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TISSUE CULTURE STOCK SOLUTIONS AND MEDIA

TISSUE CULTURE STOCK SOLUTIONS AND MEDIA


MS MEDIUM FOR ARABIDOPSIS

To 990 ml H2O add: Sucrose ........... 10.0  g MOPS ..............  0.5  g Agar ..............  8.0  g  Adjust pH to 5.7 with 1 M KOH. Autoclave.  When cooled add sterile stock solutions of:  KNO3............... 20.0  ml  940 mM (95.00 g/L) NH4NO3............. 20.0  ml 1000 mM (80.04 g/L) KH2PO4............. 12.5  ml  100 mM (13.60 g/L) MgSO47H2O.......... 15.0  ml  100 mM (24.65 g/L) CaCl27H2O.......... 10.0  ml  300 mM (44.00 g/L) Glycine............  2.67 ml   10 mM Nicotinate.........  0.4  ml   10 mM Thiamin............  0.02 ml   10 mM PyridoxineHCl......  0.24 ml   10 mM Myoinositol........  5.5  ml  100 mM FeSO47H2O .........  1.0  ml         (2.780 g/100 ml) Na2EDTA ...........  1.0  ml  100 mM (3.720 g/100 ml) MnSO44H2O..........  1.0  ml  100 mM (1.690 g/100 ml) ZnSO44H2O..........  3.0  ml   10 mM (0.288 g/100 ml) H3BO3 .............  1.0  ml  100 mM (0.618 g/100 ml) KI.................  0.5  ml   10 mM (0.166 g/100 ml) CuSO45H2O .........  0.01 ml   10 mM (0.170 g/100 ml) Na2MoO42H2O .......  0.1  ml   10 mM (0.242 g/100 ml) CoCl26H2O .........  0.01 ml   10 mM (0.238 g/100 ml)  

B5 MEDIUM FOR ARABIDOPSIS

To 990 ml H2O add: Sucrose ........... 20.0 g MOPS .............. 0.5 g Agar .............. 8.0 g Adjust pH to 5.7 with 1 M KOH. Autoclave. When cooled add sterile stock solutions of: KNO3............... 26.3 ml 940 mM (95.00 g/L) NaH2PO4H2O......... 11.0 ml 100 mM MgSO47H2O.......... 10.0 ml 100 mM (24.65 g/L) (NH4)2SO3.......... 10.0 ml 100 mM CaCl27H2O.......... 3.3 ml 300 mM (44.00 g/L) Myoinositol........ 5.5 ml 100 mM Thiamine........... 3.0 ml 10 mM Nicotinate......... 0.8 ml 10 mM PyridoxineHCl...... 0.48 ml 10 mM 10X Micronutrients. 10.0 ml (see below) Sequestrene 330 Fe. 10.0 ml (0.28 g/100 ml) For Callus Inducing Medium (CIM) add: 2,4-D..................... 0.5 ml Kinetin................... 0.05 ml For Shoot Inducing Medium (SIM) add: IAA....................... 0.15 ml 6-dimethylaminopurine..... 5.0 ml
10X Micronutrients (per 100 ml) MnSO44H2O.......... 0.6 ml 100 mM (1.690 g/100 ml) ZnSO44H2O.......... 10.07 ml 10 mM (0.288 g/100 ml) H3BO3 ............. 4.9 ml 100 mM (0.618 g/100 ml) KI................. 4.5 ml 10 mM (0.166 g/100 ml) CuSO45H2O ......... 2.3 ml 10 mM (0.170 g/100 ml) Na2MoO42H2O ....... 1.0 ml 10 mM (0.242 g/100 ml) CoCl26H2O ......... 1.0 ml 10 mM (0.238 g/100 ml)