Archived Protocol

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> </noscript>  </td></tr> <tr><td width="390" bgcolor="eeeeee" valign="top"> <script language="JavaScript" src=http://molbiol.ru/eng/scripts/sol_a.js type="text/javascript"></script> <div class="inav"> <A href=http://molbiol.ru/eng/protocol/01_07.html#a1>TE</A> 1x, 10x<br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a2>STE</A> 1x, 10x<br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a3>SSC</A> 20x<br> Electrophoresis buffers.<br> <div class="ls"><A href=http://molbiol.ru/eng/protocol/01_07.html#a4>TAE</A> 50x<br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a5>TBE</A> 10x<br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a6>FGRB</A> 50x<br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a7>GTGB</A> 20x<br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a8>TGB</A> 5x<br></div> <A href=http://molbiol.ru/eng/protocol/01_07.html#a9>Loading buffer</A> 10x<br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a10>PEG-NaCl</A><br> Bacteriophage lambda buffers<br> <div class="ls"><A href=http://molbiol.ru/eng/protocol/01_07.html#a11>SM</A><br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a12>STM</A><br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a13>TM</A><br></div> <A href=http://molbiol.ru/eng/protocol/01_07.html#a14>Denaturation buffer</A><br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a15>Neutraliszation buffer</A><br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a16>Developer Rentgen-2</A><br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a17>Fixer BKF-2</A><br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a18>Scintillation solution</A><br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a19>Plasmid solution I</A><br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a20>Plasmid solution II</A><br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a21>Oligonucleotide gel staining mix</A><br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a22>2M Mg<SUP>2+</SUP> solution</A><br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a24>Hybridization buffer HSB (f<SUP>+</SUP>)</A><br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a25>Restriction buffers</A><br> <div class="ls">À., B., L., M., H.<br></div> <A href=http://molbiol.ru/eng/protocol/01_07.html#a26>Denhardt solution</A> 50õ<br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a27>PBS</A> 10x<br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a28>SSPE 20x</A><br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a29>RNase</A> (DNase free)<br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a30></A><br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a31>Proteinase K solution</A><br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a32>EDTA/glycogen solution</A> 20x<br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a33>DNase RQ1 buffer</A><br> <A href=http://molbiol.ru/eng/protocol/01_07.html#a34></A><br> <A href=http://molbiol.ru/eng/protocol/01_07.html#add><img src=http://molbiol.ru/izo/k.gif width="11" height="9" alt="" border="0"> Supplement.</A><br> </div> </TD><td valign="top"> <div class="epig"></div> <br><br><br><br><br><br><br><br> <ul><form name="hlp" id="hlp"><center>About the recalculation of recipes for the arbitrary volume:<br><br><INPUT TYPE="button" VALUE="Help" onClick="helpw()"></center></form></ul> </td></tr> <tr><td width=610 colspan="2" valign="top"> </td></tr></table> <table width="610" border="0" cellspacing="0" cellpadding="0"><tr><td> <script language="JavaScript" src=http://molbiol.ru/scripts/solution.js type="text/javascript"></script> <div class="prot"><ul type="disc"> <form><A name="a1"></A><p class=h3>TE</P> <P>1x; pH7.4; 7.6; 8.0; (store at 4<SUP>o</SUP>C).</P> <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>1ml</TD><TD>5ml</TD><TD>10ml</TD><TD>50ml</TD><TD>400ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>Tris Cl</TD><TD>10mM</TD><TD>1M</TD><TD>10µl</TD><TD>50µl</TD><TD>100µl</TD><TD>500µl</TD><TD>4ml</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>EDTA</TD><TD>1mM</TD><TD>0.5M</TD><TD>2µl</TD><TD>10µl</TD><TD>20µl</TD><TD>100µl</TD><TD>800µl</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD>988µl</TD><TD>4.94ml</TD><TD>9.88ml</TD><TD>49.4ml</TD><TD>395ml</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,1,3,ml,0.2,3,ml,98.8,2,ml"></form> <form><P>10x:</P> <TABLE width="400" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>1ml</TD><TD>5ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>Tris Cl</TD><TD>0.1M</TD><TD>1M</TD><TD>0.1ml</TD><TD>0.5ml</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>EDTA</TD><TD>10mM</TD><TD>0.5M</TD><TD>20µl</TD><TD>0.1ml</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD>mQ</TD><TD> </TD><TD>880µl</TD><TD>4.4ml</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> </TABLE></TD></TR> </TABLE> <li>autoclave sterilization</li><input type="hidden" name="prm" value="100,2,ml,10,2,ml,2,2,ml,88,2,ml"></form> <form><A name="a2"></A><p class=h3>STE</P> <P>1x; (store at 4<SUP>o</SUP>C).</P> <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>1ml</TD><TD>5ml</TD><TD>10ml</TD><TD>50ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>NaCl</TD><TD>0.1M</TD><TD>5M</TD><TD>20µl</TD><TD>100µl</TD><TD>200µl</TD><TD>1.0ml</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>Tris Cl, pH8.0</TD><TD>10mM</TD><TD>1M</TD><TD>10µl</TD><TD>50µl</TD><TD>100µl</TD><TD>0.5ml</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>EDTA, pH8.0</TD><TD>1mM</TD><TD>0.5M</TD><TD>2µl</TD><TD>10µl</TD><TD>20µl</TD><TD>100µl</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD>0.968ml</TD><TD>4.84ml</TD><TD>9.68ml</TD><TD>48.4ml</TD><TD><input type="text" size=7 onChange='clc(this,4)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,2,2,ml,1,2,ml,200,2,µl,96.8,2,ml"></form> <form><P>10x:</P> <TABLE width="400" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>1ml</TD><TD>5ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>NaCl</TD><TD>1.0M</TD><TD>5M</TD><TD>0.2ml</TD><TD>1.0ml</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>Tris Cl</TD><TD>0.1M</TD><TD>1M</TD><TD>0.1ml</TD><TD>0.5ml</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>EDTA</TD><TD>10mM</TD><TD>0.5mM</TD><TD>20µl</TD><TD>0.1ml</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD>mQ</TD><TD> </TD><TD>680µl</TD><TD>3.4ml</TD><TD><input type="text" size=7 onChange='clc(this,4)'></TD></TR> </TABLE></TD></TR> </TABLE> <li>autoclave sterilization</li><input type="hidden" name="prm" value="100,2,ml,20,2,ml,10,2,ml,2,2,ml,68,2,ml"></form> <form><A name="a3"></A><p class=h3>SSC</P> <P>20x; pH 7.0; (store at NT).</P> <TABLE width="500" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>0.5L</TD><TD>1.5L</TD><TD>2L</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>NaCl</TD><TD>3M</TD><TD>58.44g/M</TD><TD>87.66g</TD><TD>263.0g</TD><TD>350.6g</TD><td><input type="text" size=7 onChange='clc(this,1)'></td></TR> <TR bgcolor="ffffff"><TD>Na<SUB>3</SUB>C<SUB>6</SUB>H<SUB>5</SUB>O<SUB>7</SUB>x 5.5H<SUB>2</SUB>O</TD><TD>0.3M</TD><TD>357.1g/M</TD><TD>53.57g</TD><TD>160.7g</TD><TD>214.3g</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD>440.3ml</TD><TD>1.32L</TD><TD>1.76L</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> </TABLE></TD></TR> </TABLE> <P>p = 1.103</P><input type="hidden" name="prm" value="100,2,ml,17.532,2,g,10.713,2,g,88.055,2,ml"></form> <form><A name="a4"></A><p class=h3>TAE</P> <P>1x pH = 7.6:</P> <P class=ls>Tris-acetate 40mM<br> EDTA 1mM</P> <P>store the large stock at 4<SUP>o</SUP>C, working quantity (~100ml) at NT </P> <TABLE width="570" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>100ml</TD><TD>150ml</TD><TD>200ml</TD><TD>250ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>Tris-base</TD><TD>2M</TD><TD>121.1g/M</TD><TD>24.22g</TD><TD>36.33g</TD><TD>48.44g</TD><TD>60.55g</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>EDTA</TD><TD>0.05M</TD><TD>372.3g/M</TD><TD>1.862g</TD><TD>2.792g</TD><TD>3.723g</TD><TD>4.654g</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>Acetic acid</TD><TD>~1.56M</TD><TD>17.4M</TD><TD><U> ~8.96ml</U><br> ~9.40g</TD><TD><U> ~13.44ml</U><br> ~14.10g</TD><TD><U> ~17.92ml</U><br> ~18.80g</TD><TD><U> ~22.40ml</U><br> ~23.50g</TD><TD>~<input type="text" size=7 onChange='clc(this,3)'><br>~<input type="text" size=7 onChange='clc(this,4)'></TD></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD>~73.30g</TD><TD>~109.95g</TD><TD>~146.60g</TD><TD>~183.25g</TD><td>~<input type="text" size=7 onChange='clc(this,5)'></td></TR> </TABLE></TD></TR> </TABLE> <P>p(50x)=1.0878</P> it is convenient to solubilize Tris and EDTA first, and only then add acid to adjust pH. <input type="hidden" name="prm" value="100,2,ml,24.22,2,g,1.8615,3,g,8.9609,2,ml,9.4,2,g,73.3,2,g"></form> <form><A name="a5"></A><p class=h3>TBE</P> <P>10x, pH ~8.3;(store at NT in clean bottle)</P> <TABLE width="500" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>1x</TD><TD>10x</TD><TD>Stock</TD><TD>1L</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>Tris base</TD><TD>89mM</TD><TD>0.89M</TD><TD>121.14g/M</TD><TD>107.8g</TD><td><input type="text" size=7 onChange='clc(this,1)'></td></TR> <TR bgcolor="ffffff"><TD>Boric acid</TD><TD>89mM</TD><TD>0.89M</TD><TD>61.83g/M</TD><TD>55.03g</TD><td><input type="text" size=7 onChange='clc(this,2)'></td></TR> <TR bgcolor="ffffff"><TD>EDTA, pH 8.0</TD><TD>2mM</TD><TD>20mM</TD><TD>500mM</TD><TD><u>40ml</u><br>43.84g</TD><td><input type="text" size=7 onChange='clc(this,3)'><br><input type="text" size=7 onChange='clc(this,4)'></td></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD> </TD><TD>mQ</TD><TD>863.3ml</TD><td><input type="text" size=7 onChange='clc(this,5)'></td></TR> </TABLE></TD></TR> </TABLE> <li>10x stock solution is stable at NT if to clean it through 0.4µm filter;</li> <li>for PAAG it is used as 1x, for agarose gel - as 0.5x;</li> <li>for agarose gels it is used for separation of small (< 200bp) fragments. Generally accepted, that the purification of fragments is more complex from TBE gels.</li> <input type="hidden" name="prm" value="100,2,ml,10.7815,2,g,5.5029,2,g,4,2,ml,4.384,2,g,86.33,2,ml"></form> <form><A name="a6"></A><p class=h3>FGRB</P> <P>50x (formaldehyde gel-running buffer), p=1.104g/ml (store at +4<SUP>o</SUP>C):</P> <TABLE width="583" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>0.5L</TD><TD>1.0L</TD><TD>2.0L</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>MOPS (pH7.0)</TD><TD>1.0M</TD><TD>209.27g/M</TD><TD>104.64g</TD><TD>209.27g</TD><TD>418.54g</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>AcONax3H<SUB>2</SUB>O</TD><TD>0.5M</TD><TD>136.08g/M</TD><TD>34.02g</TD><TD>68.04g</TD><TD>136.08g</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>EDTAx2H<SUB>2</SUB>O</TD><TD>50mM</TD><TD>372.3g/M</TD><TD>9.31g</TD><TD>18.62g</TD><TD>37.23g</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD>404.0ml</TD><TD>808ml</TD><TD>1616ml</TD><TD><input type="text" size=7 onChange='clc(this,4)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,20.927,2,g,6.804,2,g,1.8615,2,g,80.8,2,ml"></form> <form><TABLE width="583" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>0.5L</TD><TD>1.0L</TD><TD>2.0L</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>MOPS (pH7.0)</TD><TD>1.0M</TD><TD>209.27g/M</TD><TD>104.64g</TD><TD>209.27g</TD><TD>418.54g</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>AcONa anhydrous</TD><TD>0.5M</TD><TD>82.04g/M</TD><TD>20.51g</TD><TD>41.02g</TD><TD>82.04g</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>EDTA x 2H<SUB>2</SUB>O</TD><TD>50mM</TD><TD>372.3g/M</TD><TD>9.31g</TD><TD>18.62g</TD><TD>37.23g</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD>417.5ml</TD><TD>835ml</TD><TD>1670ml</TD><TD><input type="text" size=7 onChange='clc(this,4)'></TD></TR> </TABLE></TD></TR> </TABLE> <li>adjust pH7.0 10N NaOH (per 500ml of buffer ~22ml), filter-sterilize through 0.22µm filter;</li> <li>solution becomes yellow during the storage on the light, but it has no effect on the solution quality.</li> <input type="hidden" name="prm" value="100,2,ml,20.927,2,g,4.102,2,g,1.8615,2,g,83.5,2,ml"></form> <form><A name="a7"></A><p class=h3>GTGB</P> <P>20x /Glycerol tolerant gel buffer/ (store at +4<SUP>o</SUP>C):</P> <TABLE width="450" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>1L</TD><TD>1.5L</TD><TD>2.0L</TD><TD>2.5L</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD><P>Tris base</TD><TD>1.78M</TD><TD>121.14g/M</TD><TD>216g</TD><TD>324g</TD><TD>432g</TD><TD>540g</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD><P>Taurine</TD><TD>0.56M</TD><TD>125.15g/M</TD><TD>72g</TD><TD>108g</TD><TD>144g</TD><TD>180g</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD><P>Na<SUB>2</SUB>EDTAx2H<SUB>2</SUB>O</TD><TD>10.7mM</TD><TD>372.3 g/M</TD><TD>4g</TD><TD>6g</TD><TD>8g</TD><TD>10g</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> <TR bgcolor="ffffff"><TD><P>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD>802ml</TD><TD>1203ml</TD><TD>1604ml</TD><TD>2005ml</TD><TD><input type="text" size=7 onChange='clc(this,4)'></TD></TR> </TABLE></TD></TR> </TABLE> <P>p=1.094</P> <li>Taurine: C<sub>2</sub>H<sub>7</sub>NO<sub>3</sub>S; Mw=125.15g/M.</li><input type="hidden" name="prm" value="100,2,ml,21.563,2,g,7.2,2,g,0.4,3,g,80.2,2,ml"></form> <form><A name="a8"></A><p class=h3>TGB</P> <P>Tris-Glycine buffer 5x, pH8.3, (store the main stock at +4<SUP>o</SUP>C, working quantity at NT).</P> <TABLE width="565" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>1x</TD><TD>Conc.</TD><TD>Stock</TD><TD>1L</TD><TD>1.5L</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>Tris-base</TD><TD>25mM</TD><TD>125mM</TD><TD>121.1g/M</TD><TD>15.1g</TD><TD>22.71g</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>Glycine</TD><TD>250mM</TD><TD>1.25M</TD><TD>75.05g/M</TD><TD>94g</TD><TD>141g</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>SDS</TD><TD>0.1%</TD><TD>0.5%</TD><TD>10%</TD><TD>50ml</TD><TD>75ml</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD> </TD><TD>mQ</TD><TD> </TD><TD> </TD><TD> </TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,1.51425,2,g,9.3813,2,g,5,2,ml"></form> <form><A name="a9"></A><p class=h3>Loading buffer</P> <P>10x, (store at NT).</P> <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>1ml</TD><TD>5ml</TD><TD>10ml</TD><TD>25ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>Xilene cyanol</TD><TD>0.1%</TD><TD>solid</TD><TD>1.0mg</TD><TD>5.0mg</TD><TD>10mg</TD><TD>25.0mg</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>Bromph. blue</TD><TD>0.1%</TD><TD>solid</TD><TD>1.0mg</TD><TD>5.0mg</TD><TD>10mg</TD><TD>25.0mg</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>SDS</TD><TD>0.5%</TD><TD>10%</TD><TD>50µl</TD><TD>250µl</TD><TD>500µl</TD><TD>1.25ml</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> <TR bgcolor="ffffff"><TD>EDTA, pH8.0</TD><TD>0.1M</TD><TD>0.5M</TD><TD>0.2ml</TD><TD>1.0ml</TD><TD>2.0ml</TD><TD>5.0ml</TD><TD><input type="text" size=7 onChange='clc(this,4)'></TD></TR> <TR bgcolor="ffffff"><TD>Glycerol</TD><TD>50%</TD><TD>100%</TD><TD><U> 0.5ml</U><br> 0.625g</TD><TD><U> 2.5ml</U><br> 3.125g</TD><TD><U> 5.0ml</U><br> 6.25g</TD><TD><U> 12.5ml</U><br> 15.63g</TD><TD><input type="text" size=7 onChange='clc(this,5)'><br><input type="text" size=7 onChange='clc(this,6)'></TD></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD>0.25ml</TD><TD>1.25ml</TD><TD>2.5ml</TD><TD>6.25ml</TD><TD><input type="text" size=7 onChange='clc(this,7)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,100,2,mg,100,2,mg,5,2,ml,20,2,ml,50,2,ml,62.5,2,g,25,2,ml"></form> <form><A name="a10"></A><p class=h3>PEG-NaCl</P> <P>(store at 4<SUP>o</SUP>C).</P> <TABLE width="500" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>50ml</TD><TD>100ml</TD><TD>150ml</TD><TD>200ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>NaCl</TD><TD>1.6M</TD><TD>58.44g/M</TD><TD>4.675g</TD><TD>9.35g</TD><TD>14.03g</TD><TD>18.70g</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>PEG-6000</TD><TD>13%</TD><TD>solid</TD><TD>6.50g</TD><TD>13.00g</TD><TD>19.50g</TD><TD>26.00g</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,9.3504,2,g,13,2,g"></form> <form><P>or:</P> <TABLE width="500" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>50ml</TD><TD>100ml</TD><TD>150ml</TD><TD>200ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>NaCl</TD><TD>1.6M</TD><TD>5M</TD><TD><U> 16ml</U><br> 18.97g</TD><TD><U> 32ml</U><br> 37.95g</TD><TD><U> 48ml</U><br> 56.92g</TD><TD><U> 64ml</U><br> 75.90g</TD><TD><input type="text" size=7 onChange='clc(this,1)'><br> <input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>PEG-6000</TD><TD>13%</TD><TD>40%</TD><TD>16.25ml</TD><TD>32.50ml</TD><TD>48.75ml</TD><TD>65.00ml</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,32,2,ml,37.95,2,g,32.5,2,ml"></form> <form><A name="a11"></A><p class=h3>SM</P> <P>(store at 4<SUP>o</SUP>C).</P> <TABLE width="500" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>10ml</TD><TD>50ml</TD><TD>100ml</TD><TD>150ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>NaCl</TD><TD>0.1M</TD><TD>5M</TD><TD>200µl</TD><TD>1.0ml</TD><TD>2.0ml</TD><TD>3.0ml</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>MgSO<SUB>4</SUB>*</TD><TD>10mM</TD><TD>1M</TD><TD>100µl</TD><TD>0.5ml</TD><TD>1.0ml</TD><TD>1.5ml</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>Tris Cl, pH7.5</TD><TD>50mM</TD><TD>1M</TD><TD>0.5ml</TD><TD>2.5ml</TD><TD>5.0ml</TD><TD>7.5ml</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> <TR bgcolor="ffffff"><TD></TD><TD>0.01%</TD><TD>2%</TD><TD>50µl</TD><TD>0.25ml</TD><TD>0.5ml</TD><TD>0.75ml</TD><TD><input type="text" size=7 onChange='clc(this,4)'></TD></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD>9.15ml</TD><TD>45.75ml</TD><TD>91.5ml</TD><TD>137.25ml</TD><TD><input type="text" size=7 onChange='clc(this,5)'></TD></TR> </TABLE></TD></TR> </TABLE> <P>* - add after autoclaving; </P> <li>autoclave sterilization</li><input type="hidden" name="prm" value="100,2,ml,2,2,ml,1,2,ml,5,2,ml,0.5,2,ml,91.5,2,ml"></form> <form><A name="a12"></A><p class=h3>STM</P> <P>(store at 4<SUP>o</SUP>C):</P> <TABLE width="400" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>50ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>NaCl</TD><TD>10mM</TD><TD>5M</TD><TD>100µl</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>Tris Cl pH8.0</TD><TD>50mM</TD><TD>1M</TD><TD>2.5ml</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>MgCl<SUB>2</SUB></TD><TD>10mM</TD><TD>1M</TD><TD>500µl</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD>46.9ml</TD><TD><input type="text" size=7 onChange='clc(this,4)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,200,2,µl,5,2,ml,1,2,ml,93.8,2,ml"></form> <form><p class=h3><A name="a13"></A>TM</P> <P>(store at 4<SUP>o</SUP>C):</P> <TABLE width="400" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>50ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>Tris Cl pH8.0</TD><TD>50mM</TD><TD>1M</TD><TD>2.5ml</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>MgCl<SUB>2</SUB></TD><TD>10mM</TD><TD>1M</TD><TD>500µl</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD>47.0ml</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,5,2,ml,1,2,ml,94,2,ml"></form> <form><A name="a14"></A><p class=h3>Denaturation buffer</P> <P>(store at NT).</P> <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>100ml</TD><TD>150ml</TD><TD>300ml</TD><TD>500ml</TD><TD>750ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>NaCl</TD><TD>1.5M</TD><TD>58.44g/M</TD><TD>8.766g</TD><TD>13.15g</TD><TD>26.30g</TD><TD>43.83g</TD><TD>65.75g</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>NaOH</TD><TD>0.5M</TD><TD>10M</TD><TD><U> 5ml</U><br> 6.65g</TD><TD><U> 7.5ml</U><br> 9.97g</TD><TD><U> 15ml</U><br> 19.94g</TD><TD><U> 25ml</U><br> 33.23g</TD><TD><U> 37.5ml</U><br> 49.84g</TD><TD><input type="text" size=7 onChange='clc(this,2)'><br> <input type="text" size=7 onChange='clc(this,3)'></TD></TR> <TR bgcolor="ffffff"><TD> </TD><TD> </TD><TD>40g/M</TD><TD>2g</TD><TD>3g</TD><TD>6g</TD><TD>10g</TD><TD>15g</TD><TD><input type="text" size=7 onChange='clc(this,4)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,8.766,2,g,5,2,ml,6.65,2,ml,2,2,g"></form> <form><A name="a15"></A><p class=h3>Neutralization buffer</P> <P>(store at NT).</P> <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>150ml</TD><TD>300ml</TD><TD>500ml</TD><TD>750ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>Tris Cl, pH7.4</TD><TD>1M</TD><TD>121.1g/M</TD><TD>18.15g</TD><TD>36.3g</TD><TD>60.5g</TD><TD>90.75g</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>NaCl</TD><TD>1.5M</TD><TD>58.44g/M</TD><TD>13.15g</TD><TD>26.30g</TD><TD>43.83g</TD><TD>65.75g</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>HCl</TD><TD>~0.81M</TD><TD>11.6M/L</TD><TD>~10.5ml</TD><TD>~21ml</TD><TD>~35ml</TD><TD>~52.5ml</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> <TR bgcolor="ffffff"><TD>EDTA (optional)</TD><TD>1mM</TD><TD>0.5M</TD><TD>0.3ml</TD><TD>0.6ml</TD><TD>1ml</TD><TD>1.5ml</TD><TD><input type="text" size=7 onChange='clc(this,4)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,12.11,2,g,8.766,2,g,7,2,ml,0.2,2,ml"></form> <form><A name="a16"></A><p class=h3>Developer Rentgen-2</P> <P>(store at NT in the dark).</P> <TABLE width="400" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>500ml</TD><TD>800ml</TD><TD>1l</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD></TD><TD>1.1g</TD><TD>1.76g</TD><TD>2.2g</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>Na<SUB>2</SUB>SO<SUB>3</SUB> anhidr.</TD><TD>36.0g</TD><TD>57.6g</TD><TD>72.0g</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>Hydroquinone</TD><TD>4.4g</TD><TD>7.04g</TD><TD>8.8g</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> <TR bgcolor="ffffff"><TD>Na<sub>2</sub>CO<sub>3</sub></TD><TD>24.0g</TD><TD>38.4g</TD><TD>48.0g</TD><TD><input type="text" size=7 onChange='clc(this,4)'></TD></TR> <TR bgcolor="ffffff"><TD>KBr</TD><TD>2.0g</TD><TD>3.2g</TD><TD>4.0g</TD><TD><input type="text" size=7 onChange='clc(this,5)'></TD></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD>490ml</TD><TD>784ml</TD><TD>980ml</TD><TD><input type="text" size=7 onChange='clc(this,6)'></TD></TR> </TABLE></TD></TR> </TABLE><P> <P>solubilize in the presented order , p=1.115g/ml.</P><input type="hidden" name="prm" value="100,2,ml,0.22,2,g,7.2,2,g,0.88,2,g,4.8,2,g,0.4,2,g,98,2,ml"></form> <form><A name="a17"></A><p class=h3>Fixer BKF-2</P> <P>(store at NT).</P> <TABLE width="450" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>500ml</TD><TD>800ml</TD><TD>1l</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>Na<SUB>2</SUB>S<SUB>2</SUB>O<SUB>3</SUB>x 5H<SUB>2</SUB>O</TD><TD>130g</TD><TD>208g</TD><TD>260g</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>NH<SUB>4</SUB>Cl</TD><TD>25g</TD><TD>40g</TD><TD>50g</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>Sodium pyrosulfite</TD><TD>8.5g</TD><TD>13.6g</TD><TD>17g</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD>398ml</TD><TD>636.8ml</TD><TD>796ml</TD><TD><input type="text" size=7 onChange='clc(this,4)'></TD></TR> </TABLE></TD></TR> </TABLE> <P>solubilize in the presented order, p=1.123g/ml.</P><input type="hidden" name="prm" value="100,2,ml,26,2,g,5,2,g,1.7,2,g,79.6,2,ml"></form> <form><A name="a18"></A><p class=h3>Scintillation solution</P> <P>(store at NT in the dark):</P> <TABLE width="400" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>0.5L</TD><TD>1L</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>PPO</TD><TD>2.50g</TD><TD>5.00g</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>POPOP</TD><TD>0.05g</TD><TD>0.10g</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>Toluene</TD><TD> </TD><TD> </TD><td> </td></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,0.5,2,g,0.01,3,g"></form> <form><A name="a19"></A><p class=h3>Plasmid solution I</P> <P>(store at +4<SUP>o</SUP>C)</P> <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>50ml</TD><TD>100ml</TD><TD>150ml</TD><TD>200ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>Glucose</TD><TD>50mM</TD><TD>2M</TD><TD><U> 1.25ml</U><br> 1.41g</TD><TD><U> 2.5ml</U><br> 2.825g</TD><TD><U> 3.75ml</U><br> 4.24g</TD><TD><U> 5.0ml</U><br> 5.56g</TD><TD><input type="text" size=7 onChange='clc(this,1)'><br><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>Tris Cl, pH 8.0</TD><TD>25mM</TD><TD>1.0 M</TD><TD>1.25ml</TD><TD>2.5ml</TD><TD>3.75ml</TD><TD>5.0ml</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> <TR bgcolor="ffffff"><TD>EDTA</TD><TD>10mM</TD><TD>0.5 M</TD><TD>1.0ml</TD><TD>2.0ml</TD><TD>3.0ml</TD><TD>4.0ml</TD><TD><input type="text" size=7 onChange='clc(this,4)'></TD></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD>46.5ml</TD><TD>93ml</TD><TD>139.5ml</TD><TD>186ml</TD><TD><input type="text" size=7 onChange='clc(this,5)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,2.5,2,ml,2.825,2,g,2.5,2,ml,2,2,ml,93,2,ml"></form> <form><A name="a20"></A><p class=h3>Plasmid solution II</P> <TABLE width="550" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>0.5ml</TD><TD>1ml</TD><TD>2.5ml</TD><TD>5.0ml</TD><TD>6.5ml</TD><TD>8.5ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>NaOH</TD><TD>0.2N</TD><TD>1N</TD><TD>0.1ml</TD><TD>0.2ml</TD><TD>0.5ml</TD><TD>1.0ml</TD><TD>1.3ml</TD><TD>1.7ml</TD><TD><input type="text" size=7 onChange='clc(this,1)'></TD></TR> <TR bgcolor="ffffff"><TD>SDS</TD><TD>1%</TD><TD>10%</TD><TD>0.05ml</TD><TD>0.1ml</TD><TD>0.25ml</TD><TD>0.5ml</TD><TD>0.65ml</TD><TD>0.85ml</TD><TD><input type="text" size=7 onChange='clc(this,2)'></TD></TR> <TR bgcolor="ffffff"><TD>H<SUB>2</SUB>O</TD><TD> </TD><TD>mQ</TD><TD>0.350ml</TD><TD>0.7ml</TD><TD>1.75ml</TD><TD>3.5ml</TD><TD>4.55ml</TD><TD>5.95ml</TD><TD><input type="text" size=7 onChange='clc(this,3)'></TD></TR> </TABLE></TD></TR> </TABLE><input type="hidden" name="prm" value="100,2,ml,20,2,ml,10,2,ml,70,2,ml"></form> <form><p class=h3><A name="a21"></A>Oligonucleotide gel staining mix</P> <P>(store at NT).</P> <TABLE width="450" border="0" cellspacing="0" cellpadding="0"> <TR bgcolor="000000"> <TD><TABLE width="100%" border="0" cellspacing="1" cellpadding="2px"> <TR bgcolor="eeeeee"><td align="center"><input type="Reset" value='Clear'></td><TD>Conc.</TD><TD>Stock</TD><TD>100ml</TD><TD>200ml</TD><TD>300ml</TD><TD>500ml</TD><TD><input type="text" size=7 onChange='clc(this,0)'></TD></TR> <TR bgcolor="ffffff"><TD>AcONa(5.2)</TD><TD>0.5M</TD><TD>3M</TD><TD>17ml</TD><TD>33ml</TD><TD>50.0ml</TD><TD>83ml</TD><TD><input type="tex