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Documents 91 - 100 of 143 matches.
RIPA and bacteria - General Lab Techniques*
http://www.protocol-online.org/biology-forums-2/posts/12735.html
... thought was to use RIPA buffer to lyse the macrophages, then after centrifugation of the lysate, sonicate the pellet, which should include bacterial proteins. I am not sure, however, that RIPA will not ...
Whole cell lysate including nucelar proteins? - Protein and Proteomics*
http://www.protocol-online.org/biology-forums-2/posts/13193.html
... you should do a SDS lysis. Just boil the samples for 2min in SDS buffer (laemmli buffer) 1x, the sonicate 2x 20sec and then boil again 5min. -laurequillo-
Processing cell samples for low abundance proteins - SDS-PAGE and Western Blotting*
http://www.protocol-online.org/biology-forums-2/posts/15521.html
... You can freeze the pellets at -80 (without PBS or SDS), or you can add 1x SDS, boil it for 2 min, sonicate, and boil again for 5 min and then load the gels or freeze the samples. Just be careful if you ...
ChIP - no immunoprecipitated DNA - DNA Methylation, Histone and Chromatin Study*
http://www.protocol-online.org/biology-forums/posts/12284.html
... .I am very puzzling,please help me .the main procedure is following: First,i'm sure that the sonication is no problem.The sonicated-chromatin was about 300-400bp,and i proved it in an agarose gel.Second ...
E coli lysis by lysozyme - Protein Expression and Purification*
http://www.protocol-online.org/biology-forums-2/posts/16009.html
... too large so they flow around in the solution and contribute to not 100% clear In comparison with sonication, I always get very very clear lysate. Lysozme treatment can only best give me lysate ~50% clear ...
How to test ChIP cross-linking and reversal? - DNA Methylation, Histone and Chromatin Study*
http://www.protocol-online.org/biology-forums/posts/17263.html
... 1, 179-185). This method is robust, involves MUCH less labor (we can complete the assay, from sonicated chromatin to PCR ready DNA, in about 3.5 to 5 hours for 20-24 samples) and has replaced the traditional ...
How strong is the "passive absorption"interaction? - Protein and Proteomics*
http://www.protocol-online.org/biology-forums-2/posts/10088.html
... passive absorption (by different charges), how strong will be their interaction? Reversible? If i sonicate them with a ultrasonic bath, will it dissociate them? -LOW- LOW on Sep 3 2009, 12:22 AM said ...
problem in my gluthatione seharose beads? - Protein and Proteomics*
http://www.protocol-online.org/biology-forums/posts/17118more1.html
... u use? -spanishflower- Sorry, we have not used bugbuster lysis buffer for the high price, we use sonication. QUOTE (spanishflower @ Jun 27 2006, 07:44 PM) hi guys thankx i have a question , have ...
Weak Western band for phosphorylated Akt - Protein and Proteomics*
http://www.protocol-online.org/biology-forums/posts/10675more1.html
... ) Pages: Previous 1 2  Hi Pria, Just a thought but given your differences in yields between your sonication and tissue lysis, is your Bradford assay compatible to the detergent in the lysis buffer??? ...
Sound and plants - Botany and Plant Biology*
http://www.protocol-online.org/biology-forums/posts/15379more1.html
... changes in protein structure and thus function, e.g. very high frequencies can degrade proteins (sonication) -grapes of wrath- thanx! that's a new concept.. something new to think about. biggrin ...

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