Archived Protocol

<BODY BACKGROUND="../gif/chalk.gif" TEXT=00009C LINK=8E236B VLINK=CC3299 ALINK=FF1CAE>  <HTML> <HEAD> <DL><DD> <H2>Phosphate (Sodium) buffer Chart</H2> </HEAD> <BODY> Stock solution A 2 M monobasic sodium phosphate, monohydrate (276g/L) Stock solution B 2 M dibasic sodium phosphate (284 g/L). Mixing an appropriate volume (ml) of A and B as shown in the table below and diluting to a total volume of 200 ml, a 1 M phosphate buffer of the required pH at room temperature. <PRE> A B pH 92.0 8.0 0.8 90.0 10.0 5.9 87.7 12.3 6.0 85.5 15.0 6.1 81.5 19.5 6.2 77.5 22.5 6.3 73.5 26.5 6.4 68.5 31.5 6.5 62.5 37.5 6.6 56.5 43.5 6.7 51.0 49.0 6.8 45.0 55.0 6.9 39.0 61.0 7.0 33.0 67.0 7.1 28.0 72.0 7.2 23.0 77.0 7.3 19.0 81.0 7.4 16.0 84.0 7.5 13.0 87.0 7.6 10.5 89.5 7.7 8.5 91.5 7.8 </PRE> </DL> </BODY>  </HTML>