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Search results for '(northern or northerner or northernly or northerners) and (blot or blots)'
Documents 1 - 10 of 70 matches.
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- Northern blotting versus southern blotting - General Lab Techniques
- Northern blotting versus southern blotting - use of formamide (Nov/14/2009 ) Dear all, I have the following question: I received a protocol both blottings methodes. And for the northern blotting one step is to use formamide to prevent or reduce intermolecular basepairing. Now I am wondering: why do
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http://www.protocol-online.org/biology-forums-2/posts/11540.html 12/10/12, 15581 bytes
- qrt-pcr and northern blot ,which way is better? - siRNA, microRNA and RNAi
- qrt-pcr and northern blot ,which way is better? - (Jan/14/2010 ) Hi, I'm new to microRNA.I want to confirm the deep sequencing results.qrt-pcr and northern blot ,which way is better? Thanks -luc- luc on Jan 14 2010, 02:09 PM said:Hi, I'm new to microRNA.I want to confirm the deep sequencing
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http://www.protocol-online.org/biology-forums-2/posts/12582.html 12/10/12, 11204 bytes
- Northern blot with mRNA - Molecular Biology
- Northern blot with mRNA - (Feb/17/2010 ) Hi, there! I would like to detect some transcripts spliced forms of my gene, so I was thinking in detecting them by northern blot, but using mRNA instead of total RNA. I was considering two possibilities: 1.- Extraction of total RNA from my cells, and then
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http://www.protocol-online.org/biology-forums-2/posts/13190.html 12/10/12, 11307 bytes
- Positive control for Northern Blot - Molecular Biology
- Positive control for Northern Blot - (Jul/14/2010 ) Hi everyone, my boss ask me to have a good positive control for my northern blot. I did a lot of lecture but I just saw negatif control. So do you have a idea of what's a good controlo for a northern? thanks -Xavier- Good positive control is something
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http://www.protocol-online.org/biology-forums-2/posts/15951.html 12/10/12, 10067 bytes
- labeling on southern, northern and western blot - Molecular Biology
- labeling on southern, northern and western blot - (May/02/2001 ) What is the commonly use label (except radioactive thing) for probe for Southern, Northern and Western blot today? And how the label expose to a hard copy? -- For the most part, Southerns and Northerns still rely on radioactivity. It
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http://www.protocol-online.org/biology-forums-2/posts/1118.html 08/21/11, 10387 bytes
- Northern blot - Molecular Biology
- Northern blot - (Feb/11/2010 ) Hello, I'm doing northern using AMBION kit using the biotin assay I have few problems: 1. How can I save my membrane wet if in the procedure there is RNA crosslink in 80 degree oven? 2. Tomorrow I need to develop with the BrightStar kit so first do I need to put the
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http://www.protocol-online.org/biology-forums-2/posts/13117.html 12/10/12, 10873 bytes
- microrna quantitation ---use northern blot or qrt-pcr？ - Molecular Biology
- microrna quantitation ---use northern blot or qrt-pcr？ - (Jan/13/2010 ) I‘m just starting to work with miRNA and would be very grateful if you could give me some advice. -luc- Hi Now sure have you heard of Agilent small RNA assay using Bioanalyzer? It is quick an sensitive method/ assays which
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http://www.protocol-online.org/biology-forums-2/posts/12577.html 12/10/12, 10041 bytes
- DNA ladders on a northern blot - General Lab Techniques
- DNA ladders on a northern blot - (Sep/19/2009 ) Hi all, I was wondering if someone could tell me if this would work; Run my total RNA sample (looking for miRNA) with a DNA ladder on a denaturing gel. The reason I want to use a DNA ladder is that I can label it with dye and image it and my labeled probe
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http://www.protocol-online.org/biology-forums-2/posts/10382.html 12/10/12, 10469 bytes
- miRNA northern - Molecular Biology
- miRNA northern - (Jan/25/2010 ) ' href="http://www.protocol-online.org/forums/index.php?app=core&module=attach§ion=attach&attach_rel_module=post&attach_id=1217" target="_blank"> Attached Image im trying to run a northern on miRNA and since U6 and 5S rRNA seems to be the most widely used endogenous
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http://www.protocol-online.org/biology-forums-2/posts/12813.html 12/10/12, 11681 bytes
- northern prob - Molecular Biology
- northern prob - (Jan/28/2010 ) Hi, I'm trying to figure out how to design a northern blot, I have DNA in Topo TA cloning vector with T7 promoter so what is the next step should I use Prime a Gene to label it and I don't really understand the non label - how do you detect the hybridization?
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http://www.protocol-online.org/biology-forums-2/posts/12877.html 12/10/12, 11738 bytes
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