Protocol Online: Cached

This is a cached page for the URL (http://www.ukans.edu/~bcmic/MEIL/techniques/epon.html). To see the most recent version of this page, please click here.

Protocol Online is not affiliated with the authors of this page nor responsible for its content. About Cache

Microscopy Laboratory Techniques

EPON resin mixture for transmission electron microscopy

For Epon WPE 153:

		~120 ml	~60 ml	~30 ml
Mix A:	Embed 812	44 ml	22.1 ml	11.1 ml
Mix A:	DDSA	67 ml	33.3 ml	16.7 ml
Mix B:	Embed 812	67 ml	33.3 ml	16.7 ml
Mix B:	NMA	56 ml	27.8 ml	13.9 ml

Prepare mixture A and mixture B in 30 - 120 ml batches. Mix well and store in glass scintillation vials or some other container that can be capped tightly. Store at -20 degrees C. The mixtures can be stored for years.

For use:

Be certain to completely warm the vials before opening them to prevent water from mixing with the plastic.
Mix equal volumes of A and B together.
Then add 0.1 ml of DMP-30 for each 5 ml of plastic.
Mix well and store no longer than a day at room temp. I usually put the remainder of the plastic in a tube or vial, cap and wrap the cap with parafilm, and put it at 4 degrees C overnight.