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- Autoradiography of Tissue Sections
(Gene Delivery Group, University of Birmingham)
Standard Protocol for autoradiography (35S)
http://web.bham.ac.uk/can4psd4/brum/auto.html
Added: Mon Sep 09 2002, Hits: 333, Reviews: 0 Write review Cached - Autoradiography and Intensifying Screens
( )
http://micro.nwfsc.noaa.gov/protocols/intense.html
Added: Tue May 14 2002, Hits: 719, Reviews: 0 Write review - Autoradiography (Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
This procedure involves the incorporation of a radioactive substance into a cell, and subsequent detection of that material through the use of a photographic emulsion. The primary source used for cell biology is an organic molecule containing tritium, the radioactive form of hydrogen. Radioactive carbon, phosphorous and iodine are occasionally utilized, but tritium has inherently more resolution than any of the others
http://homepages.gac.edu/~cellab/chpts/chpt2/ex2-8...
Added: Tue May 14 2002, Hits: 479, Reviews: 0 Write review Cached - Autoradiography and fluorography
(Sefton Lab)
http://pingu.salk.edu/~sefton/Hyper_protocols/ARG....
Added: Tue May 14 2002, Hits: 1289, Reviews: 0 Write review Cached - Southern Blot (Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
Localization of particular sequences within genomic DNA is usually accomplished by the transfer techniques described by Southern (1975). Genomic DNA is digested with one or more restriction enzymes, and the resulting fragments are separated according to size by electrophoresis through an agarose gel. The DNA is then denatured in situ and transferred from the gel to a solid support (usually nitrocellulose filter or nylon membrane). The relative positions of the DNA fragments are preserved during their transfer to the filter. The DNA attached to the filter is hybridized to radiolabeled DNA or RNA, and autoradiography is used to locate the positions of bands complementary to the probe.
http://www-ufk.med.uni-rostock.de/lablinks/protoco...
Added: Sat Nov 02 2002, Hits: 1665, Reviews: 0 Write review Cached -
Timing of Cycles
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
Determine Cell cycle by exposing cell to 3H-Thymidine, and subsequently fixing and autoradiography.
http://homepages.gac.edu/~cellab/chpts/chpt11/ex11...
Added: Tue May 14 2002, Hits: 1181, Reviews: 0 Write review Cached -
Timing of Cycles
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
Determine Cell cycle by exposing cell to 3H-Thymidine, and subsequently fixing and autoradiography.
http://homepages.gac.edu/~cellab/chpts/chpt11/ex11...
Added: Tue May 14 2002, Hits: 540, Reviews: 0 Write review Cached