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DNA Preparation | |
Preparation of DNA Samples (updated 12/3/99) | |
Slide Preparation | |
Poly-l-Lysine coating of slides (updated 10/6/99) | |
Directions for Printing Arrays | |
RNA amplification | |
aRNA Amplification (updated 6/15/01) | |
Nucleic Acid Preparation | |
Human FastTrack mRNA Isolation (updated 8/9/99) Preparation of total RNA from whole blood collected in PAXgene tubes (updated 6/18/02) | |
Nucleic Acid Labeling | |
Reverse Transcription using Cy3- & Cy5-dUTP: ---Yeast (updated 3/29/99) ---Human (updated 3/15/01) ---E. coli (updated 2/22/99) Reverse Transcription and aa-UTP Labeling of RNA (updated 6/28/00) Revised amino-allyl labeling protocol (updated 2/3/01) Labeling Genomic DNA (updated 11/17/00) | |
Array Post Processing | |
Post-Processing of Arrays (updated 9/19/99) | |
Array Hybridization | |
Array Hybridization-General/Yeast (updated 8/10/99) Array Hybridization-Human (updated 3/15/01) | |
Scanning Microarrays | |
Data Processing | |
Miscellaneous | |
Cy dye HPLC purification Design of Yeast Genomic Expression Experiments |
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