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Documents 31 - 40 of 143 matches.
How can I decrease IgG background? - ChIP and Next Generation Sequencing**
http://www.protocol-online.org/biology-forums-2/posts/14067.html
... . Vigorously resuspend cell pellets in 300μl lysis buffer, incubate on ice for 10 minutes. 7. Sonicate for 15s*3 times at 45% output, with 1 minutes refractory period between sonications 8. Centrifugate ...
Lysing hela cells - Cell Biology**
http://www.protocol-online.org/biology-forums/posts/16799more1.html
... how long do you incubate cells with lysis buffer? You cannot simply vortex immediatly unless you sonicate your samples. You can use different amount of detergent ranging from 0.1% to 1% but the lower ...
[10035more1.html]**
http://www.protocol-online.org/biology-forums/posts/10035more1.html
PCR AFTER SONICATION... - (OCT/04/2005 ) Pages: Previous 1 2  I didnt really change anything. It still workes eventhough the cells arent lysed b4 I sonicate. I just get more of a smear compaired to no ...
To sonicate a large volume of bacterial cells - Microbiology**
http://www.protocol-online.org/biology-forums/posts/14168.html
TO SONICATE A LARGE VOLUME OF BACTERIAL CELLS - ANY BETTER METHOD? (MAR/02/2006 ) I need quite a lot of my bacteria. Every time, I will grow around 15L of cells and do protein extraction by sonication ...
How can I decrease IgG background? - ChIP and Next Generation Sequencing**
http://www.protocol-online.org/biology-forums-2/posts/14067more1.html
... . Vigorously resuspend cell pellets in 300μl lysis buffer, incubate on ice for 10 minutes. 7. Sonicate for 15s*3 times at 45% output, with 1 minutes refractory period between sonications 8. Centrifugate ...
Expression in E.coli - Protein Expression and Purification**
http://www.protocol-online.org/biology-forums-2/posts/15618more1.html
... . -bhavana- This is how I do it: harvest 100 ml culture re-suspend pellet in 5ml lysis buffer, sonicate supernatant: soluble fraction pellet: re-suspend in 500 ul lysis buffer: insoluble fraction Use 1 ...
additional proteins in eluted protein-protein purification of GST tag fusion pro - Protein Expression and Purification**
http://www.protocol-online.org/biology-forums-2/posts/14742.html
... resuspended pellet 5mM dithiotheritol (DTT), 1mM EDTA and 0.25% sarkosyl added. The solution is sonicated 4 times (10secs of sonication with 10secs of cooling ice), speed 2 with Branson sonifier and centrifuged ...
SDS-PAGE - Protein and Proteomics**
http://www.protocol-online.org/biology-forums/posts/1330more1.html
... what protocol seems to give good clean lysate with SDS lysis buffer (re, specific steps such as sonication or heating etc). cheers! sean. -smath- I think the protein concentration of your sample may ...
Spores separation from fermentation broth - General Lab Techniques**
http://www.protocol-online.org/biology-forums-2/posts/15827.html
... be greatly appreciated. -digestion- supposing you are working with fungal spores I would try (i) sonication or (ii) adding some mild detergent. For most fungi this should work (if the spores are not already ...
Fragment sizes after ChIP - ChIP and Next Generation Sequencing**
http://www.protocol-online.org/biology-forums-2/posts/14714.html
... have immunoprecipitated and I observe 2 smears, one at 100/250 pb and one at 1000/10000 pb. After sonication I have only a smear around 200bp (on agarose gel). So I wonder what is this second smear. Is ...

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