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Protocol Matches: 7
- Anneal Oligonucleotides to Make Double-Stranded DNA
Added: Tue May 14 2002, Hits: 723, Reviews: 0
- Anneal Two Oligos to Make Double-Stranded DNA
It is sometimes necessary to make double stranded DNA from single stranded oligos. While the annealing procedure is very simple, attention to a few details can greatly reduce the presence of undesired single stranded material.
Added: Tue May 14 2002, Hits: 8401, Reviews: 0
- IN-Gel Hybridization procedure for telomeric single stranded DNA detection (Wellinger Lab, Université de Sherbrooke )
Telomeric DNA is replicated by a special mechanism in which an enzyme called telomerase will
elongate a short 3' overhang and create long, single stranded DNA at the ends of chromosomes. This enzyme requires a single stranded DNA portion as its substrate since the telomerase RNA moiety has to anneal with this DNA...
Added: Fri Jul 26 2002, Hits: 340, Reviews: 0
- Tail PCR Protocols (Preuss Lab, University of Chicago)
TAIL is a series of reactions that are intended to map where a T-DNA (transfer DNA) has inserted within the genome. The main components of the 3 reactions are the AD (Arbitrary Degenerate) primers, border primers, and DNA from the T-DNA lines that are to be mapped. AD primers are degenerate primers that anneal throughout the genome. The border primers are specific for the left and right borders of the T-DNA. From the primary reaction to the tertiary, the border primers get closer to the edge of the T-DNA. That is why a 'shift' is visible when running a gel with the secondary and tertiary reactions next to each other. The success rate of TAIL-PCR varies, depending onhow many DNA samples, AD primers, and border primers are used.
Added: Wed Feb 11 2009, Hits: 885, Reviews: 0
- PCR Mutagenesis Protocol (Michael Bumbulis, Baldwin-Wallace College)
PCR is based on the enzymatic amplification of a DNA fragment that is flanked by two oligonucleotide primers that anneal (stick) to opposite strands of the target sequence. The primers are oriented with their 3' ends pointing towards each other (recall that DNA is synthesized in the 5' to 3' direction). These primers are then extended by the polymerase enzyme. Repeated cycles of heat denaturation, annealing of the primers to their complementary sequences and extension of the primers result in the amplification of the segment defined by the 5' ends of the primers...
Added: Mon Feb 18 2013, Hits: 2010, Reviews: 0
EMSA Using Oligos
(Mike A. Dyer)
Anneal two complementary oligos to make ds oligos as the probe for EMSA assay.
Added: Tue May 14 2002, Hits: 2685, Reviews: 0
- Annealing Oligonucleotides
Method for making double-stranded oligonucleotides
Added: Sun Oct 26 2003, Hits: 6553, Reviews: 0