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Solutions for Yeast Complete (YC) Media


  1. 100X YC (7 amino acid dropout solution)
  2. 10X YNB + tyrosine solution
  3. Amino acid solutions
  4. YC plate recipe
  5. FOA plate recipe

100X YC (7 Dropout Amino Acid Liquid)

1.0 g arginine
0.5 g aspartic acid
0.5 g isoleucine
0.5 g phenylalanine
0.5 g proline
0.5 g serine
1.0 g threonine
0.5 g valine

in final volume of 100 ml
stir with low heat until into solution
filter sterilze


10X YNB + tyrosine

17 g YNB (w/o amino acids and w/o ammonium sulfate)
50 g ammonium sulfate
0.5 g tyrosine

in a final volume of 1L
stir with low heat until into solution
filter sterilze


Amino Acid Solutions (100X stocks, except URA=10X stock)

URA 1 mg/ml
ADE 1 mg/ml
LYS 10 mg/ml
MET 5 mg/ml
TRP 10 mg/ml
LEU 10 mg/ml
HIS 5 mg/ml

Autoclave to sterilize, except filter sterilize TRP

Store all stocks at 4 deg C, except LEU (store at RT)


Example Recipe of Complete Plates

20 g of Agar in 630 ml distilled water in a 2L flask
Autoclave 20 minutes

Add the following:
100 ml 20% sterile glucose
100 ml 10X YNB solution
10 ml 100X YC-7aa dropout solution
100 ml URA solution
10 ml ADE solution
10 ml LYS solution
10 ml MET solution
10 ml TRP solution
10 ml LEU solution
10 ml HIS solution


FOA plates

20 g of Agar in 710 ml distilled water in a 2L flask
Autoclave 20 minutes

Add the following:
100 ml 20% sterile glucose
100 ml 10X YNB solution
10 ml 100X YC-7aa dropout solution
20 ml URA solution
10 ml ADE solution
10 ml LYS solution
10 ml MET solution
10 ml TRP solution
10 ml LEU solution
10 ml HIS solution

Let flask cool to 50 deg C, then add 1.0 g of 5-FOA.

 
Prepared By:
Michael McMurray
(206) 667-6660
Last Modified: Aug 29, 2001


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