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Search results for '(antibody or antibodies)'
Documents 1 - 10 of 890 matches.
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- Mock IgG versus No Antibody - Negative Control - ChIP and Next Generation Sequencing
- Mock IgG versus No Antibody - Negative Control - (Dec/10/2009 ) I was just wondering what different people's thoughts were on using a mock antibody (e.g, Rabbit IgG) versus no antibody for their negative control in ChIP. I'm currently working with a mock anitbody (Rabbit IgG from Cell Signaling
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http://www.protocol-online.org/biology-forums-2/posts/12058.html 12/10/12, 18310 bytes
- sample gives signal without capture antibody - ELISA and Immunoassay
- sample gives signal without capture antibody - No capture antibody but samples give high signal compared to blank (Feb/19/2010 ) Hello, I am optimizing a sandwich ELISA to detect the amount of antigen in CSF samples. I coated wells with capture antibody and added CSF samples and added detection antibody
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http://www.protocol-online.org/biology-forums-2/posts/13251.html 12/10/12, 13882 bytes
- non-specific bands - SDS-PAGE and Western Blotting
.../01/2010 ) Hi all, I am just wondering if anyone knows of how to tell if you have non specific bands on your blot due to lack of specificity of the antibodies. I originally had antibody from santa cruz but then was told that the antibody for the particular transcription factor I'm looking at might...
http://www.protocol-online.org/biology-forums-2/posts/14608.html 12/10/12, 16674 bytes
- Good antibody for E. coli surface marker - Flow Cytometry
- Good antibody for E. coli surface marker - phagocytosis assay (Feb/11/2010 ) Hey guys, I am doing phagocytosis assay with macrophages and my GFP-E. coli. In order to distinguish between intracellular and extracellular bacteria after infection, I need to label the extracellular bacteria with an antibody
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http://www.protocol-online.org/biology-forums-2/posts/13120.html 12/10/12, 13030 bytes
- primary antibody for western - General Lab Techniques
- primary antibody for western - (Mar/01/2010 ) hi, Is it possible that an antibody works for immunofluorescence and not for western blot???? in fact, my immunofluorescence was good even the intensity of the protein was not strong! but by western blot, i could'nt see my protein even by repeting
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http://www.protocol-online.org/biology-forums-2/posts/13413.html 12/10/12, 14041 bytes
- Troubleshooting Help - Sandwich ELISA - ELISA and Immunoassay
...;ll find below is a decently well planned system. I ran an initial run of the system that I am trying to design and got extremely high OD in capture antibody-negative wells and protein blank wells. I’ll describe the system below. If anyone could posit a potential problem I’d be so grateful! Capture...
http://www.protocol-online.org/biology-forums-2/posts/11709.html 12/10/12, 29080 bytes
- weak purified antibody - Immunology
- weak purified antibody - (Apr/07/2010 ) Dear all, I purified my protein by using agarose A, the SDS PAGE showed the bands related to my antibody ( light and heavy) and when I used t in immunostaining the staining was very faint !! can anyone give me explenation. -bluebird- Have you measured
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http://www.protocol-online.org/biology-forums-2/posts/14109.html 12/10/12, 11901 bytes
- Antibody staining - Flow Cytometry
- Antibody staining - (Nov/11/2009 ) Hi, I'm going to do several FACS experiments using between 2 and 4 antibodies. My question may appear maive but I'm wondering if I should add each antibody to each sample separately or if I can make an antibody mix and then add the mix to each sample. Is there
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http://www.protocol-online.org/biology-forums-2/posts/11469.html 12/10/12, 14745 bytes
- Generation of ChIP grade antibody - ChIP and Next Generation Sequencing
- Generation of ChIP grade antibody - Purification of recombinant protein (May/12/2010 ) Hi everyone! I am working on a transciption factor but the available commercial antibodies do not work well in ChIPs, so we have decided to generate our own polyclonal antibody. I have cloned the desirable region
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http://www.protocol-online.org/biology-forums-2/posts/14803.html 12/10/12, 13828 bytes
- Is my antibody ChIP grade? - ChIP and Next Generation Sequencing
- Is my antibody ChIP grade? - (Mar/10/2010 ) Pages: 1 2 Next What's the general consensus as to what constitutes a ChIP grade antibody? What experiments do I have to perform to prove that my antibody is ChIP grade? Thanks! -nigelcr- nigelcr on Mar 10 2010, 08:26 AM said:What's the general
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http://www.protocol-online.org/biology-forums-2/posts/13618.html 12/10/12, 25954 bytes
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