This is a cached page for the URL ( To see the most recent version of this page, please click here.
Protocol Online is not affiliated with the authors of this page nor responsible for its content.
About Cache
Protein Extraction from Camiolo et al.
 Reagents: Protein extraction buffer (Camiolo buffer):  100 ml= 	(0.075M Potassium Acetate) 0.736g 		(0.3M) NaCl			1.753g 		(0.1M) L-arginine basic salt	1.742g 		(0.01M) EDTA-HCl		0.292g 		(0.25%) Triton X-100		250. ul 		up to 100 ml with dH20. pH 7.4. Then 0.2 um filter.  1. Freeze tissue in liquid nitrogen. 2. Rinse in PBS then mince. 3. Add 1 ml Camiolo extraction buffer per 100 mg of tissue. 4. Homogenize for 1 minute at 4'C. 5. Spin at 3,000. rpm/15 minutes/4'C. 6. Remove supernatant and save in another tube. 7. If necessary, dialize the supernatant against PBS with  	50mM/L Tris-HCl pH 7.4.