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  • Cross Linking and Immunoprecipitation (CLIP) PDF file (Laboratory of Molecular Neuro-Oncology, Rochefeller Univ.)
    CLIP or cross-linking and immunoprecipitation assay exploits the covalent protein-nucleic acid cross-linking to stringently purify a specific protein-RNA complex using immunoprecipitation followed by SDS-PAGE separation. In this way, the vast majority of non-specific contaminating RNA, which can bind to co-immunoprecipitated proteins or beads, can be removed. Here is an improved protocol that performs RNA linker ligation before the SDS-PAGE step, and describe its application to the specific purification and amplification of RNA ligands of Nova in neurons.
    http://www.rockefeller.edu/labheads/darnellr/CLIP_...
    Added: Sat Jan 31 2009, Hits: 4969, Reviews: 0  Write review  
  • Immunoprecipitation Protocol (eBioscience)
    Immunoprecipitation involves the interaction between a protein and its specific antibody, the separation of these immune complexes with Protein G or Protein A, and the subsequent analysis by SDS-PAGE. This technique provides a rapid and simple means to separate a specific protein from whole cell lysates or culture supernatants. This protocol offers a general guideline for immunoprecipitation.
    http://www.ebioscience.com/ebioscience/appls/IP.ht...
    Added: Sat Jan 31 2009, Hits: 8813, Reviews: 0  Write review   Cached
  • Immunoprecipitation (IP) Protocols (Sigma)
    Describes two methods of immunoprecipitation of specific antigens and preparation for SDS-PAGE
    http://www.sigmaaldrich.com/life-science/cell-biol...
    Added: Sat Jan 31 2009, Hits: 7044, Reviews: 0  Write review   Cached
  • Immunoprecipitation (Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
    Immunoprecipitation is a technique that permits the purification of specific proteins for which a antibody has been raised. This primary antibody is either already bound to agarose or can be bound to the protein A/agarose beads during the procedure in order to physically separate the antibody-antigen complex from the remaining sample. Two procedures are described including reagents used.
    http://www-ufk.med.uni-rostock.de/lablinks/protoco...
    Added: Sat Nov 02 2002, Hits: 3769, Reviews: 0  Write review   Cached
  • Metabolic labeling & Immunoprecipitation (Springer Lab, Harvard University)
    Includes radiolabeling and immunoprecipitation in great details.
    http://www.cbrinstitute.org/labs/springer/protocol...
    Added: Tue Aug 13 2002, Hits: 2439, Reviews: 0  Write review   Cached
  • Immunoprecipitation PDF file (Rosen Lab, Department of Molecular and Cellular Biology, Baylor College of Medicine)
    Formaldehyde cross-linking and chromatin immunoprecipitation assays of tissue culture cells
    http://public.bcm.tmc.edu/rosenlab/protocols/ChIP....
    Added: Sat Aug 24 2002, Hits: 5990, Reviews: 0  Write review  
  • Immunoprecipitation of Proteins (Upstate)
    Well written protocol from Upstate for general use.
    http://www.upstate.com/misc/protocols.q.prot.e.imm...
    Added: Tue Feb 24 2004, Hits: 13683, Reviews: 0  Write review   Cached
  • Immunoprecipitation with Soluble Antibodies (BD Biosciences)
    General protocol for immunoprecipittion with soluble antibodies including preparation of cell lysate, preclearing and immunoprecipitation.
    http://www.bdbiosciences.com/support/resources/pro...
    Added: Sun Aug 26 2012, Hits: 397, Reviews: 0  Write review   Cached
  • Chromatin Immunoprecipitation from Human Embryonic Stem Cells Video (Journal of Visualized Experiments)
    Chromatin immunoprecipitation involves the purification of in vivo cross-linked chromatin. The isolated chromatin is reduced to smaller fragments by enzymatic digestion or mechanical force. Chromatin fragments are precipitated using specific antibodies to target proteins or protein and DNA modifications. The precipitated DNA or RNA is purified and used as a template for PCR or DNA microarray based assays.
    http://www.jove.com/index/details.stp?ID=780
    Added: Mon Feb 09 2009, Hits: 2335, Reviews: 0  Write review   Cached
  • Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap (Epigenome Network of Excellence)
    This protocol, using the versatile GTP-Trap, can be used to perform immunoprecipitation of fluorescent fusion proteins from cell extracts to identify and map protein-protein interactions as exemplarily shown by the dimerization of Dnmt1
    http://www.epigenome-noe.net/researchtools/protoco...
    Added: Wed May 27 2009, Hits: 1021, Reviews: 0  Write review  
  • Immunoprecipitation (Hancock Lab)
    http://www.cmdr.ubc.ca/bobh/methods/IMMUNOPRECIPIT...
    Added: Tue May 14 2002, Hits: 4592, Reviews: 0  Write review   Cached
  • Immunoprecipitation of native chromatin: NChIP PDF file (Chromatin and Gene Expression Group, The University of Birmingham)
    NChIP procedure from the isolation of chromatin (from cultured cells), through the immunoprecipitation step and subsequent DNA and protein analysis. This method is used primarily for analysis of histones post-translationally modified at specific residues
    http://www.ii.bham.ac.uk/research/chromatin/docs/N...
    Added: Tue Jan 27 2009, Hits: 1825, Reviews: 0  Write review  
  • Immunoprecipitation and Immune Complex kinase assay (Sefton Lab, Salk Institute)
    http://pingu.salk.edu/~sefton/Hyper_protocols/ipPr...
    Added: Tue May 14 2002, Hits: 3141, Reviews: 0  Write review   Cached
  • Chromatin immunoprecipitation for Plant Materials (Plant Methods)
    A robust ChIP protocol, using maize (Zea mays) as a model system, and present a general strategy to systematically optimize this protocol for any type of tissue.
    http://www.plantmethods.com/content/3/1/11
    Added: Tue Jan 27 2009, Hits: 2752, Reviews: 0  Write review   Cached
  • Immunoprecipitation from Eukaryotic Cell Extracts (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
    http://www.pmci.unimelb.edu.au/core_facilities/man...
    Added: Tue May 14 2002, Hits: 1428, Reviews: 0  Write review   Cached
  • Tissue Chromatin Immunoprecipitation (ChIP) PDF file (Aviva Systems Biology)
    This short protocol is for the preparation of tissue samples for ChIP assay
    http://www.avivasysbio.com/corp/Tissue%20ChIP%20pr...
    Added: Tue Jan 27 2009, Hits: 5834, Reviews: 0  Write review  
  • Chromatin Immunoprecipitation (ChIP) Assay Protocol PDF file (Genome Center, UC Davis)
    Standard and verified ChIP protocol
    http://www.genomecenter.ucdavis.edu/expression_ana...
    Added: Tue Jan 27 2009, Hits: 8285, Reviews: 0  Write review  
  • Immunoprecipitation of metabolically labeled cells (Daniel T. O'Connor, UCSD Chromaffin Cell and Hypertension Research )
    http://hypertension.ucsd.edu/list_of_protocols/12_...
    Added: Tue May 14 2002, Hits: 1169, Reviews: 0  Write review   Cached
  • Chromatin Immunoprecipitation (ChIPs) Cloning Protocol (Farnham Laboratory, UC Davis)
    Cloning pulled down DNA from ChIP assay
    http://genomics.ucdavis.edu/farnham/protocols/ChIP...
    Added: Sat Jan 31 2009, Hits: 1321, Reviews: 0  Write review   Cached
  • Chromatin Immunoprecipitation Protocol to Analyze Histone Modifications in Arabidopsis Thaliana (Epigenome Network of Excellence)
    Plant cells have a rigid cell wall which poses limitations to the simple utilization of protocols established for animals. In this protocol, method used to study histone modifications in the plant model organism Arabidopsis thaliana is described.
    http://www.epigenome-noe.net/researchtools/protoco...
    Added: Tue Jan 27 2009, Hits: 917, Reviews: 0  Write review   Cached
  • Chromatin Immunoprecipitation Protocol for Saccharomyces Cerevisiae (Epigenome Network of Excellence)
    This protocol has been successfully used in the Gasser lab to study proteins at replication forks and chromosomal DNA double-strand breaks (DSBs) in budding yeast (Saccharomyces cerevisiae)
    http://www.epigenome-noe.net/researchtools/protoco...
    Added: Mon Jan 26 2009, Hits: 923, Reviews: 0  Write review   Cached
  • Yeast Chromatin Immunoprecipitation (ChIP) (The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
    Protocol for yeast ChIP in great details, may also used for other cell types.
    http://labs.fhcrc.org/hahn/Methods/mol_bio_meth/ha...
    Added: Sat Mar 27 2004, Hits: 2099, Reviews: 0  Write review   Cached
  • Plant Chromatin Immunoprecipitation PDF file (Craig Pikaard's Laboratory, Indiana University)
    http://sites.bio.indiana.edu/~pikaardlab/PDFs%20an...
    Added: Tue Mar 03 2009, Hits: 2131, Reviews: 0  Write review  
  • General Principles of Immunoprecipitation (Sefton Lab, Salk Institute)
    http://pingu.salk.edu/~sefton/Hyper_protocols/immu...
    Added: Tue Aug 13 2002, Hits: 5584, Reviews: 0  Write review   Cached
  • Chromatin Immunoprecipitation Protocol for Histone Modification & Chromatin Associated Proteins (Epigenome Network of Excellence)
    This protocol has been successfully used in experiments with Mouse Embryonic Stem (ES) cells, Embryonic Fibroblasts (MEF), Trophoblast Stem (TS) cells, B-lymphocytes and hepatocytes, as well as with U2OS, 293, Hela, T98G cells from human.
    http://www.epigenome-noe.net/researchtools/protoco...
    Added: Mon Feb 09 2009, Hits: 1142, Reviews: 0  Write review   Cached

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