This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research objectives and tissue under study.
TIP: Tissue processors in standard histopathology laboratories generally include formalin-fixation as the first two steps in the paraffin infiltration procedure. Make certain these steps are avoided when processing tissue intended for molecular profiling.
Back to Protocols used at NCI