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Screening a cDNA Library

Screening a cDNA Library

for use with HybriZAP zebrafish cDNA libraries

Objective

cDNA library screening allows detection of expressed genes for subsequent cloning and sequencing.

Selecting a Library

Currently, our lab has access to cDNA libraries from three developmental time points. Choose the appropriate library for when you expect your message of interest is expressed.

Overview of Procedures

  1. Titer the library to determine the concentration of infectious phage.
  2. Plate and lift the phage particles.
  3. Hybridize the immobilized cDNA with labeled probe.
  4. Pick positive plaques.
  5. Cycle through steps 2-4 until a single cDNA containing phage is isolated, generally three cycles.

Direct questions or comments concerning this web page to Karl Clark: kclark@biosci.cbs.umn.edu
Last modified September 04, 1997
URL = http://biosci.cbs.umn.edu/~kclark/protocols/library.html%3CBR>
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