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Phosphate (Sodium) buffer Chart

Stock solution A

2 M monobasic sodium phosphate, monohydrate (276g/L)

Stock solution B

2 M dibasic sodium phosphate (284 g/L).

Mixing an appropriate volume (ml) of A and B as shown in the table below and diluting to a total volume of 200 ml, a 1 M phosphate buffer of the required pH at room temperature.

  A	B	pH   92.0	 8.0	0.8 

90.0 10.0 5.9

87.7 12.3 6.0

85.5 15.0 6.1

81.5 19.5 6.2

77.5 22.5 6.3

73.5 26.5 6.4

68.5 31.5 6.5

62.5 37.5 6.6

56.5 43.5 6.7

51.0 49.0 6.8

45.0 55.0 6.9

39.0 61.0 7.0

33.0 67.0 7.1

28.0 72.0 7.2

23.0 77.0 7.3

19.0 81.0 7.4

16.0 84.0 7.5

13.0 87.0 7.6

10.5 89.5 7.7

8.5 91.5 7.8