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Solutions for Yeast Complete (YC) Media

100X YC (7 Dropout Amino Acid Liquid)

1.0 g arginine
0.5 g aspartic acid
0.5 g isoleucine
0.5 g phenylalanine
0.5 g proline
0.5 g serine
1.0 g threonine
0.5 g valine

in final volume of 100 ml
stir with low heat until into solution
filter sterilze

10X YNB + tyrosine

17 g YNB (w/o amino acids and w/o ammonium sulfate)
50 g ammonium sulfate
0.5 g tyrosine

in a final volume of 1L
stir with low heat until into solution
filter sterilze

Amino Acid Solutions (100X stocks, except URA=10X stock)

URA 1 mg/ml
ADE 1 mg/ml
LYS 10 mg/ml
MET 5 mg/ml
TRP 10 mg/ml
LEU 10 mg/ml
HIS 5 mg/ml

Autoclave to sterilize, except filter sterilize TRP

Store all stocks at 4 deg C, except LEU (store at RT)

Example Recipe of Complete Plates

20 g of Agar in 630 ml distilled water in a 2L flask
Autoclave 20 minutes

Add the following:

100 ml	20% sterile glucose
100 ml	10X YNB solution
10 ml	100X YC-7aa dropout solution
100 ml	URA solution
10 ml	ADE solution
10 ml	LYS solution
10 ml	MET solution
10 ml	TRP solution
10 ml	LEU solution
10 ml	HIS solution

FOA plates

20 g of Agar in 710 ml distilled water in a 2L flask
Autoclave 20 minutes

Add the following:

100 ml	20% sterile glucose
100 ml	10X YNB solution
10 ml	100X YC-7aa dropout solution
20 ml	URA solution
10 ml	ADE solution
10 ml	LYS solution
10 ml	MET solution
10 ml	TRP solution
10 ml	LEU solution
10 ml	HIS solution

Let flask cool to 50 deg C, then add 1.0 g of 5-FOA.

Prepared By:
Michael McMurray
(206) 667-6660
Last Modified: Aug 29, 2001