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Screening of lambda libraries
Screening of cDNA and genomic libraries. 

Choice of bacterial strain:

The bacterial E. coli strains we use when plating out cDNA and genomic libraries are normally LE392 and NM538, but some times we also use XL1-blue cells.

Checking the titer of the library:


Plating out the library:

Taking lifts of the plates, (transferring the lambda phage to nylon membranes).


Probe labeling:

Filter Hybridization:


Washing of the filters:


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