June 7, 1990
Special reagents required:
Special equipment required:
Use a sterile beaker and keep covered.
OD260 of undiluted solution = 0.28 x 300 = 84.0
Since an OD260 reading of 1.0 = 50 µg/ml of DNA, the concentration of the undiluted solution is 50 x 84 = 4200 µg/ml, or 4.2 mg/ml.
If the present volume is 100 ml (420 mg), add enough TE to adjust the final concentration to 2.5 mg/ml. (In this case, add 68 ml TE to bring the final volume to 168 ml.)