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Microscopy Sample PreparationTechnical Information from Energy Beam Sciences
  EBSciences  

Applications, Techniques, and Protocols

Immunolabeling
Microwave Applications, Techniques and Procedures
Microtomy Techniques
SEM Operation
SEM Sample Preparation
Spurr Epoxy Sections
Plastic Embedded Tissue


Immunolabeling

Immunogold Labeling with BioSite Colloidal Gold Reagents
BioSite Cationic Colloidal Gold
Immunogold Labelling in Scanning Electron Microscopy


Microwave Applications, Techniques and Procedures

Special Stains Using the Microwave
Joyce Moore of Jefferson Regional Medical Center, Pine Bluff AR

Microwave Processing Techniques for Microscopy
Energy Beam Sciences, Inc.

References for Microwave Polymerization of EM Resins

Microwave Antigen Retrieval Technique
Shi, Shan-Rong et al., "Antigen retrieval technique: A novel approach to immunohistochemistry on routinely processed tissue sections," , in Gu, J, ed. Analytical Morphology: Theory, Applications and Protocols, BioTechniques Books, div. of Eaton Publishing, 1997.

Microwave Irradiation of Cryostat Sections Accelerates and Improves Nitric Oxide Synthase Staining,
Michele Forte and Jiang Gu, Cell Vision, Vol. 1, No. 1, 1994. posted with permission.

Working Procedures in Microwave Histology,
Vincent R. Klump, Jr., HT (ASCP), Histology Services, East Haven, CT

Microwave Tissue Processing
From Donna Willis, Harris Methodist Hospital, Ft. Worth, Texas

Microwave Oven Quality Control: Measuring Output
From Donna Willis, Harris Methodist Hospital, Ft. Worth, Texas

Microwave Biopsy Histoprocessing Procedure III
From Denise Hardy, ARUP Laboratories, Salt Lake City, UT

Accumate ™ RDO Rapid bone Decalcification Procedure

Fixation in the MicroWave

Microwave Stimulated Fixation With Preserve™


Microtomy Techniques

VibratomeŽ Operation
Application Note from Energy Beam Sciences


SEM Operation

Using SG Pointed Filaments
Application Note from Energy Beam Sciences


SEM Sample Preparation

High Resolution Coatings for Field Emission SEM
Application Note from Energy Beam Sciences

Plasma Chemistry: Applications of Barrel Reactors
Application Note from Energy Beam Sciences

Plasma Chemistry Applied to Electron Microscopy (EM) Preparation Procedures
Application Note from Energy Beam Sciences

Carbon Coating Thickness
Dr. Norman Charnley, Department of Earth Sciences, University of Oxford

Spurr Epoxy Sections

Diagnostic Electron Microscopy on Reembedded ("Popped Off") Areas of Large Spurr Epoxy Sections
P. Anthony di Sant'Agnese and Karen L. De Mesy-Jensen

Large Block Embedding and "Pop-Off" Technique for Immunoelectron Microscopy
Karen L. De Mesy-Jensen and by P. Anthony di Sant'Agnese


Plastic Embedded Tissue

Staining Procedures For Plastic Embedded Tissue
Verified at the Applications Laboratory of the Biomedical Division, Sorvall Microtomes. 

Oil Red O Stain
Bob Schoonhoven, University of North Carolina

Alkaline Phosphatase for Osteoblasts
Patsy Ruegg, Metabolic Bone Disease Laboratory of the University of Colorado Health Sciences Center

Acid Phosphatase

Glycol Methacrylate: Embedding and Staining

Technovit® 7100 and 8100 Application Information from Heraeus Kulzer


Methyl Methacrylate: Embedding and Staining

Technovit® 9100 Application Information from Heraeus Kulzer


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