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Breeden Lab, ß-GAL filter assay

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ß - GAL Filter Assay


60 mM Na
2HPO4 (anhyd.); 60 mM NaH2PO4; 10 mM KCl; 1 mM MgSO4
[for 500 ml: 4.26 g Na
2HPO4 (anhyd.); 2.40 g NaH2PO4; 373 mg KCl; 123 mg MgSO4; adjust to pH 7.0]

 X-GAL solution:
40 mg/ml in DMF (dimethylformamide)