This is a cached page for the URL ( To see the most recent version of this page, please click here.
Protocol Online is not affiliated with the authors of this page nor responsible for its content.
About Cache
Non-technical overview

Mutagenizing a yeast gene with a mini-transposon

First, clone gene into vector pHSS6. Then:

  1. Day 1: Transform plasmid into bacteria expressing transposase.
  2. Day 2: Mate transformants to bacteria carrying mTn (Mating means mixing the cells and plating with selection).
  3. Day 3: Scrape off cells and mate to bacteria carrying resolvase.
  4. Day 4: Scrape off cells, make a miniprep and transform into E. coli.
  5. Day 5: Scrape off transformants, make a miniprep, cut with NotI.
  6. Day 6: Transform digest into yeast.

It's just that simple.

YGAC Home page | TRIPLES | The current project | Insertion libraries | Protocols | Reagents | Publications | Project personnel | Protein Microarray | Other Lab Protocols (ChIp-ChIp)