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Documents 61 - 70 of 143 matches.
IPTG Induction and cell lysis issues - SDS-PAGE and Western Blotting**
http://www.protocol-online.org/biology-forums-2/posts/14988.html
... , I have attempted 1.0mM at 22, 30, and 37 celsius for 4, 6, 8 hours. For my lysis I have used sonication and also have tried B-PER Bacterial Protein Extraction Reagent (Thermo) using standary protocols ...
cleaning sonicator tip to prevent DNA contaminationin samples - DNA Methylation and Epigenetics**
http://www.protocol-online.org/biology-forums-2/posts/13319.html
... everything I've read, I'm concerned that the Aquasonic would not produce the frequency of sonication necessary to shear the DNA (the manual says it produces 50-60 Hz). If anyone has used this ...
bacteria lysis - Protein and Proteomics**
http://www.protocol-online.org/biology-forums-2/posts/10800.html
... get the same type of cell lysis? -NK22- Hi, I do the bacteria lysis with PBS and tween20+ DTT and sonication. Some time I add sarcosyl. But the lysis buffer is PBS-T. DTT or sarcosyle is for solubility ...
can a pellet be called "probe"? - General Lab Techniques**
http://www.protocol-online.org/biology-forums/posts/14703more1.html
... ....but when i try to solubilize my protein into the buffer i fail... sad.gif i tried lysozyme, sonication different buffers......but up till now no progress..... sad.gif it just seems so insoluble... ...
[10078.html]**
http://www.protocol-online.org/biology-forums/posts/10078.html
... processing for WBs what is the difference of the resultant protein sample based on whether I sonicate or homogenize? Thanks! -jasonrc- USE SONICATION IN 0.1 M NaCl FOR 15 MIN -sasu- QUOTE (jasonrc ...
RIPA buffer pH? - Protein and Proteomics**
http://www.protocol-online.org/biology-forums-2/posts/10358.html
... one is the most suitable for mammalian cell culture (like HeLa, HepG2 etc)? After that, should sonication be performed again? Lastly, will spin down the cell lysate at 13k rpm for 5 minutes sufficient ...
How to incorporate dUTP when using WGA2 amplification for Affymetrix’s array? - ChIP and Next Generation Sequencing**
http://www.protocol-online.org/biology-forums-2/posts/11735.html
... Chip-chip you shouldn't have to do the fragmentation step of the WGA as your DNA is already be sonicated. We use the Sigma WGA kit and just go straight to adding the library prep buffer etc after ...
Why Ni-NTA beads became clumped after incubation? - Protein Expression and Purification**
http://www.protocol-online.org/biology-forums-2/posts/15137.html
... after incubating with E. coli lysis of His tagged protein. I don't know if it is because the sonicated E. coli lysis is too cencontrated (100ml medium of E. coli resuspened and sonicated in 7ml buffer ...
direct lysis of cell pellet - SDS-PAGE and Western Blotting**
http://www.protocol-online.org/biology-forums-2/posts/15964.html
... :y cant u heat becos of the urea? urea will decompose and carbamylate the protein. -mdfenko- try sonication. 30s. good luck. -redhill_2000-
Bradford quantitatn problem - precipitation - Protein and Proteomics**
http://www.protocol-online.org/biology-forums-2/posts/11505.html
... topic in live forum   Printer Friendly Version Hi, I extracted my protein using 2%SDS (scraping, sonication). It precipitate out as blue particle when i added into my bio-rad protein assay reagent. It ...

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