EPON resin mixture for transmission electron microscopy For Epon WPE 153:
| ||~120 ml||~60 ml||~30 ml |
|Mix A:||Embed 812||44 ml||22.1 ml||11.1 ml|
|DDSA||67 ml||33.3 ml||16.7 ml|
|Mix B:||Embed 812||67 ml||33.3 ml||16.7 ml|
|NMA||56 ml||27.8 ml||13.9 ml|
Prepare mixture A and mixture B in 30 - 120 ml batches. Mix well and store in glass scintillation vials or some other container that can be capped tightly. Store at -20 degrees C. The mixtures can be stored for years.
- Be certain to completely warm the vials before opening them to prevent water from mixing with the plastic.
- Mix equal volumes of A and B together.
- Then add 0.1 ml of DMP-30 for each 5 ml of plastic.
- Mix well and store no longer than a day at room temp. I usually put the remainder of the plastic in a tube or vial, cap and wrap the cap with parafilm, and put it at 4 degrees C overnight.