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Breeden Lab, RNA miniprep

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Yeast RNA Miniprep

(All buffer components and stock-solutions, except the Tris-stock-solution, are DEPC treated and autoclaved)

50 mM Tris (HCl) pH 7.4; 100 mM NaCl; 10 mM EDTA
[for 100 ml: 5 ml 1M Tris pH 7.4; 2.5 ml 4 M NaCl; 2 ml 0.5 M EDTA]

RNA-buffer + 1.3 % SDS