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Protocol Matches: 10
- Protein Extraction from Tissues
(Reddy research laboratory, Neurological Sciences Institute, Oregon Health & Science University)
protein extraction from tissues, lymphoblasts or fibroblasts.
http://www.ohsu.edu/nsi/faculty/reddyh/lab/protpro...
Added: Mon Sep 23 2002, Hits: 13601, Reviews: 0
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Cached - Protein Extraction from Plant
(The University of Nebraska-Lincoln Protein Core Facility )
A common protocol used for extraction proteins from plant tissues, consists in the homogenization of mortar-grounded material in liquid nitrogen with an extraction buffer (20 mM Tris-HCl, pH 8.0, 5 mM EDTA, 50 M leupeptin, 1 M pepstatin A, 10 M 3, 4-dichloroisocumarine, 1 mM phenylmethylsulfonyl fluoride and 0.05 % SDS)...
http://www.biotech.unl.edu/proteomics/Mercaptoetha...
Added: Tue Aug 13 2002, Hits: 5066, Reviews: 1 Read reviews
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Cached - Bacterial Protein Extraction at mini-scale using B-Per (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
A useful procedure for initial screening of expression conditions instead of sonication of bacteria; not recommended for large scale preparation of protein.
http://wolfson.huji.ac.il/purification/TagProteinP...
Added: Sat Feb 21 2009, Hits: 7240, Reviews: 0
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Cached - Protein Extraction (Frank Bottone Jr.)
http://www.unc.edu/~fbottone/protocol/protein.html
Added: Tue May 14 2002, Hits: 15523, Reviews: 0
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Cached -
RNA and protein extraction from the same tissue
(Ivan J Delgado)
http://ivaan.com/protocols/139.html
Added: Sun Mar 01 2009, Hits: 6233, Reviews: 0
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Cached - Bacterial Protein Extraction (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
Protocol for mini prep using sonication
http://wolfson.huji.ac.il/purification/TagProteinP...
Added: Sat Feb 21 2009, Hits: 18993, Reviews: 0
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Cached -
Isolation of Protein from Tissue
(Reddy research laboratory, Neurological Sciences Institute, Oregon Health & Science University)
Nice formated protocol on protein extraction from tissues, lymphoblasts or fibroblasts.
http://www.ohsu.edu/nsi/faculty/reddyh/lab/protpro...
Added: Thu Oct 03 2002, Hits: 10871, Reviews: 0
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Cached - Plant Molecular Biology Protocols (By John Mundy, hosted by The Arabidopsis Information Resource)
A large collection of protocols on plant work especially on arabidopsis. All on one page including these topics: Agrobacterium competent cells / Agrobacterium electroporation /
Agrobacterium miniprep /
Agrobacterium miniprep (phenol free) /
Arabidopsis transformation (infiltration) /
cDNA probes /
Colony hybridization /
Concanavalin A Blots /
DNA PAGE /
E coli competent cells (CaCl2) /
E coli competent cells (RuCl2) /
E coli transformation /
End labelled probe preparation /
Filter washing /
Fluorography of PAG /
Fluorometer/Luminometer Wallac Victor II /
Footprint with DNAse1 /
Gelshift /
Genomic DNA from cereal leaves /
GST fusion protein purification /
GUS histochemical assay /
GUS & LUC bombardment assay /
GUS luminometric assay /
HIS-tagged protein purification /
Hormone treatments /
Hormones & inhibitors /
Hybridization selection of cloned DNA /
Immunoprecipitation from in vitro translations /
Isotachophoresis probe isolation /
In vitro transcription /
In vitro translation /
Isolation of Lambda DNA by plate lysate /
Lambda lysate /
Leaf PCR /
Maxam Gilbert sequencing /
Microsomal preps for membrane proteins /
Miniprep - alkaline lysis /
NEpHGE Non-equilibrium pH gel electrophoresis /
Northern blot in formaldehyde gel /
Nuclear extract preparation /
Nuclei prep /
Nuclear prep from protoplasts /
Oligo dT cellulose purification of mRNA /
Oligo probe hybridization /
Oligonucleotide probe preparation /
Phage strains /
Plasmid prep with CsCl /
Primer extension with synthetic oligonucleotide /
Progeny analysis of plants /
RNA extraction in GT w/ CsCl spin /
RNA large scale from starchy tissue /
RNA miniprep 1 /
RNA miniprep 2 /
RT-PCR /
SDS-PAGE for proteins /
Seed sterilization for cereals /
Silver staining of proteins in PAG /
Soluble protein extraction /
Southwestern blotting /
T4 polymerase fill in rxn /
Western blotting /
Whole cell extracts /
http://www.arabidopsis.org/info/Protocols_Mundy2.h...
Added: Tue Jul 30 2002, Hits: 2262, Reviews: 0
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Cached -
Yeast Protein Preparation for SDS PAGE and Western
(Haha Lab)
Yeast whole cell protein extraction for western blot using glass bead method
http://labs.fhcrc.org/hahn/Methods/biochem_meth/ye...
Added: Tue May 14 2002, Hits: 2819, Reviews: 0
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Cached -
Non-Radioactive EMSA Protocol
(Hammer Lab, University of Michigan)
Procedures include probe preparation by PCR amplifying a fragment of the promoter of interest using DIG-labeled dUTP in addition to normal dNTPs, Standard Nuclear Protein Extraction, Protein/Probe Binding, Electrophoresis, transfer and DIG detection
http://www.med.umich.edu/hammerlab/Protocols/Proto...
Added: Mon Mar 16 2009, Hits: 5302, Reviews: 0
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