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Category Matches: 7

• Cell Biology : Flow Cytometry (FCM) : Flow Cytomotry (FACS) of Tumor Tissues
• Cell Biology : Flow Cytometry (FCM) : PI Staining for FACS Analysis
• Cell Biology : Flow Cytometry (FCM) : Apoptosis Detection by FACS Analysis
• Cell Biology : Flow Cytometry (FCM) : Fluorescence-Activated Cell Sorting (FACS)
• Cell Biology : Flow Cytometry (FCM) : FACS Analysis Hematopoietic Cells
• Cell Biology : Flow Cytometry (FCM) : FACS Machine Operation and Settings
• Cell Biology : Flow Cytometry (FCM) : GFP Detection by FACS

Protocol Matches: 28

• Using FACS Scan Or FACS Calibur Machines (The Pamela Stanley Lab Wiki)
  Guide on how to use FACS machines
  http://stanxterm.aecom.yu.edu/wiki/index.php?page=...
  Added: Sat Jan 31 2009, Hits: 2916, Reviews: 0  Write review   Cached
• Standard Flow Cytometry (FACS) Protocol (Jackson Lab)
  FACS Analysis Using Peripheral Blood Cells
  http://www.jax.org/imr/facs.html
  Added: Tue May 14 2002, Hits: 8053, Reviews: 0  Write review   Cached
• FACS analysis of tumor tissues (Tang Laboratory, MD Anderson Cancer Center)
  Detailed protocol for FACS analysis of tumor tissues including tissue processing and marker staining.
  http://sciencepark.mdanderson.org/labs/tang/tumorp...
  Added: Wed Oct 24 2012, Hits: 229, Reviews: 0  Write review   Cached
• Yeast Prep for FACS Analysis (Gottschling Lab, Fred Hutchinson Cancer Research Center)
  http://labs.fhcrc.org/gottschling/Yeast%20Protocol...
  Added: Fri Jul 26 2002, Hits: 1583, Reviews: 0  Write review   Cached
• Cell Cycle Analysis by FACS (The Wolfson Institute for Biomedical Research)
  This is the best guide on how to do cell cycle analysis on a FACS machine. It gives step by step instructions of how to adjust settings on the machine (this PDF file also appears here http://www.icmb.utexas.edu/core/Microscopy/pdf/CELL_CYCLE_FUNDAMENTALS.pdf)
  http://www.ucl.ac.uk/wibr/services/docs/cellcyc.pd...
  Added: Sat Feb 14 2009, Hits: 4852, Reviews: 0  Write review  
• Cytokines for FACS Analysis (The Science Advisory Board)
  Protocol for intracytoplasmic staining of cytokines for FACS analysis
  http://www.scienceboard.net/resources/protocols.as...
  Added: Mon Feb 23 2009, Hits: 2367, Reviews: 0  Write review   Cached
• FACS Machine Instructions (Koshland Web Site/Methods)
  How to use FACS machine
  http://www.ciwemb.edu/labs/koshland/Protocols/YEAS...
  Added: Sat Feb 14 2009, Hits: 2372, Reviews: 0  Write review   Cached
• FACS Troubleshootings (Protocol Online)
  Archives of FACS troubleshootings
  http://www.protocol-online.org/biology-forums/flow...
  Added: Wed Feb 11 2009, Hits: 2648, Reviews: 0  Write review   Cached
• Complete Guide to Flow Cytometry (FACS) for Cell Cycle (Andrew Burgess, PhD)
  A complete Guide to Cell Cycle FACS, detailing how to fix, stain, aquire and analyze cells using a BD FACSCalibur and CellQuest software.
  http://sciencetechblog.files.wordpress.com/2011/05...
  Added: Wed May 25 2011, Hits: 2686, Reviews: 0  Write review  
• A Rapid & Quantitative Method for Detecting Apoptosis by FACS in Transiently Transfected Cells (Nucl. Acids Res)
  a method suitable for the flow cytometric determination of apoptosis in a large number of samples by propidium iodide (PI) in a population of cells transiently co-transfected with a plasmid encoding enhanced green fluorescent protein (eGFP) and a plasmid carrying a gene of interest. This assay combines the FACS-optimised GFP mutant (eGFP) as a marker to detect transiently transfected cells and the reduced fluorescence of the DNA-binding dye PI in the apoptotic subpopulation as a marker for apoptosis.
  http://nar.oxfordjournals.org/cgi/content/full/25/...
  Added: Fri Mar 20 2009, Hits: 1706, Reviews: 0  Write review  
• FACS Aria Start Up (Cell Sorter Facility, Yale University)
  http://info.med.yale.edu/immuno/cytometry/docs/FAC...
  Added: Mon Feb 23 2009, Hits: 1274, Reviews: 0  Write review   Cached
• Detection Of Cell Viability And/Or Apoptosis By Flow Cytometry (FACS) (The Pamela Stanley Lab Wiki)
  http://stanxterm.aecom.yu.edu/wiki/index.php?page=...
  Added: Sat Jan 31 2009, Hits: 4729, Reviews: 0  Write review   Cached
• Immunofluorescent Staining for FACS Analysis (Flow Cytometry Core Facility, PrincetonUniversity)
  General immunofluorescence staining protocol using directly and indirectly conjugated antibodies
  http://www.molbio1.princeton.edu/facility/flowcyt/...
  Added: Sat Feb 21 2009, Hits: 2568, Reviews: 0  Write review   Cached
• Preparing cells for PI/FACS (cell cycle) analysis (The Flemington Lab, Tulane Health Sciences Center)
  http://www.flemingtonlab.com/Protocols/PreparingCe...
  Added: Sat Jan 31 2009, Hits: 4806, Reviews: 0  Write review  
• FACS Analysis of ES Cells (NIH Stem Cell Information)  update
  http://stemcells.nih.gov/research/nihresearch/scun...
  Added: Thu Jan 29 2009, Hits: 1272, Reviews: 0  Write review   Cached
• Cell Sorting By FACS (The Pamela Stanley Lab Wiki)
  http://stanxterm.aecom.yu.edu/wiki/index.php?page=...
  Added: Sat Jan 31 2009, Hits: 8939, Reviews: 0  Write review   Cached
• PI Labeling Method for FACS Analysis (Chen Lab, UCSF)
  http://bts.ucsf.edu/chen/Protocol/PI%20Labeling%20...
  Added: Sun Oct 25 2009, Hits: 4985, Reviews: 0  Write review  
• Propidium Iodide Staining of Dead Cells For FACS Analysis (UCLA Flow Cytometry Core)
  http://cyto.mednet.ucla.edu/Protocols/pi.htm
  Added: Sat Jan 31 2009, Hits: 6007, Reviews: 0  Write review   Cached
• FACS Procedures for Apoptosis Detection (University of Pennsylvania,SDM Flow Cytometry Facility )
  Three methods are described: Hoechst vs. 7-AAD, TUNEL and Annexin V vs. 7-AAD
  http://biochem.dental.upenn.edu/~sdmfacs/apopproc....
  Added: Sat Jan 31 2009, Hits: 4962, Reviews: 0  Write review   Cached
• FACS Cell Cycle Staining using DAPI (Flow Cytometry Core Facility, University of Chicago)
  http://iacf.uchicago.edu/Protocols/DAPICellCyclePr...
  Added: Sat Feb 21 2009, Hits: 3267, Reviews: 0  Write review   Cached
• CD3/ CD4 /CD8 T Cell Subset Counts by FACS (The Science Advisory Board)
  Procedure for CD3/ CD4 /CD8 T cell subset counts using CD3-FITC / CD4-PE / CD8-PECy5 antibody
  http://www.scienceboard.net/resources/protocols.as...
  Added: Mon Feb 23 2009, Hits: 5290, Reviews: 0  Write review   Cached
• Use of BD FACS or Calibur Flow Cytometers (Fero Lab, Fred Hutchinson Cancer Research Center)
  Sample preparation, machine operation, parameter setting and data analyss
  http://labs.fhcrc.org/fero/Protocols/Flow.html
  Added: Sun Aug 01 2010, Hits: 1050, Reviews: 0  Write review   Cached
• Erythrocyte Lysing before FACS Analysis (UCSD Cancer Center Flow Cytometry Core Facility)
  http://cancer.ucsd.edu/flow/Protocols/rbclysing.as...
  Added: Sun Aug 01 2010, Hits: 697, Reviews: 0  Write review   Cached
• Sorting Preparation of Cells (UCSD Cancer Center Flow Cytometry Core Facility)
  How to prepare samples for FACS sorting.
  http://cancer.ucsd.edu/flow/Protocols/Sorting.asp
  Added: Sun Aug 01 2010, Hits: 1850, Reviews: 0  Write review   Cached
• Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells (Journal of Visualized Experiments)
  A video and text protocol for differentiating mouse ES cells including the following procedures: Assembling handing drops differentiation culture (EB formation), Collect undifferentiated ES cells, Assemble hanging drop culture, Collecting differentiated ES cells form hanging drops, Starting EB culture, EB dissociation and infection, Preparation of ES cell-derived progenitors for FACS and Transplantation.
  http://www.jove.com/index/Details.stp?ID=162
  Added: Wed Jan 28 2009, Hits: 1606, Reviews: 0  Write review   Cached

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