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Quick Guide Chart for Intracellular Staining:
eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified, FITC, Cy5, PE, PE-Cy5, and APC conjugates. For more information about the properties of fluorescent dyes, please visit our Fluorescent Dyes page in our BestProtocols. The Functional Grade Purified format is recommended for neutralization studies. More antibodies are in development; if you do not see an antibody of your choice, please send us an e-mail so we may provide you timely product updates. | | Cytokine | Source | Activation | Time | Restimulation | Block | Antibody | | | |
mIL-2 |
mouse
spleen | 1) ConA (3µg/ml) for two days 2) IL-2 (20ng/ml) and IL-4 (20ng/ml) for three days
|
2d/3d | anti-CD3 (10µg/ml immobilized) + anti-CD28 (2µg/ml soluble) + monensin for 5hrs |
Monensin |
 JES6-5H4
| | | | | mIL-4 | mouse
spleen
| 1) ConA (3µg/ml) for two days 2) IL-2 (20ng/ml) and IL-4 (20ng/ml) for three days
| 2d/3d | anti-CD3 (10µg/ml immobilized) + anti-CD28 (2µg/ml soluble) + monensin for 5hrs | Monensin | 11B11
| | | mIL-6 | mouse
spleen
| 1) ConA (3µg/ml) for two days 2) IL-2 (20ng/ml) and IL-4 (20ng/ml) for three days
| 2d/3d | anti-CD3 (10µg/ml immobilized) + anti-CD28 (2µg/ml soluble) + monensin for 5hrs | Monensin | MP5-20F3
| | | mIL-10 | mouse
spleen | 1) ConA (3µg/ml) for two days 2) IL-2 (20ng/ml) and IL-4 (20ng/ml) for three days
| 2d/3d | anti-CD3 (10µg/ml immobilized) + anti-CD28 (2µg/ml soluble) + monensin for 5hrs | Monensin | JES5-16E3 | | | | | mGM-CSF | mouse
spleen | 1) ConA (3µg/ml) for two days 2) IL-2 (20ng/ml) and IL-4 (20ng/ml) for three days
| 2d/3d | anti-CD3 (10µg/ml immobilized) + anti-CD28 (2µg/ml soluble) + monensin for 5hrs | Monensin | MP1-22E9 | | | | mIFN-g | mouse
spleen | 1) ConA (3µg/ml) for two days 2) IL-2 (20ng/ml) and IL-4 (20ng/ml) for three days
| 2d/3d | anti-CD3 (10µg/ml immobilized) + anti-CD28 (2µg/ml soluble) + monensin for 5hrs | Monensin | XMG1.2 | | | | mTNF-a | mouse
spleen | 1) ConA (3µg/ml) for two days 2) IL-2 (20ng/ml) and IL-4 (20ng/ml) for three days
| 2d/3d | anti-CD3 (10µg/ml immobilized) + anti-CD28 (2µg/ml soluble) + monensin for 5hrs | Monensin | MP6-XT22 | | | |
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