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Documents 1 - 10 of 12 matches.
'Black dots' in cell culture - Tissue and Cell Culture****
'Black dots' in cell culture - (Feb/02/2010 ) Hi everyone, We have an issue in our tissue culture room. There are two of us with suspension cell lines, and two with adherent. None of us use antibiotics at the moment in our cultures. Just before Christmas, I noticed tiny 'fidgeting' ...
http://www.protocol-online.org/biology-forums-2/posts/12942.html 12/10/12, 11929 bytes
Northern blot background dots - Molecular Biology***
Northern blot background dots - (Dec/17/2005 ) Hello I have been consistently getting lots of small dark dots all over my northern blots. My bands and marker are present, but the little dots make the blot quite ugly. Has anyone seen this before and know how to get rid of it? Here is some info about ...
http://www.protocol-online.org/biology-forums/posts/12139.html 02/01/09, 10551 bytes
Moving Dots in cell culture - Contamination? - Cell Biology***
Moving Dots in cell culture - Contamination? - (Jan/09/2006 ) I have noticed black dots in my cell culture. They fidget, but do not move from one place to another. When I left cultures overnight, the medium (DMEM) was not clouded (as it usually is upon bacterial contamination) and the contamination ...
http://www.protocol-online.org/biology-forums/posts/12608.html 02/01/09, 12575 bytes
Contamination in SH-SY5Y cultures, mycoplasma? - Tissue and Cell Culture**
... other bacteria-) infection of SH-SY5Y cells? How did the cells look and react to that? I have a contamination in my cultures which shows as a lot of black dots in the cells cytoplasm. In some dishes, also the media contains moving black dots. It also seems as the cells are growing at a slower rate than ...
http://www.protocol-online.org/biology-forums-2/posts/15197.html 12/10/12, 11105 bytes
Contamination Paranoia - Cell Biology**
... like bacteria to me now (Oct/20/2005 ) It was a quiet contamination problem. Apparently, the bacteria had been there all along, exisiting as little black dots in the cultures that nobody would have noticed unless looking for them. Only after a flask had been neglected for a week would we start to see ...
http://www.protocol-online.org/biology-forums/posts/10538.html 02/01/09, 13364 bytes
Contamination doesn't grow - Tissue and Cell Culture**
... (the pic in my post). This time the cells seem to be dying off and have not attached either ;) The puzzling thing is neither the cells nor the 'black dots' seem to be growing. BTW I'm working with RIN 5F cells (rat beta cells). -Kitty- Kitty on Apr 14 2010, 07:56 PM said:Thank you for ...
http://www.protocol-online.org/biology-forums-2/posts/14250.html 12/10/12, 24309 bytes
No signal at all - SDS-PAGE and Western Blotting**
No signal at all - (Jan/24/2010 ) Pages: 1 2 Next Hi everyone, Recently, my western and dot blotting never gave me any signal. The film looked very clear without any marking of membrane left. Then, we tried to do the dot blot with the same concentration of primary and secondary antibody (1: 20.000 ...
http://www.protocol-online.org/biology-forums-2/posts/12781.html 12/10/12, 21087 bytes
Keeping check of an In-situ hybridisation experiment in general and making good - Molecular Biology**
... (i.e. purification) is golden. Now In my graduate research Im facing whole-mount In-situ hybridisation. Itīs a long protocol and it seems rather "black-boxy" to me. See, you only get to kno wheter the experment worked or not after a weeks worth of work. And then you have all the parameters to play with ...
http://www.protocol-online.org/biology-forums-2/posts/10337.html 12/10/12, 12931 bytes
Recovery of HeLa cells - Cell Biology*
... itself, i realised that i shd have added cells dropwise to the medium, however my cells just die within 24 hrs. my other question is, if we see black (very) tiny spots or black spikes on top of the adhered cells ((floating))....in the dish ...what does this signify ??? is it bacterial contamination ...
http://www.protocol-online.org/biology-forums/posts/13100.html 02/01/09, 14025 bytes
Urgent ! Help for infection ... - Cell Biology*
Urgent ! Help for infection ... - Many dark spots appear beside my cell (Mar/24/2006 ) A month ago, I have found some black spots appeared in my culture disk which besides the cells. Somestimes they are floating on the top of the medium result in forming a large area pictures like (infection figure ...
http://www.protocol-online.org/biology-forums/posts/14941.html 02/01/09, 10241 bytes

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