This is a cached page for the URL (http://www.umanitoba.ca/faculties/medicine/biochem/gietz/media.html). To see the most recent version of this page, please click here.
Protocol Online is not affiliated with the authors of this page nor responsible for its content.
About Cache
The Yeast Media Page


The YEAST MEDIA PAGE




 

We always use a 1 liter flask and make 600 mls of media because it gives exactly 20 plates (1 Sleeve of plates). In addition this volume of liquid is more easily handled when pouring after autoclaving.

 



SYNTHETIC COMPLETE DROP OUT(SC drop-out)MEDIUM
 

Add the ingredients (except Agar) to the water and mix and adjust the pH to 5.6 with 10 N NaOH. (THIS STEP IS IMPORTANT FOR highest efficiency TRANSFORMATION)
Autoclave at 121°C for 15 min.