BioForum Archieves Search Results for 'sonication'

Search results for '(sonication or sonicate or sonicated)'

Documents 1 - 10 of 143 matches.

DNA sonication - RNA contamination? - ChIP and Next Generation Sequencing: DNA SONICATION - RNA CONTAMINATION? - NEW PROBE, START ALL OVER (MAR/17/2010 ) Visit this topic in live forum Printer Friendly Version Pages: 1 2 Next OK so I am using a new probe with my Branson 450 digital sonicator. Turned out I was using the probe meant for, shall we say, slightly larger volumes ...
http://www.protocol-online.org/biology-forums-2/posts/13784.html 05/24/13, 17489 bytes

Sonication trouble - ChIP and Next Generation Sequencing: SONICATION TROUBLE - USING BOTH MNASE AND SONICATION TO GET 200-1000 BP (DEC/07/2009 ) Visit this topic in live forum Printer Friendly Version Hi, This is my very first post :lol: . I am working on Mouse Granulosa cells and we have been planning to do ChIP-CHIP. Our lab protocol combines both Mnase ...
http://www.protocol-online.org/biology-forums-2/posts/11991.html 05/24/13, 13882 bytes

DNA sonication - RNA contamination? - ChIP and Next Generation Sequencing: DNA SONICATION - RNA CONTAMINATION? - NEW PROBE, START ALL OVER (MAR/17/2010 ) Visit this topic in live forum Printer Friendly Version Pages: Previous 1 2 epi123 on Mar 26 2010, 05:51 AM said:happy to see you got your profile. keep in mind if your are planning on sequencing, that chelex resin ...
http://www.protocol-online.org/biology-forums-2/posts/13784more1.html 05/24/13, 12922 bytes

ChIP sonication problem: too wide range of smear - ChIP: CHIP SONICATION PROBLEM: TOO WIDE RANGE OF SMEAR - TOO WIDE RANGE OF SMEAR (JUN/05/2006 ) Hi All, I am just starting to do ChIP. My problem with the sonication is that when I tested the sonicated DNA on 1% gel, I saw a smear ranging from 200 bp to 4kb (even after I treated with RNase A). There were ...
http://www.protocol-online.org/biology-forums/posts/17017.html 02/17/13, 12739 bytes

ChIP: Sonication - DNA Methylation, Histone and Chromatin Study: CHIP: SONICATION - (DEC/16/2005 ) Hello, I have been using EZ ChIPTM protocol from Upstate. I have been trying to find the optimal conditions for sonication, but I have problems to do it. I use an ultrasonic homogenizer, model 150 V/T (Biologics) at about 40% of maximum power (I suppose it, because ...
http://www.protocol-online.org/biology-forums/posts/12124.html 02/17/13, 8725 bytes

Sonication issues! - Protein Expression and Purification: SONICATION ISSUES! - (MAR/01/2010 ) Visit this topic in live forum Printer Friendly Version Following sonciation of my cells, I centrifuged as normal to harvest the insoluble protein fraction. Only to find I couldn't get a significant pellet no matter how much I spun the samples down&#33 ...
http://www.protocol-online.org/biology-forums-2/posts/13404.html 05/24/13, 11283 bytes

Sonication mysteries - Protein and Proteomics: SONICATION MYSTERIES - (MAR/08/2010 ) Visit this topic in live forum Printer Friendly Version Hello! I'm in the "getting organized" phase of a secretome analysis on some mouse middle ear epithelials. The plan is to treat them with NTHi lysates, which I'm in the process of making. The ...
http://www.protocol-online.org/biology-forums-2/posts/13554.html 05/24/13, 13657 bytes

ChIP DNA sonication conditions - DNA Methylation, Histone and Chromatin Study: CHIP DNA SONICATION CONDITIONS - (OCT/18/2005 ) Hi all! I need to sonicate a DNA sample from a chromatin immunoprecipitation into 200 bp fragments, but I do not really know which must be the sonication conditions. I do not get fragments less than 2000 bp. I am using a Vibra Cell from SONICS. I ...
http://www.protocol-online.org/biology-forums/posts/10430.html 02/17/13, 9491 bytes

sonication of PBMC - Immunology: sonication of PBMC - (Feb/05/2001 ) sir, I am in desperate need of a protocol for the sonication of PBMC for animportant experiment, where I would ultimately do a Western Blot with the protein extract . Please forward any advise with this regard to my e-mail address. -- PBMC isolation has to be carried ...
http://www.protocol-online.org/biology-forums-2/posts/1346.html 08/21/11, 10201 bytes

Condition for sonication of protein solution to get rid of DNA? - Protein and Proteomics: CONDITION FOR SONICATION OF PROTEIN SOLUTION TO GET RID OF DNA? - HOW LONG TO SONICATE AND WITH WHAT AMPLITUDE? (DEC/14/2005 ) I have 80 ml of my periplasmic fluid with overexpressed protein in it. To put in on th Ni column I need to get rid of DNA which makes my colution viscous. I think the sonication ...
http://www.protocol-online.org/biology-forums/posts/12058.html 02/17/13, 10334 bytes

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