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Protocol Matches: 282
- PCR Box Titration Calculator
(Attotron Biosensor Corporation)
This JavaScript program calculates the amounts of various ingredients needed to do two-dimensional box titrations of various reactants in polmerase chain reactions.
http://www.attotron.com/pub/pcrtitr.htm
Added: Sat Jul 20 2002, Hits: 1454, Reviews: 0 Write review Cached -
Calculating Concentrations for PCR (Molecular Biology Techniques Manual)
How to calculate primer and nucleotide concentration
http://web.uct.ac.za/microbiology/pcrconcn.htm
Added: Tue May 14 2002, Hits: 5586, Reviews: 0 Write review Cached - PCR Guide
(LaboratoryExperiments.com)
Basic guide high school students, But very useful if you are new to molecular biology.
http://www.laboratoryexperiments.com/LAB2.htm
Added: Tue May 14 2002, Hits: 5988, Reviews: 0 Write review Cached - PCR from Bacterial Colonies
(Hideaki Shiraishi, Kyoto University)
This procedure is used to analyze inserts in pUC-derived plasimids.
http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protoc...
Added: Fri Jan 30 2004, Hits: 1239, Reviews: 0 Write review Cached - PCR Typing Ear Punches
(Case Transgenic and Targeting Facility)
http://transgenic.cwru.edu/protocols/pcrtypingearp...
Added: Sun Mar 22 2009, Hits: 252, Reviews: 0 Write review Cached -
Genome walking using splinkerette PCR (Cnidaria, University of California, Irvine)
An improvement on the vectorette approach is the so-called "splinkerette" method
http://www.ucihs.uci.edu/biochem/Steele/Splinkeret...
Added: Wed Feb 11 2009, Hits: 866, Reviews: 0 Write review -
Ligation-Mediated PCR for Genomic Sequencing & Footprinting
(The Morimoto Lab, Northwestern University)
http://www.biochem.northwestern.edu/morimoto/resea...
Added: Mon Mar 29 2004, Hits: 1032, Reviews: 0 Write review - PCR Guide
(Michael Blaber's Lab)
Practical and useful guide to selection of polymerase, primer design, and more...
http://wine1.sb.fsu.edu/bch5425/lect23/lect23.htm
Added: Tue May 14 2002, Hits: 1120, Reviews: 0 Write review Cached -
Inverse PCR (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
To Isolate DNA Adjacent To Known Sequence in Genomic DNA
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 800, Reviews: 0 Write review Cached - PCR on Worms
(Seydoux Lab)
http://www.bs.jhmi.edu/MBG/SeydouxLab/Protocols/Wo...
Added: Tue May 14 2002, Hits: 87, Reviews: 0 Write review Cached -
Purification of PCR products with Sephadex
(Vodkin Laboratory, University of Illinois)
http://soybeangenomics.cropsci.uiuc.edu/protocols/...
Added: Mon Aug 26 2002, Hits: 1607, Reviews: 0 Write review Cached - Inverse PCR (Gottschling Lab, Fred Hutchinson Cancer Research Center)
http://labs.fhcrc.org/gottschling/General%20Protoc...
Added: Tue Mar 03 2009, Hits: 10010, Reviews: 0 Write review Cached -
Detecting Transgenes by PCR and Southern Blot
(Case Transgenic and Targeting Facility)
http://transgenic.cwru.edu/protocols/dnacalc.html
Added: Sun Mar 22 2009, Hits: 617, Reviews: 0 Write review Cached -
Real-Timer PCR Primer Database
(Steven W. Johnson, Ph.D.)
A frequently updated database of validated primer/probe sets for quantitation of a large number of genes of various organisams.
http://www.realtimeprimers.org/
Added: Sat Mar 27 2004, Hits: 7643, Reviews: 0 Write review Cached - Quantitative Reverse-Transcription PCR (q-RT-PCR)
http://www.utexas.edu/research/sissonlab/protocols...
Added: Sat Jan 31 2009, Hits: 17564, Reviews: 0 Write review -
Degenerate PCR (Michael Koelle)
http://www.dartmouth.edu/~ambros/protocols/other/k...
Added: Tue May 14 2002, Hits: 1848, Reviews: 0 Write review Cached - PCR Genotyping of Embryos and Blastocysts
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 72, Reviews: 0 Write review Cached -
Sensitive mRNA Quantification - Gene Quantification Page
(Institute of Physiology)
Main focus of the GENE QUANTIFICATION web page is, to describe and summarize all technical aspects involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. e.g. detection chemistry, assay specificity, sensitivity, reproducibility, robustness, intra- & inter-assay variations, kinetic PCR efficiency calculation, quantification strategies, optimization strategies, data aquisition, data calculation, data normalisation, and statistics. To obtain high fidelity, accuracy and reliability in reverse transcription and subsequent kinetic PCR highly defined reliable quantification strategies, calculation procedures and statistical models are needed.
http://www.wzw.tum.de/gene-quantification/index.sh...
Added: Fri Jan 16 2004, Hits: 4831, Reviews: 0 Write review -
3% Trevigel Protocol for 3 bp resolution of SSR PCR products
(Kim Marshall)
http://dendrome.ucdavis.edu/Protocols/3p.html
Added: Tue May 14 2002, Hits: 130, Reviews: 0 Write review Cached -
Incorporation of Digoxigenin-dUTP into Plasmid Inserts Using PCR (CIMMYT Applied Molecular Genetics Laboratory)
http://www.cimmyt.org/english/docs/manual/protocol...
Added: Mon Aug 05 2002, Hits: 838, Reviews: 0 Write review -
Reverse Ligation Mediated RT-PCR (Antisense Research Group)
RL-PCR can be broken down into a number of simple steps. Synthesis by in vitro transcription and purification of an RNA linker species. Extraction of total RNA from cells into which oligodeoxynucleotide has been delivered by, for example, streptolysin O permeabilization. Ligation of the RNA linker to all available 5' monophosphates in the purified total cellular RNA sample. Reverse Transcription of the RNA using a gene - specific primer. Amplification of specific fragments by PCR using linker - specific and gene - specific primers. Sub- amplification of the first PCR product using linker specific and nested, labelled, gene specific primers.
http://www.liv.ac.uk/~giles/methods/rlpcr.htm
Added: Tue May 14 2002, Hits: 1694, Reviews: 0 Write review - Detection of Viruses in Infected Plant Extracts using Immunocapture-PCR (Protocol Online)
Diagnostic PCR should fulfill several qualitative characteristics, but the most important among them are specificity, sensitivity, efficiency and reproducibility of detection of viruses. Immunocapture-PCR (IC-PCR) may be used in detecting viruses using clarified plant extracts with degenerate primers, without prior isolation of total nucleic acids. It is especially useful for detecting viruses that exist in low or variable titers en planta and in plant species which contain various forms of PCR amplification inhibitors (polysaccharides, tannins, polyphenolics etc.).
Added: Mon Feb 02 2009, Reviews: 0 Write review -
In Situ
( )
PCR Cookbook (Katherine A. Staskus)
Detailed protocol for in situ PCR on tissue section
http://www.cbc.umn.edu/VirtLibrary/Staskus/germany...
Added: Tue May 14 2002, Hits: 635, Reviews: 0 Write review Cached - Reverse transcriptase (RT)-PCR (Langdale Lab, Department of Plant Sciences, University of Oxford)
Protocol describes DNase treatment, the RT reaction and PCR.
http://dps.plants.ox.ac.uk/langdalelab/protocols/P...
Added: Wed Feb 11 2009, Hits: 23019, Reviews: 0 Write review - Enzyme-Free Cloning (Daniel Tillett and Brett A. Neilan, Nucleic Acids Research)
Describes a simple method for the cloning of PCR products without the need for post-amplification enzymatic treatment. Tailed PCR primer sets are used to create complementary staggered overhangs on both insert and vector by a post-PCR denaturation–hybridisation reaction. The single-stranded overhangs are designed to allow directional cloning in a ligase-free manner. This ‘enzyme-free cloning’ procedure is highly efficient, and is not constrained by the need for the presence of suitable restriction enzyme sites within the plasmid vector. The avoidance of post-amplification enzymatic procedures makes the technique rapid and reliable, avoiding the need for multiple sub-cloning steps.
http://nar.oxfordjournals.org/cgi/content/full/27/...
Added: Sun Oct 25 2009, Hits: 1880, Reviews: 0 Write review