.9
    884.1
    %(w/w)14.60621.161
    H2O %(w/w)85.39478.839
    p (g/ml) 18oC1.08311.1214

    AcOK

    Potassium Acetate, KCH3CO2, Mw=98.15g/M; (store at NT).
    Conc.Stock%(w/w)50ml100ml150ml
    KC2H3O21M98.15g/M9.384.908g9.815g14.723g
    H2O mQ90.6247.42ml94.84ml142.3ml
    Conc.Stock%(w/w)50ml100ml150ml
    KC2H3O25M98.15g/M40.2924.54g49.08g73.61g
    H2O mQ59.7136.36ml72.72ml109.07ml
    1000ml/
    98.15g/M1M2M3M4M5M6M7M8M
    KC2H3O2 [g]98.15
    196.3
    294.45
    392.6
    490.75
    588.9
    687.05
    785.2
    H2O [ml]948.35
    895
    840.65
    784.6
    727.15
    668.2
    607.55
    545.7
    %(w/w)9.37917.98825.94033.35040.29546.84653.07058.998
    H2O %(w/w)90.62182.01274.06066.65059.70553.15446.93041.002
    p (g/ml) 18oC1.04651.09131.13511.17721.21791.25711.29461.3309

    (AcO)2Mg

    Magnesium Acetate, Mg(C2H3O2)2x 4H2O; Mw=214.46g/m; (store at NT).
    Conc.Stock50ml100ml300ml
    Mg(C2H3O2)2x4H2O1M214.46g/M10.72g21.45g64.34g
    H2O mQ    
    0.5M: 10.33%(w/w) Mg(C2H3O2)2x 4H2O, 89.67% H2O; p=1.038, 18oC;
    (none) 1M: 20.44%(w/w) Mg(C2H3O2)2x4H2O, 79.56% H2O; p=1.049.

    AcONa

    Sodium Acetate, Na(CH3COO) x 3H2O; Mw=136.08g/M; (store at NT).
    Conc.Stock50ml100ml300ml
    AcONax3H2O3M136.08g/M20.41g40.82g122.5g
    H2O mQ    
    Na(CH3COO); Mw=82.03g/M
    Conc.Stock50ml100ml300ml
    AcONa3M82.03g/M12.30g24.61g73.83g
    H2O mQ    
    pH5.2 - adjust by glacial acetic acid;
    pH7.0 - adjust by diluted acetic acid;
  • may be treated by DEPC and sterilized by autoclaving.
  • For 500ml of 3M NaAc
  • Desired pH
    500ml
    Glacial acetic acid
    5.905ml
    5.806ml
    5.5810ml
    5.2520ml
    5.1725ml
    5.0730ml
    1000ml/
    82.03g/M1M2M3M4M
    Na(CH3COO) [g]82.03
    164.06
    246.09
    328.12
    H2O [ml]956.67
    912.64
    867.41
    821.18
    %(w/w)7.89715.23722.10128.550
    H2O %(w/w)92.10384.76377.89971.450
    p (g/ml) 18oC1.03871.07671.11351.1493

    (none) Preparation of 0.1 M Sodium and Potassium Acetate buffers.

    Solution A: 5.75ml glacial acetic acid/liter (0.1M).
    Solution B: 0.1M monobasic Sodium/Potassium Acetate.    

    Desired pHSolution>ASolutionB
    SolutionASolutionB
    3.646.3ml3.7ml
    3.844.0ml6.0ml
    4.041.0ml9.0ml
    4.236.8ml13.2ml
    4.430.5ml19.5ml
    4.625.5ml24.5ml
    4.820.0ml30.0ml
    5.014.8ml35.2ml
    5.210.5ml39.5ml
    5.48.8ml41.2ml
    5.64.8ml45.2ml

    AcONH4

    Ammonium Acetate, CH3COONH4; Mw=77.08g/M; (store at NT).
    Conc.Stock%(w/w)10ml50ml150ml
    CH3COONH410M77.08g/M71.847.708g38.54g115.62g
    H2O mQ28.163.022ml15.11ml45.33ml
    10M: p=1.073.
    Conc.Stock%(w/w)50ml100ml200ml
    AcONH47.5M10M76.3037.5ml
    40.24g
    75ml
    80.48g
    150ml
    160.95g

    H2O mQ23.7012.5ml25ml50ml
    Conc.Stock%(w/w)50ml100ml200ml
    AcONH42.0M10M21.1510ml
    10.73g
    20ml
    21.46g
    40ml
    42.92g

    H2O mQ78.8540ml80ml160ml
    1000ml/
    77.08g/M1M2M3M4M
    AcONH4 [g]77.08
    154.16
    231.24
    308.32
    H2O [ml]938.02
    875.64
    811.96
    747.28
    %(w/w)7.59314.97022.16629.208
    H2O %(w/w)92.40785.03077.83470.792
    p (g/ml) 18oC1.01511.02981.04321.0556

    CO3-

    K2CO3

    Potassium Carbonate, K2CO3, Mw=138.2g/M;
    Conc.Stock%(w/w)50ml100ml250ml
    K2CO31M138.2g/M12.416.91g13.82g34.55g
    H2O mQ87.5948.77ml97.54ml243.85ml
    1000ml/
    138.2g/M1M2M3M4M5M6M
    K2CO3 [g]138.2
    276.4
    414.6
    552.8
    691
    829.2
    H2O [ml]975.4
    941.6
    900.4
    853.2
    802.2
    737.5
    %(w/w)12.41022.69331.52939.31746.27652.927
    H2O %(w/w)87.59077.30768.47160.68353.72447.073
    p (g/ml) 20oC1.11361.21801.31501.40601.49321.5667

    KHCO3

    Potassium Bicarbonate, KHCO3, Mw=100.1g/M;
    Conc.Stock%(w/w)50ml100ml250ml
    KHCO31M100.1g/M9.4145.005g10.01g25.03g
    H2O mQ90.58648.16ml96.32ml240.80ml
    1M; p=1.0633, 15oC

    Na2CO3

    Sodium Carbonate, Na2CO3, Mw=106.0g/M;
    Conc.Stock%(w/w)50ml100ml250ml
    Na2CO31M106.0g/M9.6465.30g10.60g26.50g
    H2O mQ90.35449.645ml99.29ml248.23ml
    1000ml/
    106.0g/M1M2M
    Na2CO3 [g]106
    212
    H2O [ml]992.9
    979.2
    %(w/w)9.64617.797
    H2O %(w/w)90.35482.203
    p (g/ml)1.09891.1912

    NaHCO3

    Sodium Bicarbonate, NaHCO3, Mw=84.01g/M; (store at NT).
    Conc.Stock%(w/w)50ml100ml250ml