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Protocol Matches: 13
- Western Blot (R&D System)
Provides very nice and general protocol including Western Blot Procedure, immunostaining Procedure (standard and rapid), helpful hints and trouble shooting guide
http://www.rndsystems.com/western_blot_detail_obje...
Added: Tue May 14 2002, Hits: 11237, Reviews: 0 Write review Cached - Western blot troubleshooting - Blank Film
(J. Rusty Bishop, PhD)
3 hr and 33 minute protocol to troubleshooting the major causes of blank film in a Western blot.
http://westernblot.org/western-blot-troubleshootin...
Added: Wed Aug 17 2011, Hits: 1100, Reviews: 0 Write review - Western Blot and Membrane Stripping
(Cowin Lab, New York University Medical Center)
Describes procedures for Western blotting and stripping blot for reblotting.
http://celljunctions.med.nyu.edu/lab/notprivate/me...
Added: Mon Mar 16 2009, Hits: 11863, Reviews: 0 Write review -
Too Many Bands on Western Blot (Synaptic Systems)
It is not uncommon that, contrary to the theoretical predictions, several bands are detected. Although it is possible that the antibody is not entirely specific for the protein, other factors may be responsible
http://www.sysy.com/faqs/western.html
Added: Tue May 14 2002, Hits: 1525, Reviews: 0 Write review Cached - Western Blot with Platelet Protein
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
Detailed western protocol devoted to detecting paltelet protein but can also be used for general purpose.
http://www.icp.ucl.ac.be/mexp/vipg_plwb.html
Added: Mon Mar 22 2004, Hits: 2190, Reviews: 0 Write review Cached -
Preparation of Brain Membrane Fractions for Western Blot Analysis
(BD PharMingen)
This is a standard protocol adapted from Rogers et al., 1991.
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Tue May 14 2002, Hits: 2578, Reviews: 0 Write review Cached -
Preparation of Brain Membrane Fractions for Western Blot Analysis
(BD PharMingen)
This is a standard protocol adapted from Rogers et al., 1991.
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Tue May 14 2002, Hits: 2852, Reviews: 0 Write review Cached -
Semi-Dry Western Blot Transfer
(Morimoto Lab, Northwestern University)
In semi-dry blotting the electrodes are placed directly in contact with the gel/nitrocellulose membrane sandwich to provide a fast, efficient transfer.
http://www.biochem.northwestern.edu/morimoto/resea...
Added: Mon Mar 16 2009, Hits: 7527, Reviews: 0 Write review - Western Blot (Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
Describes sample preparation, SDS-PAGE, staining, transfer, blotting
http://www.its.caltech.edu/%7Ebjorker/Western_blot...
Added: Wed Aug 14 2002, Hits: 23241, Reviews: 0 Write review -
Yeast Protein Preparation for SDS PAGE and Western (Haha Lab)
Yeast whole cell protein extraction for western blot using glass bead method
http://labs.fhcrc.org/hahn/Methods/biochem_meth/ye...
Added: Tue May 14 2002, Hits: 2707, Reviews: 0 Write review Cached - Stripping Western Membranes (Contributed by Monica Colitti)
Simple protocol for reusing blot
Added: Tue May 14 2002, Reviews: 0 Write review - Plant Molecular Biology Protocols (By John Mundy, hosted by The Arabidopsis Information Resource)
A large collection of protocols on plant work especially on arabidopsis. All on one page including these topics: Agrobacterium competent cells / Agrobacterium electroporation / Agrobacterium miniprep / Agrobacterium miniprep (phenol free) / Arabidopsis transformation (infiltration) / cDNA probes / Colony hybridization / Concanavalin A Blots / DNA PAGE / E coli competent cells (CaCl2) / E coli competent cells (RuCl2) / E coli transformation / End labelled probe preparation / Filter washing / Fluorography of PAG / Fluorometer/Luminometer Wallac Victor II / Footprint with DNAse1 / Gelshift / Genomic DNA from cereal leaves / GST fusion protein purification / GUS histochemical assay / GUS & LUC bombardment assay / GUS luminometric assay / HIS-tagged protein purification / Hormone treatments / Hormones & inhibitors / Hybridization selection of cloned DNA / Immunoprecipitation from in vitro translations / Isotachophoresis probe isolation / In vitro transcription / In vitro translation / Isolation of Lambda DNA by plate lysate / Lambda lysate / Leaf PCR / Maxam Gilbert sequencing / Microsomal preps for membrane proteins / Miniprep - alkaline lysis / NEpHGE Non-equilibrium pH gel electrophoresis / Northern blot in formaldehyde gel / Nuclear extract preparation / Nuclei prep / Nuclear prep from protoplasts / Oligo dT cellulose purification of mRNA / Oligo probe hybridization / Oligonucleotide probe preparation / Phage strains / Plasmid prep with CsCl / Primer extension with synthetic oligonucleotide / Progeny analysis of plants / RNA extraction in GT w/ CsCl spin / RNA large scale from starchy tissue / RNA miniprep 1 / RNA miniprep 2 / RT-PCR / SDS-PAGE for proteins / Seed sterilization for cereals / Silver staining of proteins in PAG / Soluble protein extraction / Southwestern blotting / T4 polymerase fill in rxn / Western blotting / Whole cell extracts /
http://www.arabidopsis.org/info/Protocols_Mundy2.h...
Added: Tue Jul 30 2002, Hits: 2186, Reviews: 0 Write review Cached - Cell-Free System for the Examination of Apoptotic Activity (CellDeath.de)
Activation of apoptotic activity in cytoplasmic extracts and measurement of the resulting caspase enzymatic acticity,br>Activation of apoptotic activity in cytoplasmic extracts western blot analysis Reconstitution of activated cytoplasmic extracts with isolated nuclei. Analysis of apoptotic activity by qualitative DNA laddering assay and DAPI staining Reconstitution of activated cytoplasmic extracts with isolated radioactive nuclei. Analysis of apoptotic activity by quantitative, radioactive DNA fragmentation assays or DAPI staining.
http://www.celldeath.de/apometh/cfsystem.html
Added: Sat Jan 31 2009, Hits: 2560, Reviews: 0 Write review Cached