 |
Search Protocol Section using Google:
Search Results
Category Matches: 1
Protocol Matches: 10
- Protein Extraction from Tissues
(Reddy research laboratory, Neurological Sciences Institute, Oregon Health & Science University)
protein extraction from tissues, lymphoblasts or fibroblasts.
http://www.ohsu.edu/nsi/faculty/reddyh/lab/protpro...
Added: Mon Sep 23 2002, Hits: 2972, Reviews: 0 Write review Cached - Protein Extraction from Plant
(The University of Nebraska-Lincoln Protein Core Facility )
A common protocol used for extraction proteins from plant tissues, consists in the homogenization of mortar-grounded material in liquid nitrogen with an extraction buffer (20 mM Tris-HCl, pH 8.0, 5 mM EDTA, 50 M leupeptin, 1 M pepstatin A, 10 M 3, 4-dichloroisocumarine, 1 mM phenylmethylsulfonyl fluoride and 0.05 % SDS)...
http://www.biotech.unl.edu/proteomics/Mercaptoetha...
Added: Tue Aug 13 2002, Hits: 1126, Reviews: 1 Read reviews Write review Cached - Bacterial Protein Extraction at mini-scale using B-Per (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
A useful procedure for initial screening of expression conditions instead of sonication of bacteria; not recommended for large scale preparation of protein.
http://wolfson.huji.ac.il/purification/TagProteinP...
Added: Sat Feb 21 2009, Hits: 913, Reviews: 0 Write review Cached - Protein Extraction (Frank Bottone Jr.)
http://www.unc.edu/~fbottone/protocol/protein.html
Added: Tue May 14 2002, Hits: 4075, Reviews: 0 Write review Cached -
RNA and protein extraction from the same tissue
(Ivan J Delgado)
http://ivaan.com/protocols/139.html
Added: Sun Mar 01 2009, Hits: 1015, Reviews: 0 Write review Cached - Bacterial Protein Extraction (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
Protocol for mini prep using sonication
http://wolfson.huji.ac.il/purification/TagProteinP...
Added: Sat Feb 21 2009, Hits: 2247, Reviews: 0 Write review Cached -
Isolation of Protein from Tissue
(Reddy research laboratory, Neurological Sciences Institute, Oregon Health & Science University)
Nice formated protocol on protein extraction from tissues, lymphoblasts or fibroblasts.
http://www.ohsu.edu/nsi/faculty/reddyh/lab/protpro...
Added: Thu Oct 03 2002, Hits: 2683, Reviews: 0 Write review Cached - Plant Molecular Biology Protocols (By John Mundy, hosted by The Arabidopsis Information Resource)
A large collection of protocols on plant work especially on arabidopsis. All on one page including these topics: Agrobacterium competent cells / Agrobacterium electroporation / Agrobacterium miniprep / Agrobacterium miniprep (phenol free) / Arabidopsis transformation (infiltration) / cDNA probes / Colony hybridization / Concanavalin A Blots / DNA PAGE / E coli competent cells (CaCl2) / E coli competent cells (RuCl2) / E coli transformation / End labelled probe preparation / Filter washing / Fluorography of PAG / Fluorometer/Luminometer Wallac Victor II / Footprint with DNAse1 / Gelshift / Genomic DNA from cereal leaves / GST fusion protein purification / GUS histochemical assay / GUS & LUC bombardment assay / GUS luminometric assay / HIS-tagged protein purification / Hormone treatments / Hormones & inhibitors / Hybridization selection of cloned DNA / Immunoprecipitation from in vitro translations / Isotachophoresis probe isolation / In vitro transcription / In vitro translation / Isolation of Lambda DNA by plate lysate / Lambda lysate / Leaf PCR / Maxam Gilbert sequencing / Microsomal preps for membrane proteins / Miniprep - alkaline lysis / NEpHGE Non-equilibrium pH gel electrophoresis / Northern blot in formaldehyde gel / Nuclear extract preparation / Nuclei prep / Nuclear prep from protoplasts / Oligo dT cellulose purification of mRNA / Oligo probe hybridization / Oligonucleotide probe preparation / Phage strains / Plasmid prep with CsCl / Primer extension with synthetic oligonucleotide / Progeny analysis of plants / RNA extraction in GT w/ CsCl spin / RNA large scale from starchy tissue / RNA miniprep 1 / RNA miniprep 2 / RT-PCR / SDS-PAGE for proteins / Seed sterilization for cereals / Silver staining of proteins in PAG / Soluble protein extraction / Southwestern blotting / T4 polymerase fill in rxn / Western blotting / Whole cell extracts /
http://www.arabidopsis.org/info/Protocols_Mundy2.h...
Added: Tue Jul 30 2002, Hits: 2094, Reviews: 0 Write review Cached -
Yeast Protein Preparation for SDS PAGE and Western
(Haha Lab)
Yeast whole cell protein extraction for western blot using glass bead method
http://labs.fhcrc.org/hahn/Methods/biochem_meth/ye...
Added: Tue May 14 2002, Hits: 1343, Reviews: 0 Write review Cached -
Non-Radioactive EMSA Protocol
(Hammer Lab, University of Michigan)
Procedures include probe preparation by PCR amplifying a fragment of the promoter of interest using DIG-labeled dUTP in addition to normal dNTPs, Standard Nuclear Protein Extraction, Protein/Probe Binding, Electrophoresis, transfer and DIG detection
http://www.med.umich.edu/hammerlab/Protocols/Proto...
Added: Mon Mar 16 2009, Hits: 933, Reviews: 0 Write review