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Yeast Protocols

Solutions for Hartwell's Complete (HC) Media

2.4 g tyr
3.2 g ile
2.0 g phe
4.0 g glu
8.0 g thr
4.0 g asp
6.0 g val
16.0 g ser
0.8 g arg

in final volume of 4L - Autoclave

58 g YNB (w/o amino acids and w/o ammonium sulfate)
200 g ammonium sulfate

in a final volume of 4L - Autoclave

URA 1 mg/ml
ADE 1 mg/ml
LYS 10 mg/ml
MET 2 mg/ml
TRP 10 mg/ml
LEU 20 mg/ml
HIS 10 mg/ml

20 g of Agar in 609 ml distilled water in a 2L flask
Autoclave 20 minutes

Add the following:

20 g of Agar in 609 ml distilled water in a 2L flask
Autoclave 20 minutes

Add the following:

Let flask cool to 50 deg C, then add 1.0 g of 5-FOA.

Prepared By:
Michael McMurray
(206) 667-6660
Last Modified: Aug 29, 2001