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Tm Determination

Tm Determination

These calculations are done essentially as described by Breslauer et al., Proc. Nat. Acad. Sci. 83, 3746-50, 1986 (Abstract). This program assumes that the sequences are not symmetric and contain at least one G or C. The minimum length for the query sequence is 8.

Query:

Conc (nM)

Salt (mM)


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Comments, questions, and bug reports to stew@lenti.med.umn.edu

13:40 CDT - 7 Aug 1995