This page will provide unique protocols which have been developed in our laboratory. It is by no means a comprehensive listing of all of the protocols in use. Some protocols are available as Adobe Acrobat files. You will need the free Acrobat reader to view these. Follow the button below if you need to download the reader.

For microarray protocols, as well as information about how make your own microarrayer, see the MGuide, available through the Brown Lab website.

Microarray Protocols

Microarray protocols at the Brown Lab webpage

Human Genetics

• Nonradioactive Multiplex Analysis of CA Repeat Markers (M. Gschwend)

Yeast Biology

• Immuno-EM protocol (Jon Mulholland)

• Gene Disruption by Fusion PCR (E. Beasley and D. Amberg)

• Actin Capture Assay (D. Amberg)

• Immunofluorescence (with Methanol) (D. Amberg)

• Immunofluorescence (without methanol) (D. Amberg)

• Rhodamine-Phalloidin/Calcofluor Staining (D. Amberg)

General Techniques

• Vectorette PCR (C. Friddle)