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Tech Service: Cell Culture
  Date Created  
08/11/1999 10:58 AM
  Date Updated  
10/10/2002 02:50 PM
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  Dose of G418 to be used for selection
  What dose of Geneticin (G418) should I use for selection?
  Sensitivity to G418 differs markedly depending on cell type, cell growth medium, and the serum supplement used. Experimentally determine the proper concentration for selection by setting up a dose response curve. Use concentrations between 100 ug/ml and 2 mg/ml G418 in medium, at 200 ug/ml increments. For more information, see product insert.
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