This is a cached page for the URL (http://sequence-www.stanford.edu/group/yeast_deletion_project/Riles_YPDplateprot.html). To see the most recent version of this page, please click here.
Protocol Online is not affiliated with the authors of this page nor responsible for its content.
About Cache
YeastDeletionWebPages
Deletion Page Home

Databases and Datasets

FAQs

Deletion Strains Available

References

Protocols &Technical Information

Useful Sites & Links

Consortium Members' Addresses

Yeast Deletion Database (consortium members only)

 

YPD FILLED MICROTITER PLATES

Uses:
1. Grow individual strains to use for PCR
2. Shipping strains


1.
Make YPD regular agar (1.5-2%) for sending strains.

2. Make 200ml batches = enough for 6 microtiter plates.

3. Add G418 at 200mg/L.

4. Keep the medium at 65° or hotter. Place the multichannel pipettor basin on a block
that is set at 65°. Pour the hot medium nearly to the top.

5. Dispense agar media into wells. Do not blow out the final drop of
medium into the wells (makes bubbles).

Colony PCR: 200µl per well

Prior to inoculating cells, add 10µl water to each well. Then inoculate the
wells with sterile toothpicks. After all the wells are inoculated, give the
plate a gentle swirl to spread the cells on the surface of the medium in
each well. This makes semi-liquid cultures for use as template for
colony PCR, after which the plates can be parafilmed and stored.

Sending strains: 150µl per well

Let plates sit on bench to dry for several days before inoculating wells.
Do NOT add water to the wells.

 

from Linda Riles, November 1997