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Documents 21 - 30 of 143 matches.
Fast Decrosslinking - ChIP and Next Generation Sequencing**
http://www.protocol-online.org/biology-forums-2/posts/14622.html
... Version Hello, I was wondering if anyone had a relatively fast way to decrosslink following sonication. I like to check the size of my chromatin before I proceed to the IP, but I currently use proteinase ...
SDS-PAGE - Protein and Proteomics**
http://www.protocol-online.org/biology-forums-2/posts/1330more1.html
... what protocol seems to give good clean lysate with SDS lysis buffer (re, specific steps such as sonication or heating etc). cheers! sean. -smath- I think the protein concentration of your sample may ...
Dual cross-linking thoughts - ChIP and Next Generation Sequencing**
http://www.protocol-online.org/biology-forums-2/posts/12734more1.html
... lowering the SDS concentration can improve the efficiency of the ChIP? create less bubbles during sonication step? I used Upstate ChIP kit. I tried DTBP before. It will enrich the DNA to which the protein ...
Samples for ChIP-seq library preparation - ChIP and Next Generation Sequencing**
http://www.protocol-online.org/biology-forums-2/posts/14445.html
... a lot of small fragments at 100/150pb on my gel, even should be at about 250bp (about 200pb after sonication), degradation ?). For the AMPure beads (I used AMPure and not AMPure XP), I used a lot of beads ...
Weak Western band for phosphorylated Akt - Protein and Proteomics**
http://www.protocol-online.org/biology-forums/posts/10675.html
... ) Pages: 1 2 Next Hi Guys, I have been doing westerns with P-akt for a while now. Earlier I used to sonicate my cells and now I have switched to cell lysis without sonication. I am not sure but these days ...
purity is so bad after elution from GST column - Protein and Proteomics**
http://www.protocol-online.org/biology-forums-2/posts/14634.html
... the proteins, though not all. Also, the DTT should be added before you lyse the cells (even before sonication). Another alternative is to tag both termini of the protein and do a double purification. ...
sonication of PBMC - Immunology and Histology**
http://www.protocol-online.org/biology-forums/posts/1346.html
SONICATION OF PBMC - (FEB/05/2001 ) sir, I am in desperate need of a protocol for the sonication of PBMC for animportant experiment, where I would ultimately do a Western Blot with the protein extract ...
Ponceau S and Coomasie do not correlate to b actin signal - SDS-PAGE and Western Blotting**
http://www.protocol-online.org/biology-forums-2/posts/14857.html
... I use for in vitro purposes and of course for my protein of interest and then the samples were sonicated as usual. The supernatant was kept and the protein assay was performed as usual. The triplicates ...
ChIP sonication Input Agarose gel - DNA Methylation, Histone and Chromatin Study**
http://www.protocol-online.org/biology-forums/posts/10671.html
CHIP SONICATION INPUT AGAROSE GEL - (OCT/26/2005 ) Hi, I'm having an odd experience with Agrose gel electrophoresis. After my ChIP sonication, I aliquot a 100ul out, did the reverse crosslink, phenol ...
LPS isolation by hot phenol\water method - Microbiology**
http://www.protocol-online.org/biology-forums/posts/13636more1.html
... ? If someone is just interested in extracting lipophilic LPS, would you recommend going through sonication/ enzyme treatment etc? -genehunter-1- QUOTE (genehunter-1 @ Aug 15 2007, 10:25 AM ...

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