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Protocol Matches: 49

• Standard Staining Procedures (The Internet Pathology Laboratory for Medical Education, Florida State University College of Medicine)
  Standardized staining procedures for histology laboratory. The following are described:
  • Acid Fast Stain (for mycobacteria)
  • Acid Fast Stain 
  • Alcian Blue Stain
  • Alcian Blue-PAS Stain (PAB)
  • Hyaluronidase Digestion for Alcian Blue
  • Alizarin Stain for calcium
  • Auramine-Rhodamine Stain (fluorescent)
  • Bielschowsky Stain (for senile plaques)
  • Bile Stain
  • Bodian's Stain
  • Colloidal Iron Stain
  • Congo Red Stain
  • Copper Stain
  • Elastic van Gieson Stain
  • Elastic - Weigert's resorcin-fuchsin method
  • Modified Elastic van Gieson Stain
  • Fontana-Masson Stain for melanin
  • Melanin Bleach
  • Fraser Lendrum Stain
  • Giemsa (Modified May-Gruenwald) Stain (for hematopoietic tissues)
  • Giemsa Stain (for Helicobacter)
  • Gram (Modified Brown-Brenn) Stain
  • Gridley's Stain for ameba
  • Grimelius Argyrophil Stain (Pascual's Method)
  • Grocott's Methenamine Silver (GMS) Stain
  • Holzer's Glial Fiber Stain
  • Hortega's Pineal Stain
  • Iron Stain (Prussian blue)
  • Iron Stain (Turnbull's blue)
  • Jones' Silver Stain
  • Luxol Fast Blue (LFB) Stain
  • Methyl Green Pyronin (MGP) Stain
  • Mucicarmine Stain
  • Nissl Stain
  • Oil Red O Stain
  • Orcein Stain
  • Periodic acid-Schiff Stain (PAS)
  • Periodic acid-Schiff, digested Stain (PAS-D)
  • PTAH Stain
  • Reticulin Stain
  • Spirochete Stain (Steiner & Steiner method)
  • Sudan Black B Stain (for lipochrome)
  • Sudan Black B Stain (for fat)
  • Trichrome Stain - Masson's method
  • Trichrome Stain - microwave method
  • Thioflavin S Stain (for amyloid in tissues)
  • Modified Thioflavin S Stain (for senile plaques)
  • Toluidine Blue Stain (for mast cells)
  • Urate Crystal Stain
  • VonKossa Stain for calcium
  
  http://medlib.med.utah.edu/WebPath/HISTHTML/MANUAL...
  Added: Sat Oct 05 2002, Hits: 26359, Reviews: 0  Write review   Cached
• Guide to Special Stains (Dako)
  This handbook guides you through fixation, tissue processing and the different staining methods. Content includes: Introduction to Special Stains Historical Perspective 1; Clinical Relevance 2 The Biology of Special Stains The Cell 5; Tissue 6; Pathogens 8 The Chemistry of Special Stains Basic Chemistry 14; Principles Of Staining 15 Fixation and Tissue Processing Fixation 17; Specimen Processing 21 Staining Methods: Nucleus and Cytoplasm Hematoxylin and Eosin 24; Nucleic Acids 29; Polychromatic Stains 31 Staining Methods:Connective Tissue, Muscle Fibers and Lipids Connective Tissue 34; Reticular Fibers 35; Basement Membranes 36; Elastic Fibers 38; Mast Cells 39; Muscle 41; Trichrome Stains 41; PTAH 44; Modified Russel-Movat Pentachrome 45; Lipids 46 Staining Methods: Carbohydrates and Amyloid Carbohydrates 48; Amyloid 48; Periodic Acid-Schiff (PAS) 49; PAS with Diastase 51; Mucicarmine 52; Alcian Blue 53; Alcian Blue/PAS 55; Colloidal Iron 56; Congo Red 57 Staining Methods: Microorganisms Bacteria 60; Fungi 61; Protozoans 61; Gram Stain 62; Acid-Fast Bacteria Stain 63; Grocott’s Methenamine Silver Stain 64; Warthin-Starry Stain 65; Alcian Yellow/Toluidine Blue Stain 66; Giemsa Stain 68; Periodic Acid-Schiff-Green Stain 69 Staining Methods: Pigments and Minerals Melanin 71; Hemosiderin 73; Lipofuscin 74; Urates 75; Bile 76; Calcium 77; Copper 78 Staining Methods: Nervous System Cresyl Violet Stain 80; Bielschowsky Method 81; Glial Cells 82; Myelin 84 Automation of Special Stains Overview 86; Advantages 86; Considerations
  http://pri.dako.com/08066_guide_to_special_stains....
  Added: Fri Apr 03 2009, Hits: 8986, Reviews: 0  Write review  
• Conventional Giemsa Stain 2 (Primate Cytogenetics Network)
  Conventional staining techniques are used to uniformly stain chromosomes and leave the centromeres constricted, thus enabling the measurement of chromosome length, centromeric position, and arm ratio.
  http://homepage.mac.com/wildlifeweb/cyto/text/Giem...
  Added: Fri Apr 02 2010, Hits: 703, Reviews: 0  Write review   Cached
• Protein Silver Stain Protocol (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
  Silver Stain is about 10 to 100 times more sensitive than Coomasie Blue Staining; at least 1ng protein using this protocol.
  http://wolfson.huji.ac.il/purification/Protocols/S...
  Added: Sat Feb 21 2009, Hits: 2363, Reviews: 0  Write review   Cached
• Conventional Giemsa Stain (Primate Cytogenetics Network)
  Conventional staining techniques are used to uniformly stain chromosomes and leave the centromeres constricted, thus enabling the measurement of chromosome length, centromeric position, and arm ratio.
  http://homepage.mac.com/wildlifeweb/cyto/text/Giem...
  Added: Fri Apr 02 2010, Hits: 1161, Reviews: 0  Write review   Cached
• Silver Stain for SDS PAGE (The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
  http://labs.fhcrc.org/hahn/Methods/biochem_meth/si...
  Added: Tue May 14 2002, Hits: 1977, Reviews: 0  Write review   Cached
• Tissue Stain for Nissl Substance-Cresyl Fast Violet Technique (Nottingham Pathology Lab)
  http://www.nottingham.ac.uk/pathology/protocols/cf...
  Added: Tue May 14 2002, Hits: 979, Reviews: 0  Write review   Cached
• Tissue Stain for Amyloid- ( )
  Alkaline Congo Red Technique (Nottingham Pathology Lab)
  http://www.nottingham.ac.uk/pathology/protocols/al...
  Added: Tue May 14 2002, Hits: 1257, Reviews: 0  Write review   Cached
• Acromosal stain (P. J. Hansen Lab)
  http://www.animal.ufl.edu/hansen/protocols/acrstai...
  Added: Tue May 14 2002, Hits: 477, Reviews: 0  Write review   Cached
• Tissue Stain for Mucins-Alcian Blue technique (Nottingham Pathology Lab)
  http://www.nottingham.ac.uk/pathology/protocols/al...
  Added: Tue May 14 2002, Hits: 1035, Reviews: 0  Write review   Cached
• JC-1 Stain of Apoptotic Cells (Research Group of Johannes Schmid, Medical University of Vienna)
  JC1-staining to detect apoptotic cells via the mitochondrial membrane potential.
  http://www.meduniwien.ac.at/user/johannes.schmid/J...
  Added: Fri Nov 06 2009, Hits: 1105, Reviews: 0  Write review   Cached
• Leishman's Stain (Primate Cytogenetics Network)
  http://homepage.mac.com/wildlifeweb/cyto/text/Leis...
  Added: Fri Apr 02 2010, Hits: 292, Reviews: 0  Write review   Cached
• Human Alkaline Phosphatase Stain (Miller lab, Fred Hutchinson Cancer Research Center)
  This protocol is for detection of human placental alkaline phosphatase (AP) in cultured cells.
  http://labs.fhcrc.org/miller/protocols/hpapstain.h...
  Added: Wed Feb 04 2009, Hits: 2865, Reviews: 0  Write review   Cached
• Tissue Stain for Axons and Alzheimer's Disease- ( )
  Bielschowsky Method (Nottingham Pathology Lab)
  http://www.nottingham.ac.uk/pathology/protocols/bi...
  Added: Tue May 14 2002, Hits: 862, Reviews: 0  Write review   Cached
• Protein Gel Staining Using Coomassie Blue Protein Stain (OpenWetWare)
  http://openwetware.org/wiki/Jacobs:Protocol_Protei...
  Added: Fri Mar 20 2009, Hits: 2597, Reviews: 0  Write review  
• Double Immunofluorescence stain (Giorgio Cattoretti, Columbia University)
  Any type of tissue is suitable for this technique, as long as the antigenicity for your antigen(s) is preserved. Use cytocentrifuged cells or frozen or paraffin dewaxed sections
  http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
  Added: Fri Aug 30 2002, Hits: 4398, Reviews: 0  Write review   Cached
• Negative Stain Electron Microscopy of Microtubules (Mitchison Lab)
  http://mitchison.med.harvard.edu/protocols/EM.html
  Added: Tue May 14 2002, Hits: 183, Reviews: 0  Write review   Cached
• Dye Filling to Stain Amphid and Phasmid Neurons (Michael Koelle) (Ambros Lab)
  http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambro...
  Added: Tue May 14 2002, Hits: 399, Reviews: 0  Write review   Cached
• Silver Stain for SDS PAGE (The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
  http://labs.fhcrc.org/hahn/Methods/biochem_meth/si...
  Added: Tue May 14 2002, Hits: 698, Reviews: 0  Write review   Cached
• Dye Filling to Stain Amphid and Phasmid Neurons: ( )
  Michael Koelle
  http://www.dartmouth.edu/artsci/bio/ambros/protoco...
  Added: Tue May 14 2002, Hits: 57, Reviews: 0  Write review   Cached
• Replication Banding (Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
  Provides differential staining of early and late replicating areas of the chromosomes. Allows identification of the early replicating X chromosome. Can be used for marker chromosome analysis. When Bromo deoxyuridine is added at the end of culture late replicating areas will stain pale and early replicating areas will stain dark.
  http://www.slh.wisc.edu/cytogenetics/Protocols/Sta...
  Added: Mon Dec 09 2002, Hits: 59, Reviews: 0  Write review   Cached
• Trypan Blue Viability Test (Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
  Trypan blue will stain dead or dying cells. Viable cells are able to repell the dye and do not stain. Note: Trypan blue has a greater affinity for serum proteins than for cellular protein. If the background is too dark, cells should be pelleted and resuspended in protein-free medium or salt solution prior to counting.
  http://www-ufk.med.uni-rostock.de/lablinks/protoco...
  Added: Mon Oct 28 2002, Hits: 6210, Reviews: 0  Write review   Cached
• Receipes (Universitäts-Frauenklinik Rostock)
  Receipes for commonly used reagents, culture media, solutions including: Acetone / Formaldehyde Fixative, Acrylamide Gel, denaturing, Acrylamide Gel, nondenaturing, Alkaline Phosphatase Buffer 1 (Glycine Buffer, 0.1 M, pH 10.4), Alkaline Phosphatase Buffer 2 (Tris-HCl, 100 mM, pH 9.5), Alkaline Phospatase Stain (NBT + BCIP), Amido Black Destain, Ammonium Bicarbonate Buffer, 100x, Annealing Buffer, Antibiotics for Microbiology, Aprotinin Stock Solution, BLOTTO 1 x, Bouins Fixative, Butanol, water saturated, Cadmium Chloride Stock Solution, Calcium Chloride for Competent Cells, Calcium Chloride Stock Solution, CIP Buffer (10x), Coomassie Destain, Coomassie Stain, Crystal Violet Stock Solution, Cytosol Preparation Buffer, DEAE DNA Elution Buffer I, DEAE DNA Elution Buffer II, DEAE Membrane Wash Buffer, Denhardt's, 50 x, DMEM/MCDB-302, DMEM, DTT Stock Solution, EDTA Stock Solution, 0.5 M, EMSA Binding Buffer, Fischer's Medium (for Nb2 cells), Glycerol Stock Solution, 80%, HBS (HEPES buffered saline), HBSS (Hanks Balanced Salt Solution), HBHA (Hanks Balanced Salt Solution with BSA, HEPES, NaN3), HeBS (Hepes buffered saline) buffer for electroporation, HEPES Stock Solution, IL-2 Stock Solution, Immunoprecipitation buffer (cell lysis buffer 2), Immunoprecipitation dilution buffer, Immunoprecipitation wash buffer, IPTG Stock, KCl Stock Solution, 2 M, LB Agar, LB Medium, Lower Tris Buffer, 4x, Magnesium Chloride Stock Solution, MEM, NaCl Stock, 5 M, NaOH Stock, 10 N, NCTC (medium for RCHO-1 cells), NZY broth, NZY plates, PBS / EDTA solution, PBS Stock Solution, PIPES Stock Solution, PMSF Stock 0.1 M, R10 (medium for KY1 and KY2 cells), RIPA buffer (cell lysis buffer 1), RNK (medium for RNK-16 cells), RPMI 1640 (medium for YAC-1 cells), SDS-PAGE, 4 x Running Buffer, SDS-PAGE, 4 x Sample Buffer, SDS Stock Solution, 10%, 20%, SM buffer, SOB-medium, SOC-medium, Sodium Acetate, 3 M, SSC, 20 x, SSPE, 20 x, Stacking Gel, STE, 1 X (for Nuc Trap), STE for Genomic DNA Isolation, TAE Buffer Stock Solution, 50 x, TBE Stock Solution, 5 x, TBS/TBST buffer, 10x, TDMN Buffer, TE Buffer, Top Agar, Transfer Buffer (for Western Blotting), Tris Stock Solution, 1 M, Upper Tris Buffer, 4x, Zinc Chloride Stock Solution,
  http://www-ufk.med.uni-rostock.de/lablinks/recipes...
  Added: Mon Oct 14 2002, Hits: 4324, Reviews: 0  Write review   Cached
• Blood Smear Preparation and Staining (The University of Texas Medical School,Department of Pathology and Laboratory Medicine)
  A thin blood smear is stained with Wright-Giemsa stain to enable evaluation of white blood cell, red cell and platelet morphology.
  http://medic.med.uth.tmc.edu/path/00000288.htm
  Added: Sun Mar 21 2004, Hits: 248, Reviews: 0  Write review   Cached
• C-Banding (Primate Cytogenetics Network)
  To specifically stain the centromeric regions and other regions containing constitutive heterochromatin, i.e., the secondary contrictions of human chromsomes 1, 9, 16, and the distal segment of the Y chromosome long arm.
  http://homepage.mac.com/wildlifeweb/cyto/text/C-ba...
  Added: Fri Apr 02 2010, Hits: 320, Reviews: 0  Write review   Cached

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