Archived Protocol

<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN"> <html><head> <title>   Salt solutions | molbiol.ru   </title> <meta http-equiv=keywords name=keywords content="density, salt, acetate, carbonate, calcium, potassium, magnesium, sodium, ammonium, bicarbonate, bromide, chloride, cobaltous, cesium, lithium, manganese, nickel, rubidium, zinc, perchlorate, sulfate, cupric, bisulfate, sulfite, thiosulfate, hyposulfite, silver, phosphate, monobasic, tribasic, dibasic, azide, permanganate, iodide, DNA, RNA, protein, gene, vector, genome, bacteria, virus, cell, cloning, experiment, methods, tip, trick, expression, method, recipies, calculations"> <meta http-equiv=description name=description content="Composition of molecular biology solutions. Salt solutions. Practical Molecular Biology."> <meta name="Document-State" content="Static"> <meta name="Robots" content="ALL"><meta name="Robots" content="ALL"> <LINK rel="stylesheet" type="text/css" href="/style.css"> </head> <body background="/izo/fon.gif" topmargin="0" leftmargin="0" marginwidth="0" marginheight="0"> <a name="start"></a> <table width="800" border="0" cellspacing="0" cellpadding="0"> <tr><td colspan="2" width="610" valign="top">  <a href=http://molbiol.ru/eng/index.html><img src=http://molbiol.ru/izo/logo.gif width="98" height="41" alt="molbiol.ru - main page." border="0" align=left></a><img src=http://molbiol.ru/izo/1.gif width="13" height="13" border="0"><br><font color="Gray" size="-1"> > <a href=http://molbiol.ru/eng/protocol/index.html>Protocols</a> >  <a href=http://molbiol.ru/eng/protocol/index.html>Solutions</a> > <b>Salt solutions.</font></b> <hr align="left" size="2" color="Gray" noshade> <p class="h1"> Salt solutions (part one). </p></td><td rowspan="3" width="190" valign="top"> <div class="enav"><br> <FORM METHOD="GET" ACTION="/cgi/gliws.pl/frm/search"> <INPUT TYPE="text" NAME="query" SIZE=15><br> <INPUT TYPE="submit" NAME="Search" VALUE="Find"><br> <hr align="left" width="125" size="2" color="Gray" noshade> <a href=http://molbiol.ru/solution/03_01.html><img src=http://molbiol.ru/izo/rus.gif width="32" height="21" alt="Russian version" border="0"></a>  <a href=http://molbiol.ru/ger/protocol/01_02.html><img src=http://molbiol.ru/izo/ger.gif width="32" height="21" alt="Deutsche Version" border="0"></a>  <a href=http://molbiol.ru/ukr/protocol/01_02.html><img src=http://molbiol.ru/izo/ukr.gif width="32" height="21" alt="Óêðà¿íñüêèé âàð³àíò" border="0"></a>  <br><hr align="left" width="125" size="2" color="Gray" noshade> <a href=http://molbiol.ru/eng/index.html>Main page.</a><br> <a href=http://molbiol.ru/eng/project.html>Project.</a><br> <a href=http://molbiol.ru/eng/protocol/><b>Protocols.</b></a><br>    <a href=http://molbiol.ru/eng/protocol/index.html><font size="-1"><b>§ Solutions.</b></font></a><br> <a href=http://molbiol.ru/eng/scripts/>Calculations.</a><br> <a href=http://molbiol.ru/cgi-bin/biomail/users.pl>BioMail.</a><br> <a href=http://molbiol.ru/eng/journals/>Literature.</a><br> <a href=http://molbiol.ru/eng/workdb/>Labor exchange.</a><br> </FORM>  <table width="100" border="1" cellpadding="3" bordercolorlight="#FFFFFF"><tr align="center" valign="middle" bgcolor="#FFFFFF"><td><a href=http://molbiol.ru/review/index.html#_2a>Ñòðóêòóðíàÿ îðãàíèçàöèÿ áåëêà<br>Å.Ì.Ïîïîâ</td></tr></table> </div> <img src=http://counter.rambler.ru/top100.cnt?164472 alt="Rambler's Top100" width=1 height=1 border=0><img height=1 width=1 src=http://top.list.ru/counter?id=339529><img src=http://cnt.one.ru/cgi-bin/cnt.cgi?id=145057 width=1 height=1 alt="" border=0> <script language="javascript"></script><noscript> <img src=http://u586.76.spylog.com/cnt?cid=58676&p=1 alt='SpyLOG' border='0' width=1 height=1 > </noscript>  </td></tr> <tr><td width="310" bgcolor="eeeeee" valign="top"> <div class="inav"> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a1'>AcO<SUP><font size="+1">-</font></SUP></A>          <font size="+1"><b>--- part one ---</b></font><br> <div class="ls"><A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a2'>(AcO)<sub>2</sub>Ca</A> 1; 1.5M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a3'>AcOK</A> 1; 2; 3; 4; 5; 6; 7; 8M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a4'>(AcO)<sub>2</sub>Mg</A> 1M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a5'>AcONa</A> 1; 2; 3; 4M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a5a'>pH of Ac K/Na solutions</A><br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a6'>AcONH<sub>4</sub></A> 1; 2; 3; 4M<br></div> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a7'>CO<SUB>3</SUB><SUP><font size="+1">-</font></SUP></A><br> <div class="ls"><A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a8'>K<sub>2</sub>CO<sub>3</sub></A> 1; 2; 3; 4; 5; 6M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a9'>KHCO<sub>3</sub></A> 1M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a10'>Na<sub>2</sub>CO<sub>3</sub></A> 1; 2M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a11'>NaHCO<sub>3</sub></A> 1M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a12'>(NH<sub>4</sub>)<sub>2</sub>CO<sub>3</sub></A> 1; 2; 3; 4; 5M<br></div> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a13'>Br<SUP><font size="+1">-</font></SUP></A><br> <div class="ls"><A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a14'>KBr</A> 1; 2; 3; 4; 4.5M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a15'>NaBr</A> 1; 2; 3; 4; 5.5M<br></div> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a16'>Cl<SUP><font size="+1">-</font></SUP></A><br> <div class="ls"><A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a17'>CaCl<sub>2</sub></A> 1; 2; 3; 4; 5M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a18'>CoCl<sub>2</sub></A> 1; 1.5M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a19'>CsCl</A> 1; 2; 3; 4; 5; 6; 7M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a20'>KCl</A> 1; 2; 3; 3.5M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a21'>LiCl</A> 1; 2; 3; 4; 5; 6; 7; 8; 9; 10; 11; 12M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a22'>MgCl<sub>2</sub></A> 1; 2; 3; 4M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a23'>MnCl<sub>2</sub></A> 1; 2; 3M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a24'>NaCl</A> 1; 2; 3; 4; 5M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a25'>NH<sub>4</sub>Cl</A> 1; 2; 3; 4; 5M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a26'>NiCl<sub>2</sub></A> 1; 1.5M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a27'>RbCl</A> 1; 2; 3; 4; 5; 6M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a28'>ZnCl<sub>2</sub></A> 1; 2; 3; 4; 5; 6; 7; 8; 9; 10M<br></div> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a29'>ClO<sub>4</sub><SUP><font size="+1">-</font></SUP></A><br> <div class="ls"><A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02.html#a30'>NaClO<sub>4</sub></A> 1; 2; 3; 4M<br></div> <A href=http://molbiol.ru/eng/protocol/01_02.html#add><img src=http://molbiol.ru/izo/k.gif width="11" height="9" alt="" border="0"> Supplement.</A><br><font color="Gray"> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a31'>S<sub>x</sub>O<SUB>y</SUB><SUP>2<font size="+1">-</font></SUP></A>          <font size="+1"><b>--- part two ---</b></font><br> <div class="ls"><A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a43'>Cs<sub>2</sub>SO<sub>4</sub></A> 1M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a32'>CuSO<sub>4</sub></A> 1M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a33'>K<sub>2</sub>SO<sub>4</sub></A> 0.5M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a34'>KHSO<sub>4</sub></A> 1; 2M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a35'>MgSO<sub>4</sub></A> 1; 2; 2.5M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a36'>MnSO<sub>4</sub></A> 1; 2; 2.5M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a37'>Na<sub>2</sub>SO<sub>3</sub></A> 1; 1.5M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a38'>Na<sub>2</sub>S<sub>2</sub>O<sub>3</sub></A> 1; 2; 3M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a39'>Na<sub>2</sub>SO<sub>4</sub></A> 1M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a40'>NaHSO<sub>4</sub></A> 1; 2M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a41'>(NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub></A> 1; 2; 3; 4; 4.5M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a42'>NiSO<sub>4</sub></A> 1M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a44'>ZnSO<sub>4</sub></A> 1; 2; 2.5M<br></div> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a45'>NO<sub>3</sub><sup><font size="+1">-</font></sup></A><br> <div class="ls"><A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a46'>AgNO<sub>3</sub></A> 1; 2; 3; 4; 5; 6M<br></div> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a47'>PO<sub>4</sub><sup>2<font size="+1">-</font></sup></A><br> <div class="ls"><A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a48'>KH<sub>2</sub>PO<sub>4</sub></A> 1M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a48a'>pH of KP solutions</A> 1M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a49'>Na<sub>3</sub>PO<sub>4</sub></A> 0.5M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a50'>Na<sub>2</sub>HPO<sub>4</sub></A> 0.5M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a51'>NaH<sub>2</sub>PO<sub>4</sub></A> 1M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a51a'>pH of NaP solutions</A> 1M<br></div> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a52'>I<sup><font size="+1">-</font></sup></A><br> <div class="ls"><A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a53'>KI</A> 1; 2; 3; 4; 5; 6M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a54'>NaI</A> 1; 2; 3; 4; 5; 6; 7M<br></div> Other:<br> <div class="ls"><A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a55'>NaN<sub>3</sub></A> 1; 2; 3; 4; 5M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a56'>KMnO<sub>4</sub></A> 0.5M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a57'>Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub></A> 1M<br> <A href=http://molbiol.ru/eng/protocol/'/eng/protocol/01_02b.html#a58'>Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub></A> 1M<br></div> </font> </div> </TD><td valign="top"> <div class="epig"> The densities of solutions were taken from "Reference book of chemist". (1964) Volume III, "Chemistry", Moscow-Leningrad, 2nd edition, Ed. B.P.Nikolskij. The original data are available on the page "<a href=http://molbiol.ru/eng/protocol/01_22.html>Densities of salt solutions</a>". </div> <br><br><br><br><br><br><br><br> <ul><form name="hlp" id="hlp"><center>About the recalculation of recipes for the arbitrary volumes:<br><br><INPUT TYPE="button" VALUE="Help" onClick="helpw()"></center></form></ul> </td></tr> <tr><td width=610 colspan="2" valign="top"> </td></tr></table> <table width="610" border="0" cellspacing="0" cellpadding="0"><tr><td> <script language="JavaScript" src=http://molbiol.ru/scripts/solution.js type="text/javascript"></script> <script language="JavaScript" src=http://molbiol.ru/eng/scripts/sol_a.js type="text/javascript"></script> <div class="prot"><br><ul type="disc"> <p class=h2><A name="a1"></A>AcO<SUP><font size="+1">-</font></SUP></P> <form><p class=h3><A name="a2">(AcO)<sub>2</sub>Ca</A></P> Calcium Acetate, C<sub>4</sub>H<sub>6</sub>CaO<sub>4</sub>, Mw=158.17g/M <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>%(w/w)</TD><TD>50ml</TD><TD>100ml</TD><TD>250ml</TD><TD align="center"><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>(AcO)<sub>2</sub>Ca</TD><TD>1M</TD><TD>158.2g/M</TD><TD>14.606</TD><TD>7.91g</TD><TD>15.82g</TD><TD>39.55g</TD><TD align="center"><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>H<sub>2</sub>O</TD><TD> </TD><TD>mQ</TD><TD>85.394</TD><TD>46.245ml</TD><TD>92.49ml</TD><TD>231.23ml</TD><TD align="center"><input type="text" size=7 onChange='clc(this,2)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,15.82,2,g,92.49,2,ml"></form> <form><TABLE width="350" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD colspan="2" align="center">1000ml/<input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR align=center bgcolor="eeeeee"><TD>158.2g/M</TD><TD>1M</TD><TD>1.5M</TD></TR> <TR align=center bgcolor="ffffff"><TD>(AcO)<sub>2</sub>Ca [g]</TD><TD>158.2<br><input type="text" size=7 onChange='clc(this,1)'></TD><TD>237.3<br><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>H<sub>2</sub>O [ml]</TD><TD>924.9<br><input type="text" size=7 onChange='clc(this,3)'></TD><TD>884.1<br><input type="text" size=7 onChange='clc(this,4)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>%(w/w)</TD><TD>14.606</TD><TD>21.161</TD></TR> <TR align=center bgcolor="ffffff"><TD>H<sub>2</sub>O %(w/w)</TD><TD>85.394</TD><TD>78.839</TD></TR> <TR align=center bgcolor="ffffff"><TD>p (g/ml) 18<SUP>o</SUP>C</TD><TD>1.0831</TD><TD>1.1214</TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,15.83,2,,23.73,2,,92.49,2,,88.41,2,"></form> <form><p class=h3><A name="a3"></A>AcOK</P> Potassium Acetate, KCH<SUB>3</SUB>CO<SUB>2</SUB>, Mw=98.15g/M; (store at NT).<br> <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>%(w/w)</TD><TD>50ml</TD><TD>100ml</TD><TD>150ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>KC<SUB>2</SUB>H<SUB>3</SUB>O<SUB>2</SUB></TD><TD>1M</TD><TD>98.15g/M</TD><TD>9.38</TD><TD>4.908g</TD><TD>9.815g</TD><TD>14.723g</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD>90.62</TD><TD>47.42ml</TD><TD>94.84ml</TD><TD>142.3ml</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,9.815,2,g,94.835,2,ml"></form> <form> <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>%(w/w)</TD><TD>50ml</TD><TD>100ml</TD><TD>150ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>KC<SUB>2</SUB>H<SUB>3</SUB>O<SUB>2</SUB></TD><TD>5M</TD><TD>98.15g/M</TD><TD>40.29</TD><TD>24.54g</TD><TD>49.08g</TD><TD>73.61g</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD>59.71</TD><TD>36.36ml</TD><TD>72.72ml</TD><TD>109.07ml</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,49.075,2,g,72.715,2,ml"></form> <form><TABLE width="580" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD colspan="8" align="center">1000ml/<input type="text" size=6 onChange='clc(this,0)'></TD></TR> <TR align=center bgcolor="eeeeee"><TD>98.15g/M</TD><TD>1M</TD><TD>2M</TD><TD>3M</TD><TD>4M</TD><TD>5M</TD><TD>6M</TD><TD>7M</TD><TD>8M</TD></TR> <TR align=center bgcolor="ffffff"><TD>KC<sub>2</sub>H<sub>3</sub>O<sub>2</sub> [g]</TD><TD>98.15<br><input type="text" size=6 onChange='clc(this,1)'></TD><TD>196.3<br><input type="text" size=6 onChange='clc(this,2)'></TD><TD>294.45<br><input type="text" size=6 onChange='clc(this,3)'></TD><TD>392.6<br><input type="text" size=6 onChange='clc(this,4)'></TD><TD>490.75<br><input type="text" size=6 onChange='clc(this,5)'></TD><TD>588.9<br><input type="text" size=6 onChange='clc(this,6)'></TD><TD>687.05<br><input type="text" size=6 onChange='clc(this,7)'></TD><TD>785.2<br><input type="text" size=6 onChange='clc(this,8)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>H<sub>2</sub>O [ml]</TD><TD>948.35<br><input type="text" size=6 onChange='clc(this,9)'></TD><TD>895<br><input type="text" size=6 onChange='clc(this,10)'></TD><TD>840.65<br><input type="text" size=6 onChange='clc(this,11)'></TD><TD>784.6<br><input type="text" size=6 onChange='clc(this,12)'></TD><TD>727.15<br><input type="text" size=6 onChange='clc(this,13)'></TD><TD>668.2<br><input type="text" size=6 onChange='clc(this,14)'></TD><TD>607.55<br><input type="text" size=6 onChange='clc(this,15)'></TD><TD>545.7<br><input type="text" size=6 onChange='clc(this,16)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>%(w/w)</TD><TD>9.379</TD><TD>17.988</TD><TD>25.940</TD><TD>33.350</TD><TD>40.295</TD><TD>46.846</TD><TD>53.070</TD><TD>58.998</TD></TR> <TR align=center bgcolor="ffffff"><TD>H<sub>2</sub>O %(w/w)</TD><TD>90.621</TD><TD>82.012</TD><TD>74.060</TD><TD>66.650</TD><TD>59.705</TD><TD>53.154</TD><TD>46.930</TD><TD>41.002</TD></TR> <TR align=center bgcolor="ffffff"><TD>p (g/ml) 18<SUP>o</SUP>C</TD><TD>1.0465</TD><TD>1.0913</TD><TD>1.1351</TD><TD>1.1772</TD><TD>1.2179</TD><TD>1.2571</TD><TD>1.2946</TD><TD>1.3309</TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value=100,2,ml,9.815,2,,19.63,2,,29.445,2,,39.26,2,,49.075,2,,58.89,2,,68.705,2,,78.52,2,,94.835,2,,89.5,2,,84.065,2,,78.46,2,,72.715,2,,66.82,2,,60.755,2,,54.57,2,></form> <form><p class=h3><A name="a4"></A>(AcO)<SUB>2</SUB>Mg</P> Magnesium Acetate, Mg(C<SUB>2</SUB>H<SUB>3</SUB>O<SUB>2</SUB>)<SUB>2</SUB>x 4H<SUB>2</SUB>O; Mw=214.46g/m; (store at NT). <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>50ml</TD><TD>100ml</TD><TD>300ml</TD><td><input type="text" size=7 onChange='clc(this,0)'></td></TR> <TR align=center bgcolor="ffffff"><TD>Mg(C<SUB>2</SUB>H<SUB>3</SUB>O<SUB>2</SUB>)<SUB>2</SUB>x4H<SUB>2</SUB>O</TD><TD>1M</TD><TD>214.46g/M</TD><TD>10.72g</TD><TD>21.45g</TD><TD>64.34g</TD><td><input type="text" size=7 onChange='clc(this,1)'></td></TR> <TR align=center bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD> </TD><TD> </TD><TD> </TD><td> </td></TR> </TABLE></TD></TR> </TABLE> 0.5M: 10.33%(w/w) Mg(C<SUB>2</SUB>H<SUB>3</SUB>O<SUB>2</SUB>)<SUB>2</SUB>x 4H<SUB>2</SUB>O, 89.67% H<sub>2</sub>O; p=1.038, 18<SUP>o</SUP>C;<br> <img src=http://molbiol.ru/eng/izo/trefa.gif alt="(none)" width="11" height="9" border="0"> 1M: 20.44%(w/w) Mg(C<SUB>2</SUB>H<SUB>3</SUB>O<SUB>2</SUB>)<SUB>2</SUB>x4H<SUB>2</SUB>O, 79.56% H<SUB>2</SUB>O; p=1.049.<input type="hidden" name="prm" value="100,2,ml,21.446,2,g"></form> <form><p class=h3><A name="a5"></A>AcONa</P> Sodium Acetate, Na(CH<SUB>3</SUB>COO) x 3H<SUB>2</SUB>O; Mw=136.08g/M; (store at NT). <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>50ml</TD><TD>100ml</TD><TD>300ml</TD><td><input type="text" size=7 onChange='clc(this,0)'></td></TR> <TR align=center bgcolor="ffffff"><TD>AcONax3H<SUB>2</SUB>O</TD><TD>3M</TD><TD>136.08g/M</TD><TD>20.41g</TD><TD>40.82g</TD><TD>122.5g</TD><td><input type="text" size=7 onChange='clc(this,1)'></td></TR> <TR align=center bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD> </TD><TD> </TD><TD> </TD><td> </td></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,40.824,2,g"></form> <form>Na(CH<SUB>3</SUB>COO); Mw=82.03g/M <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>50ml</TD><TD>100ml</TD><TD>300ml</TD><td><input type="text" size=7 onChange='clc(this,0)'></td></TR> <TR align=center bgcolor="ffffff"><TD>AcONa</TD><TD>3M</TD><TD>82.03g/M</TD><TD>12.30g</TD><TD>24.61g</TD><TD>73.83g</TD><td><input type="text" size=7 onChange='clc(this,1)'></td></TR> <TR align=center bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD> </TD><TD> </TD><TD> </TD><td> </td></TR> </TABLE></TD></TR> </TABLE> pH5.2 - adjust by glacial acetic acid;<br> pH7.0 - adjust by diluted acetic acid;<br> <li>may be treated by DEPC and sterilized by autoclaving.</li> <input type="hidden" name="prm" value="100,2,ml,24.609,2,g"></form> <form><li><b>For 500ml of 3M NaAc</b></li> <TABLE width="300" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align=center bgcolor="eeeeee"><TD rowspan=2>Desired pH<br>500ml</TD><TD rowspan=2>Glacial acetic acid</TD><td><input type="Reset" value='Clear'></td></TR> <TR align=center bgcolor="eeeeee"><td><input type="text" size=7 onChange='clc(this,0)'></td></TR> <TR align=center bgcolor="ffffff"><TD>5.90</TD><TD>5ml</TD><td><input type="text" size=7 onChange='clc(this,1)'></td></TR> <TR align=center bgcolor="ffffff"><TD>5.80</TD><TD>6ml</TD><td><input type="text" size=7 onChange='clc(this,2)'></td></TR> <TR align=center bgcolor="ffffff"><TD>5.58</TD><TD>10ml</TD><td><input type="text" size=7 onChange='clc(this,3)'></td></TR> <TR align=center bgcolor="ffffff"><TD>5.25</TD><TD>20ml</TD><td><input type="text" size=7 onChange='clc(this,4)'></td></TR> <TR align=center bgcolor="ffffff"><TD>5.17</TD><TD>25ml</TD><td><input type="text" size=7 onChange='clc(this,5)'></td></TR> <TR align=center bgcolor="ffffff"><TD>5.07</TD><TD>30ml</TD><td><input type="text" size=7 onChange='clc(this,6)'></td></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,1,2,ml,1.2,2,ml,2,2,ml,4,2,ml,5,2,ml,6,2,ml"></form> <form><TABLE width="450" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD colspan="4" align="center">1000ml/<input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR align=center bgcolor="eeeeee"><TD>82.03g/M</TD><TD>1M</TD><TD>2M</TD><TD>3M</TD><TD>4M</TD></TR> <TR align=center bgcolor="ffffff"><TD>Na(CH<sub>3</sub>COO) [g]</TD><TD>82.03<br><input type="text" size=6 onChange='clc(this,1)'></TD><TD>164.06<br><input type="text" size=6 onChange='clc(this,2)'></TD><TD>246.09<br><input type="text" size=6 onChange='clc(this,3)'></TD><TD>328.12<br><input type="text" size=6 onChange='clc(this,4)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>H<sub>2</sub>O [ml]</TD><TD>956.67<br><input type="text" size=6 onChange='clc(this,5)'></TD><TD>912.64<br><input type="text" size=6 onChange='clc(this,6)'></TD><TD>867.41<br><input type="text" size=6 onChange='clc(this,7)'></TD><TD>821.18<br><input type="text" size=6 onChange='clc(this,8)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>%(w/w)</TD><TD>7.897</TD><TD>15.237</TD><TD>22.101</TD><TD>28.550</TD></TR> <TR align=center bgcolor="ffffff"><TD>H<sub>2</sub>O %(w/w)</TD><TD>92.103</TD><TD>84.763</TD><TD>77.899</TD><TD>71.450</TD></TR> <TR align=center bgcolor="ffffff"><TD>p (g/ml) 18<SUP>o</SUP>C</TD><TD>1.0387</TD><TD>1.0767</TD><TD>1.1135</TD><TD>1.1493</TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,8.203,2,,16.406,2,,24.609,2,,32.812,2,,95.667,2,,91.264,2,,86.741,2,,82.118,2,"></form> <form><A name="a5a"></A><p class=h3><img src=http://molbiol.ru/izo/trefa.gif alt="(none)" width="11" height="9" border="0"> Preparation of 0.1 M Sodium and Potassium Acetate buffers.</P> Solution A: 5.75ml glacial acetic acid/liter (0.1M).<br> Solution B: 0.1M monobasic Sodium/Potassium Acetate.    <input type="Reset" value='Clear'><br><br> <TABLE width="450" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align=center bgcolor="eeeeee"> <TD rowspan="2">Desired pH</TD><TD rowspan="2">Solution>A</TD><TD rowspan="2">SolutionB</TD> <td colspan="2"><input type="text" size=7 onChange='clc(this,0)'></td></TR> <TR align=center bgcolor="eeeeee"><TD>SolutionA</TD><TD>SolutionB</TD></TR> <TR align=center bgcolor="ffffff"><TD>3.6</TD><TD>46.3ml</TD><TD>3.7ml</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>3.8</TD><TD>44.0ml</TD><TD>6.0ml</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD><TD><input type="text" size=7 onChange='clc(this,4)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>4.0</TD><TD>41.0ml</TD><TD>9.0ml</TD><TD><input type="text" size=7 onChange='clc(this,5)'></TD><TD><input type="text" size=7 onChange='clc(this,6)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>4.2</TD><TD>36.8ml</TD><TD>13.2ml</TD><TD><input type="text" size=7 onChange='clc(this,7)'></TD><TD><input type="text" size=7 onChange='clc(this,8)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>4.4</TD><TD>30.5ml</TD><TD>19.5ml</TD><TD><input type="text" size=7 onChange='clc(this,9)'></TD><TD><input type="text" size=7 onChange='clc(this,10)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>4.6</TD><TD>25.5ml</TD><TD>24.5ml</TD><TD><input type="text" size=7 onChange='clc(this,11)'></TD><TD><input type="text" size=7 onChange='clc(this,12)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>4.8</TD><TD>20.0ml</TD><TD>30.0ml</TD><TD><input type="text" size=7 onChange='clc(this,13)'></TD><TD><input type="text" size=7 onChange='clc(this,14)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>5.0</TD><TD>14.8ml</TD><TD>35.2ml</TD><TD><input type="text" size=7 onChange='clc(this,15)'></TD><TD><input type="text" size=7 onChange='clc(this,16)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>5.2</TD><TD>10.5ml</TD><TD>39.5ml</TD><TD><input type="text" size=7 onChange='clc(this,17)'></TD><TD><input type="text" size=7 onChange='clc(this,18)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>5.4</TD><TD>8.8ml</TD><TD>41.2ml</TD><TD><input type="text" size=7 onChange='clc(this,19)'></TD><TD><input type="text" size=7 onChange='clc(this,20)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>5.6</TD><TD>4.8ml</TD><TD>45.2ml</TD><TD><input type="text" size=7 onChange='clc(this,21)'></TD><TD><input type="text" size=7 onChange='clc(this,22)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,92.6,2,ml,7.4,2,ml,88,2,ml,12,2,ml,82,2,ml,18,2,ml,73.6,2,ml,26.4,2,ml,61,2,ml,39,2,ml,51,2,ml,49,2,ml,40,2,ml,60,2,ml,29.6,2,ml,70.4,2,ml,21,2,ml,79,2,ml,17.6,2,ml,82.4,2,ml,9.6,2,ml,90.4,2,ml"></form> <form><p class=h3><A name="a6"></A>AcONH<SUB>4</SUB></P> Ammonium Acetate, CH<sub>3</sub>COONH<sub>4</sub>; Mw=77.08g/M; (store at NT). <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>%(w/w)</TD><TD>10ml</TD><TD>50ml</TD><TD>150ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>CH<sub>3</sub>COONH<SUB>4</SUB></TD><TD>10M</TD><TD>77.08g/M</TD><TD>71.84</TD><TD>7.708g</TD><TD>38.54g</TD><TD>115.62g</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD>28.16</TD><TD>3.022ml</TD><TD>15.11ml</TD><TD>45.33ml</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> </TABLE></TD></TR> </TABLE> 10M: p=1.073.<input type="hidden" name="prm" value="100,2,ml,77.08,2,g,30.22,2,ml"></form> <form><TABLE width="480" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><td>%(w/w)</td><TD>50ml</TD><TD>100ml</TD><TD>200ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>AcONH<SUB>4</SUB></TD><TD>7.5M</TD><TD>10M</TD><td>76.30</td><TD><U>37.5ml</U><br>40.24g</TD><TD><U>75ml</U><br>80.48g</TD><TD><U>150ml</U><br>160.95g</TD><TD><input type="text" size=7 onChange='clc(this,1)'><br><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><td>23.70</td><TD>12.5ml</TD><TD>25ml</TD><TD>50ml</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,75,2,ml,80.475,2,g,25,2,ml"></form> <form><TABLE width="480" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><td>%(w/w)</td><TD>50ml</TD><TD>100ml</TD><TD>200ml</TD><td><input type="text" size=7 onChange='clc(this,0)'></td></TR> <TR align=center bgcolor="ffffff"><TD>AcONH<SUB>4</SUB></TD><TD>2.0M</TD><TD>10M</TD><td>21.15</td><TD><U>10ml</U><br>10.73g</TD><TD><U>20ml</U><br>21.46g</TD><TD><U>40ml</U><br>42.92g</TD><td><input type="text" size=7 onChange='clc(this,1)'><br><input type="text" size=7 onChange='clc(this,2)'></td></TR> <TR align=center bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><td>78.85</td><TD>40ml</TD><TD>80ml</TD><TD>160ml</TD><td><input type="text" size=7 onChange='clc(this,3)'></td></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,20.0,2,ml,21.46,2,g,80,2,ml"></form> <form><TABLE width="480" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD colspan="4" align="center">1000ml/<input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR align=center bgcolor="eeeeee"><TD>77.08g/M</TD><TD>1M</TD><TD>2M</TD><TD>3M</TD><TD>4M</TD></TR> <TR align=center bgcolor="ffffff"><TD>AcONH<sub>4</sub> [g]</TD><TD>77.08<br><input type="text" size=6 onChange='clc(this,1)'></TD><TD>154.16<br><input type="text" size=6 onChange='clc(this,2)'></TD><TD>231.24<br><input type="text" size=6 onChange='clc(this,3)'></TD><TD>308.32<br><input type="text" size=6 onChange='clc(this,4)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>H<sub>2</sub>O [ml]</TD><TD>938.02<br><input type="text" size=6 onChange='clc(this,5)'></TD><TD>875.64<br><input type="text" size=6 onChange='clc(this,6)'></TD><TD>811.96<br><input type="text" size=6 onChange='clc(this,7)'></TD><TD>747.28<br><input type="text" size=6 onChange='clc(this,8)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>%(w/w)</TD><TD>7.593</TD><TD>14.970</TD><TD>22.166</TD><TD>29.208</TD></TR> <TR align=center bgcolor="ffffff"><TD>H<sub>2</sub>O %(w/w)</TD><TD>92.407</TD><TD>85.030</TD><TD>77.834</TD><TD>70.792</TD></TR> <TR align=center bgcolor="ffffff"><TD>p (g/ml) 18<SUP>o</SUP>C</TD><TD>1.0151</TD><TD>1.0298</TD><TD>1.0432</TD><TD>1.0556</TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,7.708,2,,15.416,2,,23.124,2,,30.832,2,,93.802,2,,87.564,2,,81.196,2,,74.728,2,"></form> </ul></div> </td></tr></table> <table width="610" border="0" cellspacing="0" cellpadding="0"><tr><td> <div class="prot"><br><ul type="disc"> <p class=h2><A name="a7"></A>CO<SUB>3</SUB><SUP><font size="+1">-</font></SUP></P> <form><p class=h3><A name="a8"></A>K<sub>2</sub>CO<sub>3</sub></P> Potassium Carbonate, K<sub>2</sub>CO<sub>3</sub>, Mw=138.2g/M; <br> <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>%(w/w)</TD><TD>50ml</TD><TD>100ml</TD><TD>250ml</TD><TD align="center"><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>K<sub>2</sub>CO<sub>3</sub></TD><TD>1M</TD><TD>138.2g/M</TD><TD>12.41</TD><TD>6.91g</TD><TD>13.82g</TD><TD>34.55g</TD><TD align="center"><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>H<sub>2</sub>O</TD><TD> </TD><TD>mQ</TD><TD>87.59</TD><TD>48.77ml</TD><TD>97.54ml</TD><TD>243.85ml</TD><TD align="center"><input type="text" size=7 onChange='clc(this,2)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,13.820,2,g,97.54,2,ml"></form> <form><TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD colspan="6" align="center">1000ml/<input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR align=center bgcolor="eeeeee"><TD>138.2g/M</TD><TD>1M</TD><TD>2M</TD><TD>3M</TD><TD>4M</TD><TD>5M</TD><TD>6M</TD></TR> <TR align=center bgcolor="ffffff"><TD>K<sub>2</sub>CO<sub>3</sub> [g]</TD><TD>138.2<br><input type="text" size=6 onChange='clc(this,1)'></TD><TD>276.4<br><input type="text" size=6 onChange='clc(this,2)'></TD><TD>414.6<br><input type="text" size=6 onChange='clc(this,3)'></TD><TD>552.8<br><input type="text" size=6 onChange='clc(this,4)'></TD><TD>691<br><input type="text" size=6 onChange='clc(this,5)'></TD><TD>829.2<br><input type="text" size=6 onChange='clc(this,6)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>H<sub>2</sub>O [ml]</TD><TD>975.4<br><input type="text" size=6 onChange='clc(this,7)'></TD><TD>941.6<br><input type="text" size=6 onChange='clc(this,8)'></TD><TD>900.4<br><input type="text" size=6 onChange='clc(this,9)'></TD><TD>853.2<br><input type="text" size=6 onChange='clc(this,10)'></TD><TD>802.2<br><input type="text" size=6 onChange='clc(this,11)'></TD><TD>737.5<br><input type="text" size=6 onChange='clc(this,12)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>%(w/w)</TD><TD>12.410</TD><TD>22.693</TD><TD>31.529</TD><TD>39.317</TD><TD>46.276</TD><TD>52.927</TD></TR> <TR align=center bgcolor="ffffff"><TD>H<sub>2</sub>O %(w/w)</TD><TD>87.590</TD><TD>77.307</TD><TD>68.471</TD><TD>60.683</TD><TD>53.724</TD><TD>47.073</TD></TR> <TR align=center bgcolor="ffffff"><TD>p (g/ml) 20<SUP>o</SUP>C</TD><TD>1.1136</TD><TD>1.2180</TD><TD>1.3150</TD><TD>1.4060</TD><TD>1.4932</TD><TD>1.5667</TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,13.82,2,,27.64,2,,41.46,2,,55.28,2,,69.1,2,,82.92,2,,97.54,2,,94.16,2,,90.04,2,,85.32,2,,80.22,2,,73.75,2,"></form> <form><p class=h3><A name="a9"></A>KHCO<sub>3</sub></P> Potassium Bicarbonate, KHCO<sub>3</sub>, Mw=100.1g/M; <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>%(w/w)</TD><TD>50ml</TD><TD>100ml</TD><TD>250ml</TD><TD align="center"><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>KHCO<sub>3</sub></TD><TD>1M</TD><TD>100.1g/M</TD><TD>9.414</TD><TD>5.005g</TD><TD>10.01g</TD><TD>25.03g</TD><TD align="center"><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>H<sub>2</sub>O</TD><TD> </TD><TD>mQ</TD><TD>90.586</TD><TD>48.16ml</TD><TD>96.32ml</TD><TD>240.80ml</TD><TD align="center"><input type="text" size=7 onChange='clc(this,2)'></TD></TR> </TABLE></TD></TR> </TABLE> 1M; p=1.0633, 15<SUP>o</SUP>C<input type="hidden" name="prm" value="100,2,ml,10.01,2,g,96.32,2,ml"></form> <form><p class=h3><A name="a10"></A>Na<sub>2</sub>CO<sub>3</sub></P> Sodium Carbonate, Na<sub>2</sub>CO<sub>3</sub>, Mw=106.0g/M; <br> <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>%(w/w)</TD><TD>50ml</TD><TD>100ml</TD><TD>250ml</TD><TD align="center"><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>Na<sub>2</sub>CO<sub>3</sub></TD><TD>1M</TD><TD>106.0g/M</TD><TD>9.646</TD><TD>5.30g</TD><TD>10.60g</TD><TD>26.50g</TD><TD align="center"><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>H<sub>2</sub>O</TD><TD> </TD><TD>mQ</TD><TD>90.354</TD><TD>49.645ml</TD><TD>99.29ml</TD><TD>248.23ml</TD><TD align="center"><input type="text" size=7 onChange='clc(this,2)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,10.60,2,g,99.29,2,ml"></form> <form><TABLE width="400" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD colspan="2" align="center">1000ml/<input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR align=center bgcolor="eeeeee"><TD>106.0g/M</TD><TD>1M</TD><TD>2M</TD></TR> <TR align=center bgcolor="ffffff"><TD>Na<sub>2</sub>CO<sub>3</sub> [g]</TD><TD>106<br><input type="text" size=6 onChange='clc(this,1)'></TD><TD>212<br><input type="text" size=6 onChange='clc(this,2)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>H<sub>2</sub>O [ml]</TD><TD>992.9<br><input type="text" size=6 onChange='clc(this,3)'></TD><TD>979.2<br><input type="text" size=6 onChange='clc(this,4)'></TD></TR> <TR align=center bgcolor="ffffff"><TD>%(w/w)</TD><TD>9.646</TD><TD>17.797</TD></TR> <TR align=center bgcolor="ffffff"><TD>H<sub>2</sub>O %(w/w)</TD><TD>90.354</TD><TD>82.203</TD></TR> <TR align=center bgcolor="ffffff"><TD>p (g/ml)</TD><TD>1.0989</TD><TD>1.1912</TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,10.6,2,,21.2,2,,99.29,2,,97.92,2,"></form> <form><p class=h3><A name="a11"></A>NaHCO<SUB>3</SUB></P> Sodium Bicarbonate, NaHCO<sub>3</sub>, Mw=84.01g/M; (store at NT).<br> <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR align=center bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR align="center" bgcolor="eeeeee"><td><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><td>%(w/w)</td><TD>50ml</TD><TD>100ml</TD><TD>250ml</TD><td><input type="text" size=7 onCh