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  Answer ID  
80
  Product  
Tech Service: Cell Culture
  Reagents
  Date Created  
08/11/1999 10:58 AM
  Date Updated  
10/10/2002 02:50 PM
  Access Level  
Everyone

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  Dose of G418 to be used for selection
  Question
  What dose of Geneticin (G418) should I use for selection?
  Answer
  Sensitivity to G418 differs markedly depending on cell type, cell growth medium, and the serum supplement used. Experimentally determine the proper concentration for selection by setting up a dose response curve. Use concentrations between 100 ug/ml and 2 mg/ml G418 in medium, at 200 ug/ml increments. For more information, see product insert.
 
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