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Documents 21 - 30 of 143 matches.
Fast Decrosslinking - ChIP and Next Generation Sequencing**
http://www.protocol-online.org/biology-forums-2/posts/14622.html
... Version Hello, I was wondering if anyone had a relatively fast way to decrosslink following sonication. I like to check the size of my chromatin before I proceed to the IP, but I currently use proteinase ...
SDS-PAGE - Protein and Proteomics**
http://www.protocol-online.org/biology-forums-2/posts/1330more1.html
... what protocol seems to give good clean lysate with SDS lysis buffer (re, specific steps such as sonication or heating etc). cheers! sean. -smath- I think the protein concentration of your sample may ...
Samples for ChIP-seq library preparation - ChIP and Next Generation Sequencing**
http://www.protocol-online.org/biology-forums-2/posts/14445.html
... a lot of small fragments at 100/150pb on my gel, even should be at about 250bp (about 200pb after sonication), degradation ?). For the AMPure beads (I used AMPure and not AMPure XP), I used a lot of beads ...
Weak Western band for phosphorylated Akt - Protein and Proteomics**
http://www.protocol-online.org/biology-forums/posts/10675.html
... ) Pages: 1 2 Next Hi Guys, I have been doing westerns with P-akt for a while now. Earlier I used to sonicate my cells and now I have switched to cell lysis without sonication. I am not sure but these days ...
sonication of PBMC - Immunology and Histology**
http://www.protocol-online.org/biology-forums/posts/1346.html
SONICATION OF PBMC - (FEB/05/2001 ) sir, I am in desperate need of a protocol for the sonication of PBMC for animportant experiment, where I would ultimately do a Western Blot with the protein extract ...
Ponceau S and Coomasie do not correlate to b actin signal - SDS-PAGE and Western Blotting**
http://www.protocol-online.org/biology-forums-2/posts/14857.html
... I use for in vitro purposes and of course for my protein of interest and then the samples were sonicated as usual. The supernatant was kept and the protein assay was performed as usual. The triplicates ...
ChIP sonication Input Agarose gel - DNA Methylation, Histone and Chromatin Study**
http://www.protocol-online.org/biology-forums/posts/10671.html
CHIP SONICATION INPUT AGAROSE GEL - (OCT/26/2005 ) Hi, I'm having an odd experience with Agrose gel electrophoresis. After my ChIP sonication, I aliquot a 100ul out, did the reverse crosslink, phenol ...
LPS isolation by hot phenol\water method - Microbiology**
http://www.protocol-online.org/biology-forums/posts/13636more1.html
... ? If someone is just interested in extracting lipophilic LPS, would you recommend going through sonication/ enzyme treatment etc? -genehunter-1- QUOTE (genehunter-1 @ Aug 15 2007, 10:25 AM ...
Lysing hela cells - Cell Biology**
http://www.protocol-online.org/biology-forums/posts/16799more1.html
... how long do you incubate cells with lysis buffer? You cannot simply vortex immediatly unless you sonicate your samples. You can use different amount of detergent ranging from 0.1% to 1% but the lower ...
[10035more1.html]**
http://www.protocol-online.org/biology-forums/posts/10035more1.html
PCR AFTER SONICATION... - (OCT/04/2005 ) Pages: Previous 1 2  I didnt really change anything. It still workes eventhough the cells arent lysed b4 I sonicate. I just get more of a smear compaired to no ...

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