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very low ChIP'd DNA amount. - ChIP and Next Generation Sequencing**
http://www.protocol-online.org/biology-forums-2/posts/12174.html
... of ChIP'd DNA? And I noticed that SDS in my samples kept being precipitating down during my sonication step. Is it normal? Oh~~~~, ChIP is torturing me!! Thank you very much for your replies ...
How long samples can be stored - ChIP and Next Generation Sequencing**
http://www.protocol-online.org/biology-forums-2/posts/14899.html
... ? or just corsslinked cells? My plan was just to store crosslinked cells and use them later for sonication and IP. -epigenetics- epigenetics on May 18 2010, 10:09 AM said: KPDE on May 18 2010, 09& ...
Inconsistent results from replicates in Taqman real-time PCR - Real-Time PCR**
http://www.protocol-online.org/biology-forums/posts/17599more1.html
... least above to 10 pre reaction… -rye- at 4C - check pH - freezing at -80 C - overnight helps - sonication helps to shift to lower Ct smile.gif but after that do not QUANTIFY - sonicated vs nonsonicated ...
no PCR product from input... - ChIP and Next Generation Sequencing**
http://www.protocol-online.org/biology-forums-2/posts/15878.html
... the ChIP, but input does it was with 1 well of ES cells from a 6-well plate, using bioruptor for sonication, 1ml solution then I went on to repeating it, but this time i used 3 wells of cells, in 2 ml ...
DNA shearing optimization for ChIP assay - DNA Methylation, Histone and Chromatin Study**
http://www.protocol-online.org/biology-forums/posts/14857.html
... in 2% agarose gel, but had no idea about how to interpret the result and how to modify the sonication condition next time. So I hope that maybe someone can give me some hints. The picture of gel has been ...
High background with biorupter - ChIP and Next Generation Sequencing**
http://www.protocol-online.org/biology-forums-2/posts/13565.html
... , after switching to the Bioruptor, in the size of the residual pellet when centrifuging after sonication. -KPDE- Hello annabella, this is just a wild guess as I can't see a good reason why you would ...
Baculovirus Expression - Cell Biology**
http://www.protocol-online.org/biology-forums/posts/11350.html
BACULOVIRUS EXPRESSION - SONICATE INFECTED CELLS? (NOV/19/2005 ) I'm infecting Sf9 cells with a bacmid and am about to harvest the initial transfection. I am wondering if I would make sense to sonicate ...
Troubleshooting GST purification - Protein Expression and Purification**
http://www.protocol-online.org/biology-forums-2/posts/11382.html
... Friendly Version I'm trying to purify a GST tagged protein. We see expression in the lysate, sonicate, but when we incubate with GST beads, we lose everything. We see non specific proteins in the ...
DNA extration from Bacteria spores - Microbiology**
http://www.protocol-online.org/biology-forums-2/posts/13038.html
... to isolation genomic DNA from Bacteria spores without much success. We used various procedures (sonication, beat beating, heating, microwaving, bleach treatment) and we also tried different combinations ...
my western blots of membrane protein do not work - General Lab Techniques**
http://www.protocol-online.org/biology-forums-2/posts/13358.html
... what is the step in which the total extract was degradated. i solubilise on ripa, on ice, sonicate, centrifuge at 4°c, dnase at 37°c, add loading bufer,boil and load!! rrun, the gel, -luciana ...

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