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RESEARCH DIVISION Laboratory Manual

 


 

PMEF Feeder Layer Concentration

 

Area/Well
Flask cm2
Total Area
/cm2
Total Cells 5x104/cm2 Total Vol Conc/ml
96 Well 0.35 37 4.62x105 19.5 2.4x104
24 Well 1.77 42.4 5.3x105 25 2.1x104
12 Well 3.5 41.6 5.2x105 25 1.9x104
6 Well 9.6 57.6 7.0x105 37 1.9x104
Large F1 154 154 1.9x106 20 9.5x104
Med F1  ~175 80 1.0x106 10 1.0x105
Small F1 ~30 24.4 3.0x105 5 6.0x104
Large Dish   143 1.8x106 20 9.0x104
Med Dish   57 0.7x106 10 7.0x104
Small Dish   9.6 1.2x105 2 6.0x104
 
Disclaimer: The following set of protocols were contributed by various members of our lab (past and present): Christine Andrews, Fiona Christensen, Neil Della, Ross Dickins, Debbie Donald, Andrew Holloway, Gary Hime, Colin House, Yinling Hu, Rachael Parkinson, Nadia Traficante, Hannah Robertson, Ping Fu and Dennis Wang. Special thanks to Vicki Hammond, Frank Kontgen and Maria Murphy, who contributed many of the ES cell protocols. Sections dealing with Photomicroscopy, Polyclonal and Monoclonal Antibody Production were provided by members of Gerry Rubin's Laboratory (Berkeley). Any comments in the methods (technical errors etc.) E-mail: d.bowtell@pmci.unimelb.edu.au
David Bowtell PMCI October 1998