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Screening of lambda libraries
Screening of cDNA and genomic libraries. 

Choice of bacterial strain:

The bacterial E. coli strains we use when plating out cDNA and genomic libraries are normally LE392 and NM538, but some times we also use XL1-blue cells.
 
 

Checking the titer of the library:
 

 

Plating out the library:
 
 

Taking lifts of the plates, (transferring the lambda phage to nylon membranes).
 
 

 
 

Probe labeling:
 
 

Filter Hybridization:
 
 

 

Washing of the filters:
 
 



 


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