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Documents 81 - 90 of 143 matches.
Dialysis - Protein Expression and Purification**
http://www.protocol-online.org/biology-forums-2/posts/14329.html
... after incubating with E. coli lysis of His tagged protein. I don't know if it is because the sonicated E. coli lysis is too cencontrated (100ml medium of E. coli sonicated in 7ml buffer) or because ...
Primer optimization for ChIP - ChIP and Next Generation Sequencing**
http://www.protocol-online.org/biology-forums-2/posts/11385.html
... DNA as a template for the PCR reaction, however, that's not the case when I use 20% Input of sonicated DNA as a template. I don't think that it's a problem with the quality of sheared DNA ...
ChIP-Chip vs. ChIP-Seq - ChIP and Next Generation Sequencing**
http://www.protocol-online.org/biology-forums-2/posts/13954.html
... -on-Chip and ChIP-Seq are, respectively? I guess the resolution will be different, but when using sonicated chromatin, does this actually really factor in that much? Are there any other pitfalls (e.g. ...
Sterilization - Cell Biology**
http://www.protocol-online.org/biology-forums-2/posts/11600.html
... in emulsion form and it is difficult to sterilize by filtration i m thinking of sterilizing it by sonication (water bath) any suggestion on that z0z -z0z- well that can be fine but check if your protein ...
How Best to Clean the Sonicator Tip Between Samples? - SDS-PAGE and Western Blotting*
http://www.protocol-online.org/biology-forums-2/posts/11195.html
... -p...11124bc743356ff -lab rat- I clean it between samples with the same buffer i am using to sonicate. And after that with ethanol -laurequillo- Envennom on Oct 28 2009, 11:43 PM said:Hi! Can someone ...
Is it possible to make 10M NaCl? - General Lab Techniques*
http://www.protocol-online.org/biology-forums/posts/15500more1.html
... ) Pages: Previous 1 2  6M Ammonium Acetate -John Buckels- what happen under high pressure? and is sonication usefull in this case? Seb -tryptofan- I think this is simply a case of companies making assumptions ...
Problem clariflying/filtering insect cell lysate - Protein Expression and Purification*
http://www.protocol-online.org/biology-forums-2/posts/15456.html
... buffer (~1L culture in 100mls of buffer) I pipette them up and down to disburse the cells and sonicate briefly to break cells. I then spin down the mixture which removes most cell debris and tried filtering ...
Problem with Protein elution using GST sepharose 4B - Protein Expression and Purification*
http://www.protocol-online.org/biology-forums-2/posts/10297.html
... ml lyzozyme and 1X protease inhibitor cocktail (sigma) 3) Incubate at 37oC for about one hour. 4) Sonicate the cells at 32% amplititude for 10 secs at intervals of 30 sec for 40 secs. 5) Spin down at ...
ChIP in WT and KO cells - how to analyze - ChIP and Next Generation Sequencing*
http://www.protocol-online.org/biology-forums-2/posts/12934.html
... WT + inducer KO KO + inducer My problem is that I don't know how to properly compare them. I sonicated and did ChIP all on the same day, so they are from the same experiment. Should I use percent ...
SAMPLE MIGRATION PROBLEM IN sds PAGE - SDS-PAGE and Western Blotting*
http://www.protocol-online.org/biology-forums-2/posts/10474.html
... . you can see the weird shape of the sample after migration. so what is it? the sample were sonicated many time (so no DNA). protein loaded was 50ug. any help ' href="http://www.protocol-online.org/forums ...

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