Protocol Online: Standard Operating Procedures for Phage Testing Assay

Standard Operating Procedures for Phage Testing Assay

Source: BACPAC Resource Center (BPRC) at Children's Hospital Oakland Research Institute in Oakland
Abstract: This assay uses a lawn of phage-susceptible E. coli (DH10B) embedded in a layer of agarose. This top agarose lays on a bed of standard LB agar. If phage is present in a clone streaked or stamped onto the top agarose lawn, the lawn there will be lysed on incubation overnight.
Date Added: 2009-02-11
Date Modified: 2009-02-11