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NEBcutter V1.0

NEBcutter V1.0



This tool will take a DNA sequence and find the large, non-overlapping open reading frames and the sites for all restriction enzymes that cut the sequence just once. By default, only enzymes available from NEB are used, but other sets may be chosen. Just enter your sequence and "submit". Further options will appear with the output.
The maximum size of the input file is 1 MByte, and the maximum sequence length is 200 KBases.

Warning: your browser is not fully supported by NEBcutter. To achieve full functionality please use Netscape Navigator 4.0 or higher, or Microsoft Internet Explorer 4.0 or higher.
Local sequence file:
GenBank number:    (Browse GenBank)
or paste in your DNA sequence:   (plain or FASTA format)
The sequence is: Linear
Enzymes to use: NEB enzymes
All commercially available specificities
All specificities
Minimum ORF length to display: a.a.
Name of sequence:    (optional)

Earlier projects:

Note: Your earlier projects will be deleted 2 days after they were last accessed.
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