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Recommended Protocols


Day- by-day Protocol Menu

Click on the calendar day to review these daily protocol instructions, or alternatively go to the download center to retrieve a full copy as a file.

  • Thawing MEF cells, expansion and inactivation
  • Thawing and feeding ES cells
  • Electroporation
  • Feeding transfected ES cells in Selection Media
  • Picking ES clones
  • Disaggregation, expansion and freezing of transfected ES clones
  • Expansion of your targeted ES clones
  • Transient transfection protocol for pTurbo-cre
  • ..Genomic DNA preparation from ES cells
  • Mouse Tail (or organ) Biopsy DNA Extraction
  • Preparation of Murine Embryonic Fibroblast (MEF) feeder cells
  • Recommended reagent sources and recipes