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Documents 11 - 20 of 143 matches.
do you sonicate or digest with lysozyme? - Protein and Proteomics**
http://www.protocol-online.org/biology-forums/posts/14407more1.html
DO YOU SONICATE OR DIGEST WITH LYSOZYME? - WHICH ONE DO YOU PREFER AND WHY.... (MAR/08/2006 ) Pages: Previous 1 2 3 Next wait a minute, Kathy so, if I am getting this straight, you are sonicating, spinning ...
White Precipitate Following Sonication - ChIP and Next Generation Sequencing**
http://www.protocol-online.org/biology-forums-2/posts/12019.html
WHITE PRECIPITATE FOLLOWING SONICATION - (DEC/08/2009 ) Visit this topic in live forum   Printer Friendly Version I had a quick question. I'm still a relative newbie at ChIP and want to ensure that ...
RNase treatment before analyzing sonicated chromatin in ChIP - DNA Methylation and Epigenetics**
http://www.protocol-online.org/biology-forums-2/posts/11023.html
RNASE TREATMENT BEFORE ANALYZING SONICATED CHROMATIN IN CHIP - (OCT/21/2009 ) Visit this topic in live forum   Printer Friendly Version Dear All, I just entered in the world of ChIP, and I am currently ...
Has anyone tried to sonicate Jurkat cells for ChIP assay? - DNA Methylation, Histone and Chromatin Study**
http://www.protocol-online.org/biology-forums/posts/10676.html
HAS ANYONE TRIED TO SONICATE JURKAT CELLS FOR CHIP ASSAY? - (OCT/26/2005 ) Hi, I'm having problems with sonication for Jurkat cells. I did try couple methods like, MNase treated (I did titration ...
low total/input DNA levels in ChIP work-up - ChIP and Next Generation Sequencing**
http://www.protocol-online.org/biology-forums-2/posts/11664.html
... can you make the initial re-suspension of pellet in IP buffer without incurring problems? My sonication step isn't optimal; could this also be contributing to the aparrent low levels of DNA? Also ...
do you sonicate or digest with lysozyme? - Protein and Proteomics**
http://www.protocol-online.org/biology-forums/posts/14407.html
DO YOU SONICATE OR DIGEST WITH LYSOZYME? - WHICH ONE DO YOU PREFER AND WHY.... (MAR/08/2006 ) Pages: 1 2 3 Next i have already posted this type of question before, but this time i want to know whether ...
Do you sonicate before adding to GST beads (slurry)? - Protein and Proteomics**
http://www.protocol-online.org/biology-forums/posts/13499.html
DO YOU SONICATE BEFORE ADDING TO GST BEADS (SLURRY)? - (FEB/07/2006 ) molecular cloning says dont sonicate....but some papers use sonication....what do you usually do? thanx a lot -Kathy- QUOTE (Kathy ...
What color should the cell pellets be after sonication and centrifugation? - DNA Methylation and Epigenetics**
http://www.protocol-online.org/biology-forums-2/posts/12880.html
WHAT COLOR SHOULD THE CELL PELLETS BE AFTER SONICATION AND CENTRIFUGATION? - (JAN/28/2010 ) Visit this topic in live forum   Printer Friendly Version Hi, this is really a useful forum, and there is a ...
Protein Expression in stationary phase - SDS-PAGE and Western Blotting**
http://www.protocol-online.org/biology-forums-2/posts/13894.html
... detergents, which are used for lysis. You either need a different type of assay (BCA, etc) or use sonication instead of chemical lysis for whole cell lysate preparation. The sonication buffer can be formulated ...
[10035.html]**
http://www.protocol-online.org/biology-forums/posts/10035.html
PCR AFTER SONICATION... - (OCT/04/2005 ) Pages: 1 2 Next Hi, I'm just trying to set everything up before I do the immunoprecipitation. I did a PCR after Sonication (500bp average) and tried to amplify ...

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